Author(s):
Dipendra Nirmalkar, Aarti Tiwari, Gulshan Athbhaiya, Pradeep Kumar Samal
Email(s):
samalpharmacology@rediffmail.com
DOI:
10.52711/0974-360X.2025.00093 
Address:
Dipendra Nirmalkar, Aarti Tiwari, Gulshan Athbhaiya, Pradeep Kumar Samal*
Department of Pharmacy, Guru Ghasidas Vishwavidyalaya, Bilaspur, Chhattisgarh, 495009, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Aim: - The purpose of this study was to evaluate the hepatoprotective properties of Lathyrus sativus leaves against liver damage caused by paracetamol in Wistar rats. Methods: -Rats with hepatotoxicity from paracetamol were utilised to test the hepatoprotective benefits of a methanol extract made from Lathyrus sativus leaves. To cause hepatotoxicity, albino Wistar rats were fed paracetamol (2 g/kg) orally for 14 days. For 14 days, oral administration of the Lathyrus sativus leaf methanol extract at 200 and 400 mg/kg/day was conducted. The quantities of albumin, total bilirubin, ALT, AST, ALP, and protein were found using a serum analysis. To measure the amounts of glutathione, malondialdehyde, and superoxide dismutase, the liver was removed and ground up. Furthermore, investigations related to histology were performed on the liver samples. Results: The groups who received extract treatment were able to reduce the negative effects of paracetamol while restoring blood biochemical parameters to levels that were nearly normal. Conclusions: - According to the study, Lathyrus sativus leaves have significant hepatoprotective effects.
Cite this article:
Dipendra Nirmalkar, Aarti Tiwari, Gulshan Athbhaiya, Pradeep Kumar Samal. Hepatoprotective Activity of Lathyrus sativus Leaf Extract in Paracetamol-Induced Hepatotoxic Rats. Research Journal of Pharmacy and Technology.2025;18(2):625-1. doi: 10.52711/0974-360X.2025.00093
Cite(Electronic):
Dipendra Nirmalkar, Aarti Tiwari, Gulshan Athbhaiya, Pradeep Kumar Samal. Hepatoprotective Activity of Lathyrus sativus Leaf Extract in Paracetamol-Induced Hepatotoxic Rats. Research Journal of Pharmacy and Technology.2025;18(2):625-1. doi: 10.52711/0974-360X.2025.00093 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-25
8. REFERENCES:
1. J. Ozougwu. Physiology of the liver. Int. J. Res. Pharm. Biosci. 2017; 4(8): 13-24.
2. K. G. Wakim. Physiology of the liver. Am. J. Med. 1954; 16(2): 256–271, doi: 10.1016/0002-9343(54)90342-3.
3. D. Mondal, K. Das, and A. Chowdhury. Epidemiology of Liver Diseases in India. Clin. Liver Dis. 2022; 19(3): 114–117 doi: 10.1002/cld.1177.
4. H. Y. Tong et al. Hepatotoxicity induced by acute and chronic paracetamol overdose in children: Where do we stand? World J. Pediatr. 2017; 13(1): 76–83 doi: 10.1007/s12519-016-0046-6.
5. M. Umadevi, C. Maheswari, R. Jothi, S. K. Paleti, Y. Srinivasa Reddy, and R. Venkata Narayanan. Hepatoprotective activity of flowers of Madhuca longifolia (Koen.) Macbr. against paracetamol-induced hepatotoxicity. Res. J. Pharm. Technol. 2011; 4(2); 259–262.
6. J. Fernández et al. Antiproliferative and palliative activity of flavonoids in colorectal cancer. Biomed. Pharmacother. 2021; 143. doi: 10.1016/j.biopha.2021.112241.
7. H. Farghali, N. K. Canová, and S. Zakhari. Hepatoprotective properties of extensively studied medicinal plant active constituents: Possible common mechanisms. Pharm. Biol. 2015; 53(6): 781–791 doi: 10.3109/13880209.2014.950387.
8. P. K. Samal, J. S. Dangi, and K. P. Meena. Ethnopharmacological studies of traditional hepatoprotective medicinal plants used by the tribals of Bilaspur districts. Int. J. Pharm. Technol. 1917; 3(2): 1917–1987.
9. N. R. Beck and P. Samal. Traditional medicinal plants used by the tribes and rural people of Bilaspur District, Chhattisgarh (India). Res. J. Pharm. Technol. 2012; 5(10): 1281–1282.
10. A. E. Al-snafi. Chemical constituents and pharmacological effects of Ocimumbasilicum- A review. Int. J. Pharm. Res. 2021; 13(2): 51–58 doi: 10.31838/ijpr/2021.13.02.388.
11. R. Jeyadevi, D. A. Ananth, and T. Sivasudha. Hepatoprotective and antioxidant activity of Ipomoea staphylina Linn. Clin. Phytoscience. 2019; 5(1). doi: 10.1186/s40816-019-0112-4.
12. H. Ohkawa, N. Ohishi, and K. Yagi. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 1979; 95(2): 351–358 doi: 10.1016/0003-2697(79)90738-3.
13. L. C. Green, D. A. Wagner, J. Glogowski, P. L. Skipper, J. S. Wishnok, and S. R. Tannenbaum. Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal. Biochem. 1982; 126(1): 131–138 doi: 10.1016/0003-2697(82)90118-X.
14. P. Kakkar, B. Das, and P. N. Viswanathan. A modified spectrophotometric assay of superoxide dismutase. Indian J. Biochem. Biophys. 1984; 21(2); 130–132.
15. G. L. Ellman. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 1959; 82(1): 70–77 doi: 10.1016/0003-9861(59)90090-6.
16. R. D. Goldin, C. S. Breach, I. N. Brown, and S. N. Wickramasinghe. Role of Macrophages in Acetaminophen. 1996; 179: 432–435.
17. J. A. Hinson, T. J. Bucci, L. K. Irwin, S. L. Michael, and P. R. Mayeux. Effect of inhibitors of nitric oxide synthase on acetaminophen-induced hepatotoxicity in mice. Nitric Oxide - Biol. Chem. 2002; 6(2): 160–167 doi: 10.1006/niox.2001.0404.
18. P. K. Samal. Hepatoprotective Activity of Tamarindus indica on Liver Damage Caused by Thioacetamide. Res. J. Pharmacogn. Phytochem. 2013; 5(3): 123–126
19. R. Tittarelli et al. Hepatotoxicity of paracetamol and related fatalities. Eur. Rev. Med. Pharmacol. Sci. 2017; 21(1): 95–101.
20. P. K. Samal. Hepatoprotective activity of Ardisia solanacea in CCl 4 induced Hepatoxic albino rats. RJPT. 2013; 3(2): 79–82.
21. Z. A. Zakaria, N. D. Mahmood, M. H. Omar, M. Taher, and R. Basir. Methanol extract of Muntingia calabura leaves attenuates CCl4-induced liver injury: possible synergistic action of flavonoids and volatile bioactive compounds on endogenous defence system. Pharm. Biol. 2019; 57(1): 335–344 doi: 10.1080/13880209.2019.1606836.
22. P. K. Samal. Antioxidant activity of Strobilanthes asperrimus in albino rats. Pharm. Res. 2013; 3: 71–74 [Online]. Available: www.asianpharmaonline.org
23. P. K. Samal. Investigation of Antioxidant activity of Butea monosperm barks. Res. J. Pharm. Technol. 2013; 6(6): 610–613.
24. J. Ozer, M. Ratner, M. Shaw, W. Bailey, and S. Schomaker. The current state of serum biomarkers of hepatotoxicity. Toxicology. 2008; 245(3): 194–205 doi: 10.1016/j.tox.2007.11.021.
25. P. K. Samal. Assessment of hepatoprotective activity of Strobilanthes asperrimus in thioacetamide induced hepatoxic rats. Res. J. Pharmacogn. Phytochem. 2013; 5(3): 139–142.
26. K. A. Amin, B. M. Mohamed, M. A. M. El-Wakil, and S. O. Ibrahim. Impact of breast cancer and combination chemotherapy on oxidative stress, hepatic and cardiac markers. J. Breast Cancer. 2012; 15(3): 306–312 doi: 10.4048/jbc.2012.15.3.306.
27. D. Verma and P. K. Samal. Evaluation of synergistic analgesic activity of berberine and asiatic acid in mice. Res. J. Pharm. Technol. 2020; 13(12): 6081–6085 doi: 10.5958/0974-360X.2020.01060.4.
28. G. Dayanand Reddy et al. Hepatoprotective activity of Aruvadha churnam a traditional siddha formulation against paracetamol induced liver damage in rats. Res. J. Pharm. Technol. 2018; 11(8): 3380–3384 doi: 10.5958/0974-360X.2018.00622.4.
29. F. A. Armah et al. Allanblackia floribunda Seed Extract Attenuates the Ethanol-Induced Gastric Ulcer in Rats via the Inhibition of TNF- α and INF- γ Levels and Modulation in the Expression of Ki67 Protein. Biomed Res. Int. 2021 doi: 10.1155/2021/6694572.
30. M. T. Islam et al. Effects of nerol on paracetamol-induced liver damage in Wistar albino rats. Biomed. Pharmacother. 2021; 140: 111732 doi: 10.1016/j.biopha.2021.111732.
31. M. T. Yakubu, L. S. Bilbis, M. Lawal, and M. A. Akanji. Evaluation of selected parameters of rat liver and kidney function following repeated administration of yohimbine. Niger. Soc. Exp. Biol. 2003; 15: 50–56.
32. J. L. Anoopa, N. Kannappan, and P. Manojkumar. Evaluation of hepatoprotective activity in methanolic extract of aerial parts of Hibiscus surattensis. Res. J. Pharm. Technol. 2020; 13(10): 4635 doi: 10.5958/0974-360x.2020.00816.1.
33. J. Patel, A. Venkateshwar Reddy, G. S. Kumar, D. Satyasai, B. Bajari, and V. Nagarjuna. Hepatoprotective activity of methanolic extract of Terminalia coriacea leaves. Res. J. Pharm. Technol. 2017; 10(5): 1313–1316 doi: 10.5958/0974-360X.2017.00232.3.
34. M. S. Taju G, Jayanthi M. Evaluation of hepatoprotective and antioxidant activity of Psidium guajava leaf extract against acetaminophen-induced liver injury in rats. Int. J. Toxicol. Appl. Pharmacol. 2011: 13–20.
35. P. K. Samal, J. S. Dangi, K. P. Meena, N. R. Beck, G. Maheshwari, and A. Patel. Hepatoprotective activity of Butea monosperma bark on liver damage caused by paracetamol in rats. Res. J. Pharm. Technol. 2011; 4(5): 771–774.
36. T. S. Rangnathrao and P. Shanmugasundaram. Antioxidant and hepatoprotective activity of Ehretia laevis Roxb against paracetamol induced acute hepatotoxicityin wistar rats. Res. J. Pharm. Technol. 2019; 12(12); 6143–6148 doi: 10.5958/0974-360X.2019.01067.9.
37. S. L. Michael, N. R. Pumford, P. R. Mayeux, M. R. Niesman, and J. A. Hinson. Pretreatment of mice with macrophage inactivators decreases acetaminophen hepatotoxicity and the formation of reactive oxygen and nitrogen species. Hepatology. 1999; 30(1): 186–195 doi: 10.1002/hep.510300104.
38. J. A. Hinson, A. B. Reid, S. S. McCullough, and L. P. James. Acetaminophen-induced hepatotoxicity: Role of metabolic activation, reactive oxygen/nitrogen species, and mitochondrial permeability transition. Drug Metab. Rev. 2004; 36(3–4): 805–822 doi: 10.1081/DMR-200033494.
39. M. S. Rahman et al. Ethnobotanical uses, phytochemistry and pharmacological activities of pterocarpus marsupium: A review. Pharmacogn. J. 2018; 10(6): S1–S8 doi: 10.5530/pj.2018.6s.1.
40. P. K. Samal, J. S. Dangi, K. P. Meena, N. R. Beck, A. Patel, and G. Maheshwari. Evaluation of analgesic activity of leaves extracts of Feronia limonia in experimental animal models. Res. J. Pharm. Technol. 2011; 4(5): 710–714.
41. M. R. Manokaran S, Jaswanth A, Sengottuvelu S, Nandhakumar J, Duraisamy R, Karthikeyan D. Hepatoprotective activity of Aerva lanata Linn. against paracetamol induced hepatotoxicity in rats. Res. J. Pharm. Technol. 2008; 1(4): 398–401.