Author(s): B Bavishya, Seemone Khadri, C K Ashiga, Bhavaneshwari, Nair Sreecha Chandran

Email(s): bavishyaaa@gmail.com , naazsuha43@gmail.com , ashuashiga@gmail.com , pallu19012004@gmail.com

DOI: 10.52711/0974-360X.2025.00688   

Address: B Bavishya, Seemone Khadri, C K Ashiga, Bhavaneshwari, Nair Sreecha Chandran*
B.Tech Biotechnology (Student), The Oxford College of Engineering, Bangalore, Karnataka.
*Corresponding Author

Published In:   Volume - 18,      Issue - 10,     Year - 2025


ABSTRACT:
Protease refers to enzymes that catalyze the peptide bond hydrolysis of proteins. Proteolytic enzymes has its presence in different forms of life like microorganisms, animals and plants. Among them, protease from microorganisms occupies a safe position, as they have functional characteristics and enormous industrial applications. Microbial proteases have broad uses in different industries like food, detergent, and leather in bioremediation. In the food industry, they are used in the modification, improved quality, and storage stability of protein sources and meat tenderization. The present study screened microorganisms from household wastewater for novel protease bacteria in a casein agar plate. Among the isolates, the colony with the highest zone of proteolytic activity was selected for further analysis, it was identified as Enterobacter ludwigii by molecular characterization through BLAST. The optimization was carried out for protease enzyme for pH, carbon, organic, and inorganic nitrogen sources, and incubation time determines the maximum protease production by A1 isolate were at pH 9 and 72 hours of incubation. Among different parameters used in optimization, carbon source (Sucrose) showed maximum enzyme activity of 26.4 U. It can be used for further application studies.


Cite this article:
B Bavishya, Seemone Khadri, C K Ashiga, Bhavaneshwari, Nair Sreecha Chandran. Molecular Characterization of Protease Enzyme and its Optimization Study. Research Journal of Pharmacy and Technology. 2025;18(10):4781-4. doi: 10.52711/0974-360X.2025.00688

Cite(Electronic):
B Bavishya, Seemone Khadri, C K Ashiga, Bhavaneshwari, Nair Sreecha Chandran. Molecular Characterization of Protease Enzyme and its Optimization Study. Research Journal of Pharmacy and Technology. 2025;18(10):4781-4. doi: 10.52711/0974-360X.2025.00688   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-10-27


REFERENCE: 
1.    Higa, T., and Parr, J. F. Beneficial and effective microorganisms for sustainable agriculture and environment (Vol. 1). Essays in Biochemistry. 1994; 69: 1. doi.org/10.1093/aibsbulletin/6.2.35-h
2.    Robinson, P. K. Enzymes: principles and biotechnological applications. Essays in Biochemistry. 2015; 59: 1. doi.org/10.1093/aibsbulletin/6.2.35-h
3.    Hasan, F., Shah, A. A., and Hameed, A. Industrial applications of microbial proteases. Enzyme and Microbial Technology. 2006; 39(2): 235-251. doi.org/10.1016/j.enzmictec.2015.06.019.
4.    Solanki, P., Putatunda, C., Kumar, A., Bhatia, R., and Walia, A. Microbial  proteases: ubiquitous enzymes with innumerable uses. 3 Biotech. 2021; 11(10): 1-25. doi.org/10.1007/s13205-013-0149-x
5.    Razzaq, A., Shamsi, S., Ali, A., Ali, Q., Sajjad, M., Malik, A., and Ashraf, M. Microbial proteases applications. Frontiers in Bioengineering and Biotechnology. 2019; 7: 110. doi.org/10.3233/BME-130776
6.    Sharma, K. M., Kumar, R., Panwar, S., and Kumar, A. Microbial alkaline proteases: Optimization of production parameters and their properties. Journal of Genetic Engineering and Biotechnology. 2017; 15(1): 115-126. doi.org/10.1016/j.jgeb.2024.100451
7.    Nasri, M. Protein hydrolysates and biopeptides: Production, biological activities, and applications in foods and health benefits. A review. Advances in food and Nutrition Research. 2017; 81:, 109-159. doi.org/10.1016/B978-0-12-416555-7.09987-2.
8.    Oumer, O. J., and Abate, D. Screening and molecular identification of pectinase-producing microbes from the coffee pulp. BioMed Research International. 2018; 2018. doi.org/10.17605/OSF.IO/3P2HY
9.    Raj, A., Khess, N., Pujari, N., Bhattacharya, S., Das, A., and Rajan, S. S. Enhancement of protease production by Pseudomonas aeruginosa isolated from dairy effluent sludge and determination of its fibrinolytic potential. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(3): S1845-S1851. doi.org/10.1016/j.apjtb.2017.07.004.
10.    Sathishkumar, R., Ananthan, G., and Arun, J. Production, purification and characterization of alkaline protease by ascidian associated Bacillus subtilis GA CAS8 using agricultural wastes. Biocatalysis and Agricultural Biotechnology. 2015; 4(2): 214-220. doi.org/10.1016/j.bcab.2023.102617
11.    Rozanov, A. S., Shekhovtsov, S. V., Bogacheva, N. V., Pershina, E. G., Ryapolova, A. V., Bytyak, D. S., and Peltek, S. E. Production of subtilisin proteases in bacteria and yeast. Vavilov Journal of Genetics and Breeding. 2021; 25(1): 125. doi.org/10.17221/66/2024-CJGPB.


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