Author(s):
Sri Wahdaningsih, Shoma Rizkifani, Eka Kartika Untari, Intan Yap,
Email(s):
sriwahdaningsih.apt@pharm.untan.ac.id
DOI:
10.52711/0974-360X.2025.00010
Address:
Sri Wahdaningsih*, Shoma Rizkifani, Eka Kartika Untari, Intan Yap,
Jurusan Farmasi, Fakultas Kedokteran, Universitas Tanjungpura, Pontianak 78124 Kalimantan Barat, Indonesia.
*Corresponding Author
Published In:
Volume - 18,
Issue - 1,
Year - 2025
ABSTRACT:
The peel of the red dragon fruit (Hylocereus polyrhizus) contains abundant amounts of carotenoids, flavonoids, anthocyanins, and phenolic compounds—all of which are naturally occurring antioxidants. Strong flavonoid antioxidant quercetin protects against tissue damage brought on by a variety of medication toxicities. The aim of the present work was to determine the quantitiy of quercetin in red dragon fruit peel methanol extract using high-performance thin-layer chromatography (HPTLC) densitometric analysis technique. Quercetin levels were first qualitatively analysed using thin layer chromatography (KLT) and then quantitatively analyzed using HPTLC. HPTLC was performed on Xbridge C18 5µm, 4.6x250mm HPTLC plates with phosphoric acid 0,5%: methanol (60:40) as the solvent system. Quantitative analysis using TLC showed that the extract was positive for quercetin with rf value = 0.26. The total quercetin content obtained was 206.76ug/g. Based on the analysis results, it can be concluded that HPTLC is an accurate and sensitive method for separating and quantifying quercetin in methanol extract of dragon fruit peel. The results of this analysis can be used in the further development of traditional medicine.
Cite this article:
Sri Wahdaningsih, Shoma Rizkifani, Eka Kartika Untari, Intan Yap, Qualitative and Quantitative Analysis of Quercetin from Hylocereus polyrhizus Peel Methanol Extract by High-performance Thin Layer Chromatography. Research Journal of Pharmacy and Technology. 2025;18(1):63-6. doi: 10.52711/0974-360X.2025.00010
Cite(Electronic):
Sri Wahdaningsih, Shoma Rizkifani, Eka Kartika Untari, Intan Yap, Qualitative and Quantitative Analysis of Quercetin from Hylocereus polyrhizus Peel Methanol Extract by High-performance Thin Layer Chromatography. Research Journal of Pharmacy and Technology. 2025;18(1):63-6. doi: 10.52711/0974-360X.2025.00010 Available on: https://rjptonline.org/AbstractView.aspx?PID=2025-18-1-10
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