Author(s):
David Tjahyadi, Edy Parwanto, Hosea Jaya Edy, Joey Joshua Vidova Tjahyadi, Laurentia Gabrielle, Ashaolu Victoria Oladimeji, Seçil Karahüseyin
Email(s):
davesaboch@trisakti.ac.id
DOI:
10.52711/0974-360X.2025.00704 
Address:
David Tjahyadi1*, Edy Parwanto2, Hosea Jaya Edy3, Joey Joshua Vidova Tjahyadi4, Laurentia Gabrielle4, Ashaolu Victoria Oladimeji5, Seçil Karahüseyin6
1Department of Histology, Faculty of Medicine, Universitas Trisakti, Indonesia.
2Department of Biology, Faculty of Medicine, Universitas Trisakti, Indonesia.
3Study Program of Pharmacy, Faculty of Math, and Natural Sciences, Universitas Sam Ratulangi, Indonesia.
4Medical Education Program, Faculty of Medicine, Universitas Trisakti, Indonesia.
5Department of Chemistry, Loyola Institute of Frontier Energy, Loyola college, Chennai, India.
6Department of Pharmacognosy, Faculty of Pharmacy, Çukurova Universitesi, Turkey.
*Corresponding Author
Published In:
Volume - 18,
Issue - 10,
Year - 2025
ABSTRACT:
Facts show that many Indonesians are smokers. Some of these smokers are kretek smokers. Kretek is made from a mixture of tobacco, cloves, and sauce. Kretek smoke is toxic, and affects the cerebellum, especially Purkinje cells. Aim of this study was to determine the effects of kretek smoke on the Purkinje cells of rats. CG (control group) were rats that breathed using normal air (not exposed to kretek smoke). TG (treatment group) were rats that were exposed to smoke of kretek 1 stick/day for 1 month (30 days). Observations were made on the morphology of the rat brain and histology on the cerebellum. Qualitative and quantitative observations were made on Purkinje cells (area, perimeter, length, and width). The nicotine content in the kretek cigarettes used in this study was 3.88±0.05µg/g. Exposure to kretek smoke of 1 stick/day for 1 month in rats demonstrated that the brain did not experience changes in shape, but Purkinje cells in the cerebellum became abnormal in both shape and size. Purkinje cells area of rats in CG was higher than TG (p = 0.000). Purkinje cells perimeter of rats in CG was higher than TG (p = 0.000). Purkinje cells length of rats in CG was higher than TG (p = 0.000). Purkinje cells width of rats in CG was higher than TG (p = 0.000). Exposure to kretek smoke of 1 stick/day for 1 month did not change the morphology of the rat brain, but reduced the size of Purkinje cell bodies, and changed the shape from round to tapered as a sign of cell degeneration.
Cite this article:
David Tjahyadi, Edy Parwanto, Hosea Jaya Edy, Joey Joshua Vidova Tjahyadi, Laurentia Gabrielle, Ashaolu Victoria Oladimeji, Seçil Karahüseyin. Histometric Analysis to Perform Purkinje cell Abnormalities in Rats Due to Low doses of Kretek Smoke Exposure. Research Journal of Pharmacy and Technology. 2025;18(10):4882-0. doi: 10.52711/0974-360X.2025.00704
Cite(Electronic):
David Tjahyadi, Edy Parwanto, Hosea Jaya Edy, Joey Joshua Vidova Tjahyadi, Laurentia Gabrielle, Ashaolu Victoria Oladimeji, Seçil Karahüseyin. Histometric Analysis to Perform Purkinje cell Abnormalities in Rats Due to Low doses of Kretek Smoke Exposure. Research Journal of Pharmacy and Technology. 2025;18(10):4882-0. doi: 10.52711/0974-360X.2025.00704 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-10-43
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