Author(s):
Nur Ariska Nugrahani, Retno Pudji Rahayu, Muhammad Mulky Sulaiman
Email(s):
a-retno-p-r@fkg.unair.ac.id
DOI:
10.52711/0974-360X.2025.00043 
Address:
Nur Ariska Nugrahani1, Retno Pudji Rahayu2*, Muhammad Mulky Sulaiman3
1Faculty of Dentistry, Universitas Muhammadiyah Surakarta, Surakarta, Central Java, 57141, Indonesia.
2Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Airlangga University, East Java, 60131, Indonesia.
3Postgraduate Immunology, Airlangga University, East Java, 60131, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 1,
Year - 2025
ABSTRACT:
A flare-up may happen in the root canal that has undergone endodontic treatment because the root canal becomes inflamed even after the treatment and causes the patients to feel severe pain and swelling in the affected tissue site. Enterococcus faecalis bacteria is a gram-positive and facultative anaerobes bacterium that can multiply to form colonies and form biofilm to protect themselves from the immune system of the host they infected to prevent them from being annihilated also protect them from anti-microbial drugs that may prolong the inflammation pathways. This study analyzes the expression of MAPK p38, Nf-?b p65, TNF alpha, and TLR2. All groups were prepared for molar cavitation using a round bur until the root canal and then injected with E. faecalis bacteria with a concentration of 100%. They were observed for 24 hours, 72 hours, and 120 hours after injection. After that, the tooth was decalcified using 10% EDTA at 37°C, which took up to two months. The slicing then underwent preparations and an IHC examination was performed for the expression MAPK p38, Nf-?b p65, TNF alpha, and TLR2. Significant results (p<0.05) were found in MAPK p38 and TLR2 between the control with 24, 72, and 120 hours groups, also insignificant results (p>0.05) were found in Nf-kb p65 and TNF alpha 72 with 120 hours group. Enterococcus faecalis biofilm which is mediated by LTA only lasts for 24 hours through the MAPK p38 and TLR-2 pathways which can cause inflammation with increased TNF-alpha. At 72 hours to 120 hours, the biofilm of Enterococcus faecalis had decreased so that the pathway was less stable and showed no significance from the NFkb pathway which resulted in a decrease in TNF alpha.
Cite this article:
Nur Ariska Nugrahani, Retno Pudji Rahayu, Muhammad Mulky Sulaiman. The Mechanism of p38-MAPK (Mitogen-Activated Protein Kinase) Pathway Activation Mediated by Enterococcus faecalis in Root Canal Infection. Research Journal of Pharmacy and Technology. 2025;18(1):280-4. doi: 10.52711/0974-360X.2025.00043
Cite(Electronic):
Nur Ariska Nugrahani, Retno Pudji Rahayu, Muhammad Mulky Sulaiman. The Mechanism of p38-MAPK (Mitogen-Activated Protein Kinase) Pathway Activation Mediated by Enterococcus faecalis in Root Canal Infection. Research Journal of Pharmacy and Technology. 2025;18(1):280-4. doi: 10.52711/0974-360X.2025.00043 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-1-43
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