Author(s):
Wahyuni, Fadhliyah Malik, Asniar Pascayantri, Adryan Fristiohady, Agung Wibawa Mahatva Yodha, Muhammad Ilyas Y, Asriullah Jabbar, Muhammad Hajrul Malaka, Sahidin, Wa Ode Sitti Musnina, Muhammad Nuzul Arkham
Email(s):
wahyuni@uho.ac.id
DOI:
10.52711/0974-360X.2024.00679 
Address:
Wahyuni1, Fadhliyah Malik1, Asniar Pascayantri1, Adryan Fristiohady1, Agung Wibawa Mahatva Yodha2, Muhammad Ilyas Y1,2, Asriullah Jabbar1, Muhammad Hajrul Malaka1, Sahidin1, Wa Ode Sitti Musnina3, Muhammad Nuzul Arkham1
1Department of Pharmacy, Faculty of Pharmacy, Halu Oleo¬University. Jl. HEA. Mokodompit, Anduonohu, Kendari, Southeast Sulawesi, Indonesia.
2Department of Pharmacy, Polytechnics of Bina Husada Kendari. Jl. Sorumba No. 17, Wua-Wua, Kendari, Southeast Sulawesi, Indonesia.
3Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Tadulako University.
*Corresponding Author
Published In:
Volume - 17,
Issue - 9,
Year - 2024
ABSTRACT:
Etlingera is a genus that has the potential as a medicinal plant. Etlingera alba (Blume) A.D. Poulsen is an Etlingera species discovered recently in Southeast Sulawesi. This research describes the isolation and discovery of secondary matabolites from the methanol extract of the stem of Etlingera alba (Blume) A.D. Poulsen and is activity test as an antioxidant. Compounds were isolated using chromatography techniques. Identification of the molecular structure of secondary metabolites was carried out bu spectroscopic techniques. The antioxidant test was carried out using the 1,1-dipheny1-2-picrylhydrazyl (DPPH) method. Three diarylheptanoids: yakuchinone A (1), 7-(4”-hydrozy-3”-methoxypheny-1- phenyl-hept-4-en-3-one (2), and oxyphyllacinol (3) have been isolated from the stems of E. alba. Based on the DPPH test, compound 1 showed very strong antioxidant activity with an IC50 value of 26.62µg/ml, wile compounds 2 and 3 showed strong activity with IC50 va;ies pf 73-72 and 63.2µg/ml respectively.
Cite this article:
Wahyuni, Fadhliyah Malik, Asniar Pascayantri, Adryan Fristiohady, Agung Wibawa Mahatva Yodha, Muhammad Ilyas Y, Asriullah Jabbar, Muhammad Hajrul Malaka, Sahidin, Wa Ode Sitti Musnina, Muhammad Nuzul Arkham. Three Antioxidant Diarylheptanoids from Etlingera alba Stems from Southeast Sulawesi. Research Journal of Pharmacy and Technology. 2024; 17(9):4394-0. doi: 10.52711/0974-360X.2024.00679
Cite(Electronic):
Wahyuni, Fadhliyah Malik, Asniar Pascayantri, Adryan Fristiohady, Agung Wibawa Mahatva Yodha, Muhammad Ilyas Y, Asriullah Jabbar, Muhammad Hajrul Malaka, Sahidin, Wa Ode Sitti Musnina, Muhammad Nuzul Arkham. Three Antioxidant Diarylheptanoids from Etlingera alba Stems from Southeast Sulawesi. Research Journal of Pharmacy and Technology. 2024; 17(9):4394-0. doi: 10.52711/0974-360X.2024.00679 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-9-40
REFERENCES:
1. Droop, J. Book review - Etlingera of Sulawesi. 2013. In: Poulsen, A.D. (Ed.), Edinburgh Journal of Botany, vol. 70. Nat Hist Pub (Borneo), Malaysia. 2012. DOI:10.1017/S096042861300019X£52.
2. Nagappan, T., Tatin, Y., and Skornickova, J. L. Investigation on bioactive potential of selected Wild Ginger, Genus Etlingera from Tasik Kenyir, Terengganu. Greater Kenyir Landscapes: Social Development and Environmental Sustainability: From Ridge to Reef. 2018; 75–82. https://doi.org/10.1007/978-3-319-92264-5_7
3. Tachai S, Nuntawong N. Uncommon secondary metabolites from Etlingera pavieana rhizomes. Nat Prod Res. 2016 Oct;30(19):2215-9. doi: 10.1080/14786419.2016.1146884. Epub 2016 Feb 22. PMID: 26901239.
4. Chan, Eric W.C., Lim, Y. Y., and Wong, S. K. Phytochemistry and pharmacological properties of Etlingera elatior: A review. Pharmacognosy Journal. 2011; 3(22), 6–10. https://doi.org/10.5530/pj.2011.22.2
5. Wahyuni, Grashella, S.H.L., Fitriah, W.O.I., Malaka M.H., Fristiohady A., Imran, and Sahidin, I. Antibacterial and antioxidant potentials of methanol extract and secondary metabolites from Wualae rhizome (Etlingera elatior). Current Research Bioscience and Biotechnology. 2019; 1(1), 13-16.
6. Fristiohady, A., Sadrun, B., Wahyuni, Yusuf, M.I., Malik, F., Purnama L.O.M.J., Bafadal, M., Leorita, M., Jabbar, A., and Sahidin, I. 2020. Hepatoprotective activity etlingera elatior (Jack) R.M. Smith extract against CCl4-induced hepatic toxicity in male wistar rats, Research Journal of Pharmacy and Technology. 2020; 13(10): 4585-4590.
7. Sahidin, I., Wahyuni, Malaka, M. H., Jabbar, A., Imran, and Manggau, M. A. Evaluation of antiradical scavenger activity of extract and compounds from Etlingera calophrys stems. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(2): 238–241. https://doi.org/10.22159/ajpcr.2018.v11i2.22535
8. Sahidin, I., Wahyuni, Rahim, A.R., Arba, M., Yodha, A.W.M., Rahmatika, N.S., Sabandar, C.W., Manggau, A.M., Khalid, R.M., Almuqarobbun, L.O.M.R., Rosandy, A.R., Chahyadi, A., Hartati, R., and Elfahmi. Radical scavenger and antimicrobe of diarylheptanoids and steroids from etlingera calophrysrhizome. Sustainable Chemistry and Pharmacy. 2022; 29: 100767.
9. Mahdavi, B. Chemical constituents of the aerial parts of Etlingera brevilabrum (Zingiberaceae). Der Pharma Chemica. 2014; 6(2): 360–365.
10. Hamsidi, R. Wahyuni, W., Sahidin, I., Apriyani, A., Harsono, H., Azizah, N. A., Malik, F., Yodha, A. W. M., Purnama, L. O. M. J., and Fristiohady, A. Suppression of proinflammatory cytokines by etlingera alba (A.D.) Poulsen rhizome extract and its antibacterial properties. Advances in Pharmacological and Pharmaceutical Sciences. 2021; 1-6
11. Wahyuni, Diantini, A., Ghozali, M., Subarnas, A., Julaeha, E., Amalia, R., and Sahidin, I. Phytochemical screening, toxicity activity and antioxidant capacity of ethanolic extract of etlingera alba rhizome. Pakistan Journal of Biological Sciences. 2021; 24(7): 807–814.
12. Wahyuni, Ajeng Diantini, Mohammad Ghozali, Anas Subarnas, Euis Julaeha, Riezki Amalia, Adryan Fristiohady, Andini Sundowo, Sofa Fajriah, Yuni Elsa Hadisaputri, Raden Maya Febrianti, Fauzia Azzahra, Agung W.M. Yodha, I. Sahidin.. In-Vitro Anticancer Activity Of Chemical Constituents From Etlingera Alba Poulsen Against Triple Negative Breast Cancer And In Silico Approaches Towards Matrix Metalloproteinase-1 Inhibition. Indonesian Journal of Science and Technology. 2022; 7(2): 2528-1410. https://doi.org/10.17509/ijost.v7i2.50547
13. S. Shanthi, M. Sahina Begum, M.Senthuja. In- vitro Antioxidant and Anti-aging activity of a Traditional Ayurvedic Formulation. Research Journal of Pharmacy and Technology. 2023; 16(8): 3521-4. doi: 10.52711/0974-360X.2023.00581
14. Pradeep Kumar Samal. Investigation of Antioxidant activity of Butea monosperm barks. Research J. Pharm. and Tech 6(6): June 2013; 610-613.
15. Gulzar Ahmed Rather, Anima Nanda, Ezekiel Raj, N. Mathivanan, K. Thiruvengadam, Mohmmad Ashaq Sofi, B. K. Nayak. Determination of Phytochemicals, in vitro Antioxidant and Antibacterial activity of Lavandula angustifolia Mill. Research Journal of Pharmacy and Technology. 2023; 16(3): 1161-6.
16. Ritu Paliwal, Veena Sharma, Pracheta, Sadhna Sharma. Elucidation of Free Radical Scavenging and Antioxidant Activity of Aqueous and Hydro-Ethanolic Extracts of Moringa oleifera Pods. Research J. Pharm. and Tech. 2011; 4(4): 566-571.
17. Jabbar A, Wahyuono S, Puspitasari I, Sahidin I. Free radical scavenging activity of methanol extract and compounds isolated from stems of Etlingera rubroloba A.D Poulsen. International Journal Of Pharmaceutical Research. 2021; 13(1): 1099-1105. doi:org/10.31838/ijpr/2021.13.01.478
18. Jabbar A, Hamzah H, Nandini E, et al. The Effectiveness of Begonia Multangula Blume Leaf Ethanol Extract as Polymicrobial Antibiofilm on Catheters. Egyptian Journal of Chemistry. 2022; 65(13). doi:10.21608/ejchem.2022.118622.5341
19. Y MI, Diantini A, Ghozali M, Sahidin I, Fristiohady A. Immunomodulatory Potency Etlingera rubroloba A.D. Poulsen Fruit Ethanol extract against Macrophage Phagocytic Activity and CD4 Levels in Wistar Male Rats. Research Journal of Pharmacy and Technology. 2022; 15(9): 4067-4072. doi:10.52711/0974-360X.2022.00682
20. Jabbar A, Wahyuono S, Puspitasari I, Sahidin I. Xanthine Oxidase Inhibitory Activity and DPPH radical scavenging Assay of isolated compound from Etlingera rubroloba (Blume) A.D Poulsen stem. International Journal of Pharmaceutical Research. 2021; 13(1): 1994-2002. doi:https://doi.org/10.3188/ijpr/2021.13.01.316
21. Jabbar A, Sahidin I, Monstavevi S, Malaka M, Malik F, Ilyas MY. Antioxidant and Anti-Inflammatory Activity of Ethanol Extract Stem of Etlingera rubroloba A.D. Poulsen. Pakistan Journal of Biological Sciences. 2022; 25(10): 885-891. doi:10.3923/pjbs.2022.885.891
22. Somanjana Khatua, Snigdha Paul, Krishnendu Acharya. Mushroom as the Potential Source of New Generation of Antioxidant: A Review. Research J. Pharm. and Tech. 2013; 6(5): May 496-505
23. Jabbar A, Hamzah H, Nandini E, et al. The Effectiveness of Begonia Multangula Blume Leaf Ethanol Extract as Polymicrobial Antibiofilm on Catheters. Egyptian Journal of Chemistry. 2022; 65(13). doi:10.21608/ejchem.2022.118622.5341
24. BP Muley, SS Khadabadi, NB Banarase, HA Sawarkar. The Antioxidant Activity of the Leaves and Petals of Calendula officinalis Linn. Research J. Pharm. and Tech. 2009; 2(1): 173-175.
25. Tiwari VK, Mishra BB. Opportunity, Challenge and Scope of Natural Products in Medicinal Chemistry. Research Signpost; 2011.
26. Wahyuni W, Yusuf MI, Malik F, Lubis AF, Indalifiany A, Sahidin I. Immunomodulatory Effects of Ethanol Extract of Melophlus sarasinorum Sponge Against Phagocytosis Activity of Macrophage Cells on Balb/C Male Mice. Galenika Journal of Pharmacy. 2019; 5(2): 147-157. doi:10.22487/j24428744.2019.v5.i2.13611
27. Chetan S. Sonawane, Sushama D. Patil, Deepali M. Jagdale, Leena Patil, Vilasrao J. Kadam. In Vitro Antioxidant Activity of Methanolic Extract of Leaves of Ornamental Plant Epipremnum aureum Linn. Research J. Pharm. and Tech. 2011; 4(8): 1237-1239.
28. Sandip Sutariya, Vidhi Pansuriya, Malika Sharma, AB Bajpai, Amit Gupta. Antimicrobial, Antioxidant and Anti-inflammatory potential of flavonoid and terpenoid from Gymnosporia montana. Research Journal of Pharmacy and Technology. 2023; 16(4):1945-0.
29. Solichah AI, Anwar K, Rohman A, Fakhrudin N. Phytochemical Profile and Antioxidant Activity of Some Artocarpus Genus Plants in Indonesia. Journal of Food and Pharmaceutical Sciences. Published online July 26, 2021:443-460. doi:10.22146/jfps.2026
30. Ning An, Hong-wu Zhang, Li-zhen Xu, Shi-lin Yang, dan Zhong-mei Zou. New diarylheptanoids from the rhizome of Alpinia officinarum Hance. Food Chemistry. 2010; 119 (2).
31. Gulzar Ahmed Rather, Anima Nanda, Ezekiel Raj, N. Mathivanan, K. Thiruvengadam, Mohmmad Ashaq Sofi, B. K. Nayak. Determination of Phytochemicals, in vitro Antioxidant and Antibacterial activity of Lavandula angustifolia Mill. Research Journal of Pharmacy and Technology. 2023; 16(3):1161-6.
32. Han, J., T., Sang Y., L., Youn H., L., Nam-In B, Antioxidative diarylheptanoids from the fruits of Alpinia oxyphylla, Food Science and Biotechnology. 2007; 16 (6).
33. Lin, R. J., Yen, C. M., Chou, T. H., Chiang, F. Y., Wang, G. H., Tseng, Y. P., Wang, L., Huang, T. W., Wang, H. C., Chan, L. P., Ding, H. Y., and Liang, C. H. Antioxidant, anti-adipocyte differentiation, antitumor activity and anthelmintic activities against Anisakis simplex and Hymenolepis nana of yakuchinone A from Alpinia oxyphylla. BMC Complementary and Alternative Medicine. 2013; 13. https://doi.org/10.1186/1472-6882-13-237
34. Wahyuni, Ajeng Diantini, Mohammad Ghozali, Sahidin. Etlingera Genus: Phytochemical Screening And Anticancer Activity. Jurnal Farmasi Sains dan Praktis. 2021; 7(3): 343-355
35. Yodha, A.W.M., Badia, E., Musdalipah, M., Setiawan, M. A., Daud, N. S., Fusvita, A., Fristiohady, A., and Sahidin, I. Essential Oils of Alpinia monopleura and Their Antibacterial and Antioxidant Activity. Molekul. 2023; 18(1): 80–88. https://doi.org/10.20884/1.jm.2023.18.1.6265
36. Imran, Wahyuni, Adryan Fristiohady, Mesi Leorita, M. Hajrul Malaka, Muhammad Ilyas Y, Musadar, Nur Syifa Rahmatika, Ahmad Darmawan, Sofa Fajriah, Agung W. Mahatva Yodha, Sahidin I. Radical Scavenger and Anti-diabetic Potencies of Etlingera elatior Fruits growing in South East Sulawesi-Indonesia. Research Journal of Pharmacy and Technology. 2022; 15(5): 2141-6. doi: 10.52711/0974-360X.2022.00355
37. Sandip Sutariya, Vidhi Pansuriya, Malika Sharma, AB Bajpai, Amit Gupta. Antimicrobial, Antioxidant and Anti-inflammatory potential of flavonoid and terpenoid from Gymnosporia montana. Research Journal of Pharmacy and Technology. 2023; 16(4): 1945-0.
38. Sumit Phulera, Neelam Gurung, Kavita Maria Arora, Gaurav Kumar, Loganathan Karthik, Kokati Venkata Bhaskara Rao. Evaluation of Phytochemical Composition, Antioxidant and Cytotoxic Activity of Ipomoea fistulosa Leaves (Convolvulaceae). Research J. Pharm. and Tech. 2014; 7(4): 454-459.