Author(s): Ifora Ifora, Sri Oktavia

Email(s): iforafo03@gmail.com , sri.oktavia889@gmail.com

DOI: 10.52711/0974-360X.2025.00701   

Address: Ifora Ifora, Sri Oktavia
Padang, Wast Sumatera, Indonesia.
*Corresponding Author

Published In:   Volume - 18,      Issue - 10,     Year - 2025


ABSTRACT:
Breast cancer remains a significant global health issue, driving the need for safer and more effective treatments. Recent efforts have focused on natural compounds as potential adjuvants to improve chemotherapy outcomes and reduce toxicity. The study aimed to evaluate the cytotoxic effects of Garcinia cowa leaf ethanol extract (GCLEE) and its potential to enhance doxorubicin's cytotoxic activity on MCF-7 breast cancer cells. The cytotoxic effects of GCLEE and Doxorubicin (DOX) were assessed using the MTT assay on MCF-7 breast cancer cells. The test results were examined using a microplate reader set to operate at 595 nm wavelength. Chou Talalay's method was utilised to compute and analyse the combination index (CI) in order to ascertain the influence of the combination. Based on the MTT assay, both GCLEE and DOX exhibited concentration-dependent cytotoxic effects with IC50 values of 6,12 µg/mL and 1,76 µg/mL on MCF-7 cells, respectively. GCLEE synergistically enhances the cytotoxic effect of DOX, with a CI value range of 0.30–0.89. The combination of GCLEE and doxorubicin presents a promising synergistic strategy against breast cancer, with the potential to improve treatment effectiveness while reducing the toxicity associated with conventional chemotherapy.


Cite this article:
Ifora Ifora, Sri Oktavia. Synergistic Cytotoxic effects of Garcinia cowa Leaf Extract and Doxorubicin on MCF-7 Breast Cancer Cells: A Promising Strategy for Enhanced Chemotherapy Efficacy. Research Journal of Pharmacy and Technology. 2025;18(10):4865-0. doi: 10.52711/0974-360X.2025.00701

Cite(Electronic):
Ifora Ifora, Sri Oktavia. Synergistic Cytotoxic effects of Garcinia cowa Leaf Extract and Doxorubicin on MCF-7 Breast Cancer Cells: A Promising Strategy for Enhanced Chemotherapy Efficacy. Research Journal of Pharmacy and Technology. 2025;18(10):4865-0. doi: 10.52711/0974-360X.2025.00701   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-10-40


REFERENCES: 
1.    Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 Countries. CA Cancer J Clin. 2021; 71(3): 209–49. doi: 10.3322/caac.21660
2.    Dange VN, Shid SJ, Magdum CS, Mohite SK. A Review on Breast cancer: An Overview. Asian J Pharm Res. 2017; 7(1): 49–51. doi: 10.5958/2231-5691.2017.00008.9  
3.    Sun YS, Zhao Z, Yang ZN, Xu F, Lu HJ, Zhu ZY, Shi W, Jiang J, Yao PP, Zhu, HP. Risk factors and preventions of breast cancer. Int J Biol Sci. 2017; 13(11): 1387–97. doi: 10.7150/ijbs.21635
4.    Globocan. Cancer Incident in Indonesia. Int agency Res cancer . 2020; 858: 1–2. 
5.    Kaur S, Mayanglambam P, Bajwan D, Thakur N. Chemotherapy and its Adverse Effects – A Systematic Review. Int J Nurs Educ Res. 2022; 10(4): 399–402. doi: 10.52711/2454-2660.2022.00090 
6.    Sen A, Kumar K, Khan S, Pathak P, Singh A. Current Therapy in Cancer: Advances, Challenges, and Future Directions. Asian J Nurs Educ Res. 2024; 14(1): 77–84. doi: 10.52711/2349-2996.2024.00016
7.    Kumar A, Singh K, Kumar K, Singh A, Tripathi A, Tiwari L. Drug Resistance in Cancer Therapy: Mechanisms, Challenges and Strategies. Asian J Nurs Educ Res. 2024; 14(1): 95–100. doi: 10.52711/2349-2996.2024.00019
8.    Rawat PS, Jaiswal A, Khurana A, Bhatti JS, Navik U. Doxorubicin-induced cardiotoxicity: An update on the molecular mechanism and novel therapeutic strategies for effective management. Biomed Pharmacother. 2021; 139: 111708. doi: 10.1016/j.biopha.2021.111708
9.    Jishala MI, Venkateswaramurthy N, Sambathkumar R. Cardiotoxicity associated with cancer chemotherapy. Res J Pharm Technol. 2020; 13(5): 2480–7. doi: 10.5958/0974-360X.2020.00443.6
10.    Dali R, Hamama Z, Abdullah N. Study of Short-term toxicity of Cyclophosphamide and Doxorubicin on the Cardiovascular System in women with Breast cancer treated at the center of radiation and chemotherapy at Tishreen University Hospital. Res J Pharm Technol. 2022; 15(8): 3429–33. doi: 10.52711/0974-360X.2022.00574
11.    Perkins DW, Steiner I, Haider S, Robertson D, Buus R, O’Leary L, Isacke CM. Therapy-induced normal tissue damage promotes breast cancer metastasis. Science. 2024; 27(1): 108503. doi: 10.1016/j.isci.2023.108503.
12.    Jaibu AE, Shanmuga Sundaram R, Krishnaveni K, Sambathkumar R. Targeted cancer therapy: Promises and reality. Res J Pharm Technol. 2018; 11(4): 1407–12. doi: 10.1186/s12943-023-01865-0
13.    Navya PN, Kaphle A, Srinivas SP, Bhargava SK, Rotello VM, Daima HK. Current trends and challenges in cancer management and therapy using designer nanomaterials. Nano Converg. 2019; 6(1): 1–30. doi: 10.1186/s40580-019-0193-2
14.    Wahyuni, Diantini A, Ghozali M, Sahidin I. A Review of Current treatment for Triple-Negative Breast Cancer (TNBC). Res J Pharm Technol. 2022; 15(1): 409–18. doi: 10.52711/0974-360X.2022.00068
15.    Hanušová V, Boušová I, Skálová L. Possibilities to increase the effectiveness of doxorubicin in cancer cells killing. Drug Metab Rev. 2011; 43(4): 540–57. doi: 10.3109/03602532.2011.609174
16.    Pal R, Subhra Saha R, Dey S, Das S. A Review on the Role of some Cardioprotective Natural Resources in Mitigating Doxorubicin-Induced Cardiotoxicity. Res J Pharm Technol. 2024; 17(6):2933–42. doi: 10.52711/0974-360X.2024.00459
17.    Talib WH, Awajan D, Hamed RA, Azzam AO, Mahmod AI, Al-yasari IH. Combination Anticancer Therapies Using Selected Phytochemicals. Molecules. 2022; 27(5452): 1–50. doi: 10.3390/molecules27175452
18.    Witzel I, Müller V. Targeted Therapies in Breast Cancer: New Approaches and Old Challenges. Breast Care. 2015;10(3):157–8. doi: 10.1159/000431067
19.    Shaikh AM, Shrivastava B, Apte KG, Navale SD. Medicinal Plants as Potential Source of Anticancer Agents: A Review. J Pharmacogn Phytochem JPPB. 2016; 5(2): 291–5. 
20.    Ahmed HH, Aglan HA, Elsayed GH, Hafez HG, Eskander EF. Quercetin offers chemopreventive potential against breast cancer by targeting a network of signalling pathways. Res J Pharm Technol. 2021; 14(5): 2829–39. doi: 10.52711/0974-360X.2021.00499
21.    Ifora I, Oktaviani D, Fauziah F, Rahmaddian T. Anti-inflammatory Activity and Cyclooxygenase-2 Inhibitory effect of Peperomia pellucida (L.) Kunth Extract. Res J Pharm Technol. 2023; 16(8): 3727–32. doi: 10.52711/0974-360X.2023.00615
22.    Ifora I, Rahmaddian T. Antioxidant Potential of Garcinia cowa Roxb. A Review. 2022; 7(9): 20-26. doi: 10.47760/ijpsm.2022.v07i09.003
23.    Lim T. Cajanus cajan. In: Edible Medicinal And Non-Medicinal Plants. Springer, Dordrecht. Springer, Dordrecht. 2012. 549–568. 
24.    Lin F, Luo B, Cheng Z, Li P, Long C. Ethnobotanical Study on Garcinia (Clusiaceae) in China. Acta Soc Bot Pol. 2021; 90: 1-18. doi: 10.5586/asbp.9012
25.    Ritthiwigrom T, Laphookhieo S, Pyne SG. Chemical constituents and biological activities of Garcinia cowa Roxb. Maejo Int J Sci. Technol. 2013; 7(2): 212–31. 
26.    Hefni D, Dachriyanus, Wahyuni FS, Yerizel E, Arisanty D, Yusra LN. Cowanin, a Cytotoxic Xanthone from Asam Kandis (Garcinia cowa, Roxb.) Reduced Cell Migration and Induced Cell Cycle Arrest on T47D Human Cancer Cell. Int J Adv Sci Eng Inf Technol. 2020; 10(5): 2164–9. doi: 10.18517/ijaseit.10.5.12502
27.    Siridechakorn I, Maneerat W, Sripisut T, Ritthiwigrom T, Cheenpracha S, Laphookhieo S. Biphenyl and xanthone derivatives from the twigs of a Garcinia sp. (Clusiaceae). Phytochem Lett. 2014; 8(1): 77–80. doi: 10.1016/j.phytol.2014.02.004
28.    Wahyuni FS. Tetraprenyltoluquinone, an Anticancer Compound from Garcinia cowa Roxb Induce Cell Cycle Arrest. Int J Pharm Sci Rev Res Relat. 2015;166–8. 
29.    Husni E, Nahari F, Wirasti Y, Wahyuni FS, Dachriyanus. Cytotoxicity study of ethanol extract of the stem bark of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line. Asian Pac J Trop Biomed. 2015; 5(3): 249–52. doi: 10.1016/S2221-1691(15)30013-7
30.    Wahyuni FS, Triastuti DH, Arifin H. Cytotoxicity study of ethanol extract of the leaves of asam kandis (Garcinia cowa Roxb.) on T47D breast cancer cell line. Pharmacogn J. 2015; 7(6): 369–71. doi: 10.5530/pj.2015.6.9
31.    Abdulqawi LNA, Quadri SA, Islam S, Santra MK. Evaluation of Anticancer Activity of Withania somnifera L. and Tribulus terrestris L. on Human Breast Cancer Cells In vitro. Res J Pharm Technol. 2023; 16(7): 3079–82. doi: 10.52711/0974-360X.2023.00506
32.    Viswanathan N, Gowri V, Punnagai K. Investigation of In-vitro Antiproliferative activity of Ampelocissus latifolia root extract on MCF-7 breast cancer cells Ampelocissus latifolia root extract against MCF-7 breast cancer cells. Res J Pharm Technol. 2023; 16(8): 3595–600. doi: 10.52711/0974-360X.2023.00593
33.    Xia Z, Zhang H, Xu D, Lao Y, Fu W, Tan H, Cao P, Yang L, Xu H. Xanthones from the leaves of garcinia cowa induce cell cycle arrest, apoptosis, and autophagy in cancer cells. Molecules. 2015; 20(6): 11387–99. doi:10.3390/molecules200611387
34.    Nugroho AE, Hermawan A, Putri DDP, Novika A, Meiyanto E. Combinational effects of hexane insoluble fraction of Ficus septica Burm. F. and doxorubicin chemotherapy on T47D breast cancer cells. Asian Pac J Trop Biomed. 2013; 3(4): 297–302. doi: 10.1016/S2221-1691(13)60066-0
35.    Mujwar S, Kołat D, Kciuk M, Gieleci A, Kałuzi Z. Doxorubicin — An Agent with Multiple Mechanisms of Anticancer Activity. Cells. 2023; 12(659): 26–32. doi: 10.3390/ cells12040659
36.    Ashton JC. Drug combination studies and their synergy quantification using the chou-talalay method-letter. Cancer Res. 2015; 75(11): 2400. doi: 10.1158/0008-5472.CAN-14-3763
37.    Huang L, Jiang Y, Chen Y. Predicting Drug Combination Index and Simulating the Network-Regulation Dynamics by Mathematical Modeling of Drug-Targeted EGFR-ERK Signaling Pathway. Sci Rep. 2017; 7: 1–9. doi: 10.1038/srep40752
38.    Chou TC. Drug combination studies and their synergy quantification using the chou-talalay method. Cancer Res. 2010;70(2):440–6. doi: 10.1158/0008-5472.CAN-09-1947
39.    Boța M, Vlaia L, Jîjie AR, Marcovici I, Crişan F, Oancea C, Dehelean CA, Mateescu T, Moacă EA. Exploring Synergistic Interactions between Natural Compounds and Conventional Chemotherapeutic Drugs in Preclinical Models of Lung Cancer. Pharmaceuticals. 2024; 17(5): 1–36. doi: 10.3390/ph17050598
40.    Pezzani R, Salehi B, Vitalini S, Iriti M, Zuñiga FA, Sharifi‐Rad J, Martorell M, Martins N. Synergistic effects of plant derivatives and conventional chemotherapeutic agents: An update on the cancer perspective. Med. 2019; 55(4): 1–16. doi: 10.3390/medicina55040110




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