Author(s): Mursal Mursal, Nurdin Saidi, Mustanir Yahya, Murniana Murniana, and Binawati Ginting

Email(s): binawati@unsyiah.ac.id

DOI: 10.52711/0974-360X.2024.00689   

Address: Mursal Mursal, Nurdin Saidi, Mustanir Yahya, Murniana Murniana, and Binawati Ginting
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 9,     Year - 2024


ABSTRACT:
Exogenous antioxidants are required to attenuate the increased oxidative stress level which is responsible for the development of various diseases. Owing to this crucial role of antioxidants, chemists have explored plant-based antioxidant sources. This research aimed to investigate the antioxidant activity of Garcinia mangostana stem barks in-vitro. Herein, we extracted the stem barks of G. magostana L. using ethyl acetate solvent and subsequently performed the fractionation using column chromatography with a gradient elution system. We found that the extract was rich in flavonoids and other antioxidant-related compounds. Some samples from fractionation had higher antioxidant activity as compared with the whole extract (median inhibitory concentration, IC50 = 11.20 - 13.42 mg/L versus 16.81mg/L). Thin-layer chromatogram of the fractions revealed their richness in flavonoids and tannins. We conclude that G. magostana L. stem bark is a good source of antioxidants.


Cite this article:
Mursal Mursal, Nurdin Saidi, Mustanir Yahya, Murniana Murniana, and Binawati Ginting. Antioxidant profile of Ethyl Acetate extract from Garcinia mangostana L. stem barks and its Fractions: Investigation using DPPH inhibition assay. Research Journal of Pharmacy and Technology. 2024; 17(9):4461-4. doi: 10.52711/0974-360X.2024.00689

Cite(Electronic):
Mursal Mursal, Nurdin Saidi, Mustanir Yahya, Murniana Murniana, and Binawati Ginting. Antioxidant profile of Ethyl Acetate extract from Garcinia mangostana L. stem barks and its Fractions: Investigation using DPPH inhibition assay. Research Journal of Pharmacy and Technology. 2024; 17(9):4461-4. doi: 10.52711/0974-360X.2024.00689   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-9-50


REFERENCE:
1.    Rao A. Bharani M. Pallavi V. Role of antioxidants and free radicals in health and disease. Adv Pharmacol Toxicol. 2006; 7(1): 29-38.
2.    Stefanis L.  Burke R.E. Greene L.A. Apoptosis in neurodegenerative disorders. Current Opinion in Neurology. 1997; 10(4): 299-305. doi.org/
3.    Sas K. Robotka H. Toldi J. Vécsei L. Mitochondria, metabolic disturbances, oxidative stress and the kynurenine system, with focus on neurodegenerative disorders. Journal of the Neurological Sciences. 2007; 257(1-2): 221-239. doi.org/
4.    Zarkovic N. Roles and Functions of ROS and RNS in Cellular Physiology and Pathology. MDPI: 2020; 9: 767.
5.    Herb, M.; Schramm, M., Functions of ROS in Macrophages and Antimicrobial Immunity. Antioxidants. 2021; 10(2): 313.
6.    Campbell, E. L.; Colgan, S. P., Control and dysregulation of redox signalling in the gastrointestinal tract. Nature Reviews Gastroenterology and Hepatology. 2019; 16(2): 106-120.
7.    Flieger, J.;  Flieger, W.;  Baj, J.; Maciejewski, R., Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles. Materials. 2021; 14(15): 4135.
8.    Rajendran, R.;  Hemachander, R.;  Ezhilarasan, T.;  Keerthana, C.;  Saroja, D.;  Saichand, K.; Abdullah, M. G., Phytochemical analysis and in-vitro antioxidant activity of Mimosa pudica Lin. leaves. Research J Pharm and Tech. 2010; 3(2): 551-555.
9.    Prabha, S.;  Rao, M.; Kumar, M., Evaluation of in vitro Antioxidant, Antibacterial and Anticancer activities of leaf extracts of Cleome rutidosperma. Research Journal of Pharmacy and Technology. 2017; 10(8): 2492-2496.
10.    Vaghela, J.; Sisodia, S., In vitro antioxidant activity of Terminalia chebula fruit extracts. Research Journal of Pharmacy and Technology. 2011; 4 (12): 1835-1843.
11.    Sujatha, J.;  Asokan, S.; Rajeshkumar, S., Phytochemical analysis and antioxidant activity of chloroform extract of Cassis alata. Research Journal of Pharmacy and Technology. 2018; 11 (2): 439-444.
12.    Shankar, M.; Suthakaran, R., In-Vitro Antioxidant Activities of Various Extracts of Eugenia jambolana Leaves. Research Journal of Pharmacy and Technology. 2014; 7(12): 3.
13.    Bagali, R. S.;  Jalalpure, S. S.; Patil, S., Evaluation of Schreberas wietenioides Roxb. fruit Ethanolic extract for Antioxidant and Hepatoprotective activity against CCl4 induced liver injury in rats. Research J. Pharm. and Tech. 2020; 13(11): 5115-5120.
14.    Byahatti, V.;  Khan, A.;  Patil, S.; Choudhury, A., Preliminary Phytochemical Investigation and In-vitro Antioxidant Activity of Bergenia ciliata Leaves. Research J. Pharm. and Tech. 2010; 3(2): 399-401.
15.    Nanditha, R.;  Saravanan, J.;  Praveen, T.;  Deepa, S.; Rymbai, E., Evaluation of anti-cancer, antioxidant and antimicrobial activities of Alstoniascholaris L. Research Journal of Pharmacy and Technology. 2020; 13(9): 4153-4157.
16.    Singh, S.;  Dhande, S. R.;  Aggarwal, S. M.;  Suryawanshi, A.; Kadam, V., In vitro Antioxidant Activity of 70% Methanolic Extracts of Roots of Hemidesmus indicus. Research Journal of Pharmacy and Technology. 2012; 5(9): 1241-1245.
17.    Gautam, V.;  Kapoor, D.;  Arora, S.; Bhardwaj, R., Characterization and Antioxidant Activity of oil Extract of a Gymnosperm-Araucaria cunninghamiiAiton ex D. Don. Research Journal of Pharmacy and Technology. 2016; 9(7): 875-879.
18.    Praveen, D.;  Chowdary, P. R.;  Thanmayi, G.;  Poojitha, G.; Aanandhi, M. V., Antioxidant and analgesic activity of leaf extracts of Artocarpus heterophyllus. Research Journal of Pharmacy and Technology. 2016; 9(3): 257-261.
19.    Azlim Almey, A.;  Ahmed Jalal Khan, C.; Syed Zahir, I.; Mustapha Suleiman, K.; Aisyah, M.; Kamarul Rahim, K., Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants' leaves. International Food Research Journal. 2010; 17(4).
20.    Hussain, A. K., T. Sehar, S. Sarwar, A. Ashraf, A. H. Jamil, M. A. Noreen, S. Rafique, A. Iftikhar, K. Aslam, J. Quddoos, M. Y. Majeed, M. A. Zerlasht, M., Utilization of pumpkin, pumpkin powders, extracts, isolates, purified bioactives and pumpkin based functional food products: A key strategy to improve health in current post COVID 19 period: An updated review. Applied Food Research. 2022; 2(2).
21.    Zhang, Y.; DeWitt, D. L.; Murugesan, S.; Nair, M. G., Novel lipid‐peroxidation‐and cyclooxygenase‐inhibitory tannins from Picrorhizakurroa seeds. Chemistry and Biodiversity. 2004; 1(3): 426-441.
22.    Lopes, G. K.; Schulman, H. M.; Hermes-Lima, M., Polyphenol tannic acid inhibits hydroxyl radical formation from Fenton reaction by complexing ferrous ions. Biochimica et Biophysica Acta (BBA)-General Subjects. 1999; 1472(1-2): 142-152.
23.    Tjahjani, S.; Widowati, W.; Khiong, K.; Suhendra, A.; Tjokropranoto, R., Antioxidant properties of Garcinia mangostana L (mangosteen) rind. Procedia Chemistry. 2014; 13: 198-203.
24.    Jung, H.A.; Su, B.N.; Keller, W. J.; Mehta, R. G.; Kinghorn, A. D., Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen). Journal of Agricultural and Food Chemistry. 2006; 54(6): 2077-2082.
25.    Yu, L.; Zhao, M.; Yang, B.; Zhao, Q.; Jiang, Y., Phenolics from hull of Garcinia mangostana fruit and their antioxidant activities. Food Chemistry. 2007; 104(1): 176-181.
26.    Yahya, M.; Ginting, B.; Saidi, N., In-Vitro screenings for biological and antioxidant activities of water extract from Theobroma cacao L. pod husk: Potential utilization in foods. Molecules. 2021; 26(22): 6915.
27.    Rizaldy, D.; Hartati, R.; Nadhifa, T.; Fidrianny, I., Chemical Compounds and Pharmacological Activities of Mangosteen (Garcinia mangostana L.)- Updated Review. Biointerface Res. Appl. Chem. 2021; 12: 2503-2516.
28.    Manimekalai, I.; Sivakumari, K.; Ashok, K.; Rajesh, S., Phytochemical profiling of mangosteen fruit, Garcinia mangostana. World J Pharm Pharm Sci. 2016; 5(2): 221-52.
29.    Obolskiy, D.; Pischel, I.; Siriwatanametanon, N.; Heinrich, M., Garcinia mangostana L.: a phytochemical and pharmacological review. Phytotherapy Research. 2009; 23(8): 1047-1065.
30.    Ginting, B.; Mustanir, M.; Nurdin, N.; Maulidna, M.; Murniana, M.; Safrina, S., Evaluation of antioxidant and anticancer activity of Myristica fragrans Houtt. bark. Pharmacognosy Journal. 2021; 13(3): 780-786.
31.    Ginting, B.; Saidi, N.; Murniana; Mustanir; Maulidna; Simanjuntak, P., Lignan compound isolated from n-Hexane extract Myristica fragrans Houtt root as antioxidant and antitumor activities against MCF-7 cell lines data. Data Brief. 2020; 31: 105997.
32.    Wang, Y. H.; Zhang, Y. R., Variations in compositions and antioxidant activities of essential oils from leaves of LuodianBlumea balsamifera from different harvest times in China. PLoS One. 2020; 15 (6): e0234661.
33.    Ginting, B.; Maulana, I.; Saidi, N.; Astryna, S. Y., Isolation and activity antioxidant test of cocoa pod husk ethyl asetat extracts (Theobroma cacao L). Jurnal Natural. 2019; 19(2): 49-53.
34.    Maulana, I.; Fasya, D.; Ginting, B., Biosynthesis of Cu nanoparticles using Polyalthia longifolia roots extracts for antibacterial, antioxidant and cytotoxicity applications. Materials Technology. 2022; 1-5.
35.    Mustanir, M.; Nurdin, N.; Ginting, B., Antioxidant Activity and Phytochemical Identification of Annona Squamosa Leaves Methanolic Extracts. Pharmacognosy Journal. 2021; 13(6s): 1746-1750.
36.    Elmund, B.; Hartrianti, P., Evaluation of mangosteen (Garcinia mangostana) antioxidant activity in clinical trials and in vivo animal studies: A systematic review. Journal of Applied Pharmaceutical Science. 2020; 10(12): 114–129.
37.    Tsai, S.Y.; Chung, P.C.; Owaga, E. E.; Tsai, I.; Wang, P.Y.; Tsai, J.I.; Yeh, T.S.; Hsieh, R.H., Alpha-mangostin from mangosteen (Garcinia mangostana Linn.) pericarp extract reduces high fat-diet induced hepatic steatosis in rats by regulating mitochondria function and apoptosis. Nutrition and Metabolism. 2016; 13(1): 1-10.
38.    Chang, C.C.; Chen, C.W.; Owaga, E.; Lee, W.T.; Liu, T.N.; Hsieh, R.H., Mangosteen concentrate drink supplementation promotes antioxidant status and lactate clearance in rats after exercise. Nutrients. 2020; 12(5): 1447.
39.    Karim, N.; Jeenduang, N.; Tangpong, J., Anti-glycemic and anti-hepatotoxic effects of mangosteen vinegar rind from Garcinia mangostana against HFD/STZ-induced type II diabetes in mice. Polish Journal of Food and Nutrition Sciences. 2018; 68(2).

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available