ABSTRACT:
Antidesma thwaitesianum Müll. Arg. or "Mamao," is tropical plants that prominently cultivated in South-East Asia. Recently, the fruit juice is become the favourited local products in the North-Eastern of Thailand as nutraceutical product for healthy consumers. The pomace from juice processing industries is the valuable and attractive source of antioxidant and bioactive compounds for ingredient skin care products. Therefore, the biological activities were sparely reported. This research was aimed to screening of bioactive constituents of Mamao pomace ethanol extract (MPE), such as phenolic, flavonoid and tannin contents and to evaluate its in vitro biological activities for skin care and treatment. The MPE was viscous liquid with purplish red coloured, and the yield of extraction was about 5%. Total phenolic and total tannin contents of MPE were 23.77±0.37mg GAE/g and 166.73±2.7mg TE/g, respectively, therefore, total flavonoid content was undetectable. The MPE was exhibited DPPH scavenging activity (IC50 = 0.12±0.02mg/ml) and poorly chelated metal ion and inhibited lipid peroxidation. While it was unable to scavenge NO radical and inhibiting tyrosinase and elastase. The MPE (5 mg) was slightly inhibited Cutibacterium acnes (inhibition zones = 10.06±0.04mm). The MPE (1.0mg/ml) was lack of toxicity against human skin fibroblasts. The MPE(0.1mg/ml) was inhibited 18.94±1.96% of NO production from LPS-induced macrophage. For wound healing property, MPE(1.0mg/ml) was reduced 84.97±3.34% of the gap length of skin fibroblast cluster after 48 h treatment.