Author(s): Saida Hanoun, Hadjer Belkoum, Wiam Ouchene, Kelthoum Ahmed Gaid, Houssem Chenna, Hanane Mellal, Khaoula Aroua

Email(s): saida.hanoun@univ-khenchela.dz , hanoun298@gmail.com

DOI: 10.52711/0974-360X.2025.00053   

Address: Saida Hanoun1*, Hadjer Belkoum1, Wiam Ouchene1, Kelthoum Ahmed Gaid2, Houssem Chenna3, Hanane Mellal1, Khaoula Aroua4
1Department of Molecular and Cellular Biology, Faculty of Natural and life Sciences, University of Abbes Laghrour, 40004, Khenchela, Algeria.
2Department of Natural and Life Sciences, Abdel Hafid Boussouf University Center, 43000, Mila, Algeria.
3Pharmaceutical Sciences Research Center (CRSP), Constantine, 25000, Algeria.
4Department of Agronomy, Faculty of Natural and Life Sciences, University of Abbes Laghrour, 40004, Khenchela, Algeria.
*Corresponding Author

Published In:   Volume - 18,      Issue - 1,     Year - 2025


ABSTRACT:
This study aimed to evaluate in-vitro the antibacterial activity of Polygonum aviculare and Zygophyllum album extracts against multidrug-resistant Gram-negative bacteria (MDR-GNB) and to determine their total phenol content (TPC) and total flavonoid content (TFC). The effect of methanol and ethanol plant extracts was investigated against 3 Gram-negative reference strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603) and four clinical MDR-GNB strains (Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli 1 and Escherichia coli 2) by disc diffusion using 2 concentrations (100 and 200 mg/mL). The results revealed that P. aviculare extracts (methanol or ethanol) exhibited significant activity against different MDR-GNB strains with inhibitory zones ranging from 9 to 19 mm, while Z. album extracts showed antibacterial activity against E. coli 1, P. aeuginosa, E. coli ATCC 25922 and K. pneumoniae ATCC 700603 with inhibitory zones varying between 9 to 10.66 mm. The TFC values of plants extracts showed that the highest phenolic contents were found in methanol and ethanol extracts of P. aviculare (161.99±11.93 and 158.73±8.36µg GAE/mg respectively). Whereas both Z. album methanol and ethanol extracts contained considerably lower content of phenols (19.55±1.79 and 25.40±2.39µg GAE/mg). The TFC values also showed similar trends with that of TPC values. The highest TFC values were obtained for P. aviculare extracts (30.87±3.76µg QE/mg for methanol extract and 46.43±5.82µg QE/mg for ethanol extract), and the lowest were for Z. album extracts (19.14±6.11 for methanol extract and 21.40±0.98 µg QE/mg for ethanol extract).


Cite this article:
Saida Hanoun, Hadjer Belkoum, Wiam Ouchene, Kelthoum Ahmed Gaid, Houssem Chenna, Hanane Mellal, Khaoula Aroua. Total Phenol Content, Total Flavonoid Content and, In vitro, Antibacterial Activity of Polygonum aviculare and Zygophyllum album Extracts from Algeria against Multidrug-resistant Gram-negative Bacteria (MDR-GNB). Research Journal of Pharmacy and Technology. 2025;18(1):339-4. doi: 10.52711/0974-360X.2025.00053

Cite(Electronic):
Saida Hanoun, Hadjer Belkoum, Wiam Ouchene, Kelthoum Ahmed Gaid, Houssem Chenna, Hanane Mellal, Khaoula Aroua. Total Phenol Content, Total Flavonoid Content and, In vitro, Antibacterial Activity of Polygonum aviculare and Zygophyllum album Extracts from Algeria against Multidrug-resistant Gram-negative Bacteria (MDR-GNB). Research Journal of Pharmacy and Technology. 2025;18(1):339-4. doi: 10.52711/0974-360X.2025.00053   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-1-53


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