Author(s): Zhunussova Maira Abylovna, Ivasenko Svetlana Alexandrovna, Suleimen Yerlan, Sarsenbekova Akmaral Zhakhanovna, Abdullabekova Raisa Musulmanbekovna

Email(s): maira.zhunusova@mail.ru

DOI: 10.52711/0974-360X.2025.00037   

Address: Zhunussova Maira Abylovna1*, Ivasenko Svetlana Alexandrovna2, Suleimen Yerlan3, Sarsenbekova Akmaral Zhakhanovna4, Abdullabekova Raisa Musulmanbekovna5
1Associate Professor of the School of Pharmacy of the Karaganda Medical University (Karaganda, Kazakhstan),
2Professor of the School of Pharmacy of the Karaganda Medical University (40 Gogol Str., 100008
Karaganda, Kazakhstan)
3Professor of the Department of Chemistry, Chemical Technology and Ecology of the Kazakh University of Technology and Business (building 37 A., Kayim Mukhamedkhanov str., Yesil district, left bank, Astana, Kazakhstan)
4Associate Professor of the Departments of Physical and Analytical Chemistry of the Academician Ye.A. Buketov Karaganda State University, (28 Universitetskaya str., 100024, Karaganda, Kazakhstan)
5Professor of the School of Pharmacy of the Karaganda Medical University (40 Gogol Str., 100008
Karaganda, Kazakhstan)
*Corresponding Author

Published In:   Volume - 18,      Issue - 1,     Year - 2025


ABSTRACT:
Currently, obtaining medicines from wild plants is relevant since natural substances and medicines based on medicinal plants have a number of undeniable advantages over synthetic medicines. Some advantages include low toxicity and a wide range of therapeutic effects, as well as a low probability of side effects and complications, except in rare cases, because of their long-term use. Studies of the chemical composition and biological activity of isolated components from wild plants have revealed their practical use in medicine and revealed new opportunities for the development of new medicines based on renewable plant resources. a-Santonin was first discovered in plants of the Caprifoliaceae Juss. family. This article presents qualitative and quantitative analysis of the content of a-santonin in the aboveground parts of Scabiosa ochroleuca L., Lomelosia isetensis (L.) Soják and Dipsacus strigosus Willd. CO2-extracts, alcohol and an aqueous extracts from CO2-meal (waste from the production of carbon dioxide extract) of Scabiosa ochroleuca L.; CO2-extracts of Lomelosia isetensis (L.) Soják, Dipsacus strigosus Willd. ex Roem. & Schult. by GC/MS, HPLC, IR-spectroscopy and UV-spectrophotometry. On the chromatograms of the tested samples of the carbon dioxide extracts of the herb scabiosa pale yellow, iset scabiosa, yellow-flowered teasel, at 101.694, 101.489 and 101.43 minutes, a peak with M/z 246 appears which corresponds to the molecular ion of sesquiterpene lactone a-santonin. In the IR spectrum the presence of absorption bands in the area 1751.37,88.64 cm-1; 1735.94,73.84 cm-1, 1728.23, 65.7 indicates the presence of carbonyl lactone ring (C=O ?-lactone), and in the field 1465.91,94.33 cm-1; 1465.91,82.32 cm-1 exocyclic methylene group linked to the carbonyl ?-lactone. The ultraviolet spectrum of a sample of carbon dioxide extract from the herb Scabiosa ochroleuca L. in the region of 190 nm to 400 nm, it has an absorption maximum at a wavelength of 235 nm, which is characteristic of the exocyclic methylene group conjugated to the y-lactone carbonyl. Preparative high-performance liquid chromatography (HPLC) revealed that, the retention time of a-santonin was 12.00±0.3 min, which confirmed the presence of a-santonin in the studied extracts by comparing the retention time of the peak of a-santonin in the standard sample.


Cite this article:
Zhunussova Maira Abylovna, Ivasenko Svetlana Alexandrovna, Suleimen Yerlan, Sarsenbekova Akmaral Zhakhanovna, Abdullabekova Raisa Musulmanbekovna. Qualitative and Quantitative Determination of α-santonin in the Aboveground parts of plants of the Caprifoliaceae Juss. family. Research Journal of Pharmacy and Technology. 2025;18(1):239-4. doi: 10.52711/0974-360X.2025.00037

Cite(Electronic):
Zhunussova Maira Abylovna, Ivasenko Svetlana Alexandrovna, Suleimen Yerlan, Sarsenbekova Akmaral Zhakhanovna, Abdullabekova Raisa Musulmanbekovna. Qualitative and Quantitative Determination of α-santonin in the Aboveground parts of plants of the Caprifoliaceae Juss. family. Research Journal of Pharmacy and Technology. 2025;18(1):239-4. doi: 10.52711/0974-360X.2025.00037   Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-1-37


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