Author(s):
Mohini Salunke, Preeti Mane, Smita Kumbhar, Balaji Wakure
Email(s):
mohinisaluke82@gmail.com
DOI:
10.52711/0974-360X.2025.00086 
Address:
Mohini Salunke1*, Preeti Mane2, Smita Kumbhar3, Balaji Wakure1
1Vilasrao Deshmukh Foundation, Group of Institutions, VDF School of Pharmacy, Latur - 413 531, Maharashtra, India.
2Department of Pharmacy, Terna Public Charitable Trust’s, College of Engineering, Osmanabad, India.
3Sanjivani College of Pharmaceutical Education and Research, Kopargaon, Maharashtra, India.
*Corresponding Author
Published In:
Volume - 18,
Issue - 2,
Year - 2025
ABSTRACT:
Non-specific phytochemical profiling of plant extracts will help in the study of different groups of compounds and discovery of new bioactives, thereby minimising compound identification errors. The pharmaceutical industry has the potential to develop innovative drugs by using the large range of bioactive metabolites that marine seaweed is known to produce. Analysis of complex bioactive ingredients using analytical methods such as HR-LCMS has been demonstrated to be significant. In this research, we used HR-LCMS and FTIR analysis to explore at the metabolic profiles of Turbinaria ornata. The current investigation verifies the existence of significant therapeutic bioactive substances, such as Terpene Glycosides, Beta-Keto Acid, Steroid, Lipids, Glycosides, Triterpenoid, Phenols, Flavonoid, Quinolizidine Alkaloid, Vitamin, and Oligopeptides and shown to have antimicrobial, antioxidant, anti-inflammatory, antitumorproperties. The results of this investigation demonstrate that Turbinaria ornata contains significant phytocompounds and useful for more in-depth research to create marine algae-derived medicines to treat a variety of ailments.
Cite this article:
Mohini Salunke, Preeti Mane, Smita Kumbhar, Balaji Wakure. HR-LCMS and FTIR characterization of bioactive components of Turbinaria ornata. Research Journal of Pharmacy and Technology.2025;18(2):579-4. doi: 10.52711/0974-360X.2025.00086
Cite(Electronic):
Mohini Salunke, Preeti Mane, Smita Kumbhar, Balaji Wakure. HR-LCMS and FTIR characterization of bioactive components of Turbinaria ornata. Research Journal of Pharmacy and Technology.2025;18(2):579-4. doi: 10.52711/0974-360X.2025.00086 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-2-18
REFERENCES:
1. Kumar P, Senthamilselvi S, Govindaraju M. GC–MS profiling and antibacterial activity of Sargassum tenerrimum. J Pharm Res. 2013; 6(1): 88–92.
2. Rajauria G, Abu-Ghannam N. Isolation, and partial characterization of bioactive fucoxanthin from himanthaliaelongata brown seaweed: A TLC-based approach. Int J Anal Chem. 2013;
3. Ragunathan V, Pandurangan J, Ramakrishnan T. Gas Chromatography-mass spectrometry Analysis of Methanol Extracts from Marine Red Seaweed Gracilaria corticata. Pharmacognosy Journal. 2019; 11(3): 547–554.
4. Ahilya V. Waghmode PMS. Gas chromatography-high resolution mass spectrometry analysis of Sargassum species. Indian Hydrobiolog. [homepage on the Internet] 2021; 20(1): 35–44. Available from: www.krishalgology.yolasite.com
5. Remya RR, Radhika Rajasree SR. A study on bioactive compounds derived from brown seaweeds and their therapeutic applications towards various diseases. Res J Pharm Technol. 2016; 9(4): 369–372.
6. Thiruchelvi. R KK, Shankari K. New Biotechnological Routes for Greener Bio-plastics from Seaweeds. Research J Pharm and Tech. [homepage on the Internet] 2020 [cited 2024 Jan 22]; 13(5): 2488–2492. Available from: 10.5958/0974-360X.2020.00444.8
7. Zhong B, Robinson NA, Warner RD, Barrow CJ, Dunshea FR, Suleria HAR. LC-ESI-QTOF-MS/MS characterization of seaweed phenolics and their antioxidant potential. Mar Drugs. 2020; 18(6).
8. Chen S, Sathuvan M, Zhang X, et al. Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis. BMC Chem. 2021; 15(1).
9. Umavandhana R, Jayanthi S. Phytochemical screening and free radical scavenging activity on some selected seaweeds from Gulf of Mannar, India. Res J Pharm Technol. 2018; 11(8): 3385–3388.
10. Deepak P, Sowmiya R, Balasubramani G, Perumal P. Phytochemical profiling of Turbinaria ornata and its antioxidant and anti-proliferative effects. J Taibah Univ Med Sci. 2017; 12(4): 329–337.
11. Olate-Gallegos C, Barriga A, Vergara C, et al. Identification of Polyphenols from Chilean Brown Seaweeds Extracts by LC-DAD-ESI-MS/MS. Journal of Aquatic Food Product Technology. 2019;28(4):375–391.
12. Marulasiddaswamy KM, Nuthan BR, Sunilkumar CR, et al. Hr-lc-ms based profiling of phytochemicals from methanol extracts of leaves and bark of myristicadactyloidesgaertn. From western ghats of karnataka, india. J Appl BiolBiotechnol. 2021; 9(5):124–135.
13. Agardh C, Agardh J, Cyriac B, Eswaran K. GC-MS determination of bioactive components of Gracilaria dura. Science Research Reporter. [homepage on the Internet] 2015; 5(2): 100–105. Available from: http://jsrr.net
14. Salunke M, Wakure B, Wakte P. Hyphenated Techniques for The Characterization of Seaweed Bioactive Compounds. Res J Pharm Technol. 2023; 16(9): 4455–4461.
15. Thiruchelvi R, Jayashree P, Hemashree T, Hemasudha TS, Balashanmugam P. Preliminary phytochemical analysis of the crude extract of marine red and brown seaweeds. Res J Pharm Technol. 2018; 11(10): 4407–4410.
16. Shmygareva AA, Kurkin VA, Sankov AN, Rybalko MV, Semeniuta KN. Method of obtaining of extract by the method of modified maceration. Res J Pharm Technol. 2019; 12(12): 5956–5958.
17. Salunke M, Wakure B, Wakte P. HR-LCMS assisted phytochemical screening and an assessment of anticancer activity of Sargassum Squarrossum and Dictyota Dichotoma using in vitro and molecular docking approaches. J Mol Struct. 2022; 1270.
18. Chethankumara GP, Krishna V, Nagaraj K. HR-LCMS and In vitro cytotoxicity analysis of Alseodaphnesemecarpifolia stem bark and leaf methanol extracts. Research J Pharm and Tech. [homepage on the Internet] 2022 [cited 2024 Jan 22]; 15(1): 250–256. Available from: 10.52711/0974-360X.2022.00041
19. Deliza H, Ningombam D, Maibam D. Elemental and Phytochemical composition of Pratiabegonifolia (Wall.) Lindl. by using GF-AAS, SEM-EDAX, FTIR, GC-MS and HR-LCMS. Research J Pharm and Tech. 2022 [cited 2024 Jan 22]; 16(4): 1556–1560. Available from: 10.52711/0974-360X.2023.00254
20. Neupane P, Lamichhane J. Phytochemical profiling using HRLCMS and evaluation of antioxidant and antibacterial activities of Nepalese medicinal plants. Vegetos. 2020; 33(4): 628–640.
21. Salunke MA, Wakure BS, Wakte PS. High-resolution liquid chromatography and mass spectrometry (HR-LCMS) assisted phytochemical profiling and an assessment of anticancer activities of Gracilaria foliifera and Turbinaria conoides using in vitro and molecular docking analysis. J Biomol Struct Dyn. 2022;1–16.
22. Naganathan S, Natarajan A, Vivek P, Kesavan D, Ivo Romauld S. In silico anti-HIV analysis of FTIR identified bioactive compounds present in Vitex altissima L and Vitex leucoxylon L. Res J Pharm Technol. 2019; 12(4): 1773–1782.
23. Salunke M, Wakure B, Wakte P. Phytochemical Screening of Marine Brown Algae Sargassum squarrossum Greville. 2022;
24. Salunke MA, Wakure BS, Wakte PS. Phytochemical, UV-VIS, and FTIR Analysis of Gracilaria foliifera. Res J Pharm Technol. 2023; 16(3): 1391–1394.
25. Salunke Mohini, Wakure Balaji, Wakte Pravin. Phytochemical analysis of Acanthophora najadiformis using High-Resolution Liquid Chromatography Mass Spectrometry (HR-LCMS) and FTIR. Journal of Pharmaceutical Negative Results. 2022; 13(6): 2215–2218.
26. Salunke M, Wakure B, Wakte P. High-resolution liquid chromatography mass spectrometry (HR-LCMS) and 1H NMR analysis of methanol extracts from marine seaweed Gracilaria edulis. Nat Prod Res. [homepage on the Internet] 2022; 1–4. Available from: https://www.tandfonline.com/doi/ full/10.1080/14786419.2022.2146906