Author(s):
Priyanga Chandrasekaran, Shivashankar Kengadaran
Email(s):
shivashankar@igids.ac.in
DOI:
10.52711/0974-360X.2024.00688 
Address:
Priyanga Chandrasekaran1, Shivashankar Kengadaran2
1Department of Public Health Dentistry, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Pondicherry.
2Reader, Department of Public Health Dentistry, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth, Pondicherry.
*Corresponding Author
Published In:
Volume - 17,
Issue - 9,
Year - 2024
ABSTRACT:
Aim: To assess the antimicrobial activity of Alpinia officinale extract against S. mutans, lactobacillus, and C. albicans. Methodology: The rhizome of Alpinia officinale were identified, collected, washed, shade dried, and then ground to prepare a fine powder. 50gm of the prepared powder was dissolved in 250ml of the following solvents ethanol, acetone, ethyl acetate, chloroform and methanol at 40-50? for 6h. The bacterial and fungal isolates of S. mutans, Lactobacillus and C. albicans were collected and prepared. Antimicrobial and antifungal activity of extracts against S. mutans, lactobacillus and C. albicans were determined by the Disc diffusion method. Microdilution of extracts were prepared with 2X stock concentration to obtain 100 to 1.5 microgram per ml of nutrient broth to determine the minimum inhibitory concentration. Results: The most effective extract was ethanol extract followed by ethyl acetate and methanol extracts. There was substantial activity by methanol and ethyl acetate extracts. The MICs of different concentrations of ethanol extracts were found to be 1.5,3.1 and 12.5 µg/mL for S.mutans, L.acidophilus, and C.albicans respectively. Conclusion: The present study concluded that A. officinale has potential antimicrobial activities for cariogenic microorganisms.
Cite this article:
Priyanga Chandrasekaran, Shivashankar Kengadaran. Antimicrobial Efficacy of Alpinia officinale extracts against Oral Microorganism - An In-vitro Study. Research Journal of Pharmacy and Technology. 2024; 17(9):4457-0. doi: 10.52711/0974-360X.2024.00688
Cite(Electronic):
Priyanga Chandrasekaran, Shivashankar Kengadaran. Antimicrobial Efficacy of Alpinia officinale extracts against Oral Microorganism - An In-vitro Study. Research Journal of Pharmacy and Technology. 2024; 17(9):4457-0. doi: 10.52711/0974-360X.2024.00688 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-9-49
REFERENCES:
1. Miglani S et al. Burden of dental caries in India: Current scenario and future strategies. International Journal of Clinical Pediatric Dentistry. 2020; 13(2):10.5005/jp-journals-10005-1733
2. Mathur, V.P., Dhillon, J.K et al. Dental Caries: A Disease Which Needs Attention. Indian J Pediatr. 2018; 85(3): https://doi.org/10.1007/s12098-017-2381-6
3. Amirinia F, Pourhajibagher M et al. In vitro antimicrobial and cytotoxicity activities of some medicinal plant extracts against oral microbial pathogens. Folia Medica. 2021; 63(6): 10.3897/folmed.63.e56840
4. Regezi JA, Sciubba J, Jordan RC et al. Oral pathology: clinical pathologic correlations. Elsevier Health Sciences. 2016.
5. Sukkarwalla A, Ali SM, Lundberg P, Tanwir F et al. Efficacy of miswak on oral pathogens. Dent Res J. 2013; 10(3): 10.4103/1735-3327.115138
6. Clark AM et al. Natural products as a resource for new drugs. Pharmaceutical Research. 1996; 13. https://doi.org/10.1023/A:1016091631721
7. Srividya AR, Dhanabal SP, Misra VK, Suja G et al. Antioxidant and antimicrobial activity of Alpinia officinarum. Indian Journal of Pharmaceutical Sciences. 2010; 72(1): 10.4103/0250-474X.62233
8. Rao K, Ch B, Narasu LM, Giri A et al. Antibacterial activity of Alpinia galanga (L) Willd crude extracts. Applied Biochemistry and Biotechnology. 2010; 162(3). https://doi.org/10.1007/s12010-009-8900-9
9. Avci GA, Emre AV, Cilak GO, Cevher SC et al. Antimicrobial and Antioxidant Activities of Zingiber officinale Ginger and Alpinia officinarum Galangal. Hittite Journal of Science and Engineering. 2020; 7(1). https://doi.org/10.17350/HJSE19030000171
10. Anusha D, Chaly PE, Junaid M, Nijesh J E, Shivashankar K, Sivasamy S et al. Efficacy of a mouthwash containing essential oils and curcumin as an adjunct to nonsurgical periodontal therapy among rheumatoid arthritis patients with chronic periodontitis: A randomized controlled trial. Indian J Dent Res. 2019; 30(1). https://doi.org/10.17350/HJSE19030000171
11. Nirisha Sriram, Gheena. S, Samrithi Yuvaraj et al. Effects of turmeric on oral submucous fibrosis: A Systematic Review. Research J. Pharm. and Tech. 2015; 8(8): http://dx.doi.org/10.5958/0974-360X.2015.00180.8
12. S. Subasree, Karthikeyan Murthykumar, Dhanraj et al. Effect of Aloe Vera in Oral Health – A Review. Research J. Pharm. and Tech. 2016; 9(5): 10.5958/0974-360X.2016.00116.5
13. Niha Naveed, Karthikeyan Murthykumar, Subasree Soundarajan, Sripradha Srinivasan et al. The Use of Neem in Oral Health. Research J. Pharm. and Tech. 2014; 7(9).
14. Pearley Jesylne, Subasree Soundarajan, Karthikeyan Murthykumar, Meenakshi. M et al. The Role of Cardamom Oil in Oral Health: A Short Review. Research J. Pharm. and Tech. 2016; 9(3): 10.5958/0974-360X.2016.00050.0
15. S. Vignesh, Roy Anitha, S. Rajesh Kumar, T. Lakshmi et al. Evaluation of the Antimicrobial activity of Cumin oil mediated silver nanoparticles on Oral microbes. Research J. Pharm. and Tech. 2019; 12(8):10.5958/0974-360X.2019.00634.6
16. Aravind Kumar S., Lakshmi T., Arun A.V et al. In vitro Antibacterial Activity of Acacia catechu ethanolic leaf extract against selected acidogenic oral bacteria. Research J. Pharm. and Tech. 2012; 5(3)
17. Shivashankar Kengadaran, Srisakthi. D., Divvi Anusha, Gopinath Prakasam, Kavitha. M, Vigneshwari. S.K et al. Comparing the efficacy of Neem extract, Aloe vera juice, and 0.2% Chlorhexidine against dental unit waterline pathogens. an In-vitro study. Research Journal of Pharmacy and Technology. 2021; 14(4): 10.52711/0974-360X.2021.00395
18. Kengadaran S, Divvi A, Kumar P R, Gopinath P, Arumugham M I, Sakthi S D et al. Antimicrobial efficacy of herbal, homeopathic and conventional dentifrices against oral microflora: An in vitro study. Population Medicine. 2020; 2. https://doi.org/10.18332/popmed/122529
19. M. Vaishali et al. Popular Medicinal Plants used for Dental Diseases in India. Research J. Pharm. and Tech. 2014; 7(7)
20. Hindustan Abdul Ahad, Chinthaginjala Haranath, Sreenivasulu Kondaveeti, Srikanth Konjeti, Sreevidya Gangireddy, Omer Ibrahim et al. Herbs in dentifrices for dental care and hygiene. Research J. Pharm. and Tech. 2020; 13(10): 10.5958/0974-360X.2020.00885.9
21. Subhashree Singh, Bhaskar Chandra Sahoo, Subrat Kumar Kar, Ambika Sahoo, Sanghamitra Nayak, Basudeba Kar, Suprava Sahoo et al. Chemical constituents Analysis of Alpinia galanga and Alpinia calcarata. Research J. Pharm. and Tech. 2020; 13(10): 10.5958/0974-360X.2020.00834.3
22. Pillai MK, Ismail R, Sasidharan S, Asmawi MZ, Choon TS, Mekbib SB, Lachumy SJ et al. Antioxidant and antimicrobial activities of rhizome extracts from malaysian species of Alpinia galanga and Alpinia officinarum. Pharmacology Online. 2019; 1