Author(s):
Deasy Ovi Harsachatri, Dian Nugrahenny, Setyawati Soeharto, Nur Permatasari
Email(s):
permatasarirazaq.fk@ub.ac.id
DOI:
10.52711/0974-360X.2024.00676 
Address:
Deasy Ovi Harsachatri1,2, Dian Nugrahenny3, Setyawati Soeharto3, Nur Permatasari3*
1Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia 65145.
2Study Program of Blood Bank Technology, Kartini Health Academy, Batam, Riau Islands, Indonesia 29483.
3Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia 65145.
*Corresponding Author
Published In:
Volume - 17,
Issue - 9,
Year - 2024
ABSTRACT:
Around 50% of women over 50 with menopause show osteoporosis symptoms, increasing fracture risk. Estrogen deficiency from menopause affects bone remodeling. Groundcherry (Physalis minima L.) has phytoestrogens. We studied groundcherry stem water extract (GSWE) on femur osteoblasts, osteoclasts, and malondialdehyde (MDA) in ovariectomized rats. Rats were grouped: sham-operated, 5-week ovariectomized, 9-week ovariectomized, and three GSWE-treated ovariectomized groups (500, 1500, and 2500mg/kgBW for four weeks). Hematoxylin-eosin staining quantified osteoblast and osteoclast numbers (1000x magnification) in right femur bones. Left femur bones were powdered for MDA analysis via thiobarbituric acid reactive substances (TBARS) test. For osteoblasts, 5-week (21.25±0.957) and 9-week ovariectomized rats (22.75±1.708) had reduced (p<0.01) osteoblast numbers compared to sham (33.75±2.754). GSWE-treated ovariectomized rats at 500 (46.00±1.414) and 1500 mg/kg BW (56.25±6.701) showed increased (p<0.0001) osteoblast numbers, but the 2500 mg/kg BW group had fewer osteoblast numbers (25.50±1.291), similar to the level seen in untreated ovariectomized rats (p>0.05). For osteoclasts, 5-week (25.00±0.816) and 9-week ovariectomized rats (29.50±1.732) had more (p<0.0001) osteoclast numbers compared to sham (17.75±1.893). Conversely, GSWE-treated ovariectomized rats showed fewer (p<0.01) osteoclast numbers at all doses than untreated ovariectomized rats. A significant negative correlation between GSWE doses and osteoclast numbers was observed (r=-0.595; p=0.015). MDA levels were higher (p<0.0001) in 9-week ovariectomized rats (0.115±0.020) than in sham (0.017±0.014). GSWE-treated ovariectomized rats had lower (p<0.0001) MDA levels at all doses than untreated ovariectomized rats. A negative correlation between GSWE doses and MDA levels was evident (r=-0.611; p=0.012). MDA levels correlated negatively with osteoblast numbers (r=-0.577; p=0.019) and positively with osteoclasts (r=0.790; p<0.0001). In conclusion, GSWE could potentially enhance bone health by elevating osteoblast numbers, reducing osteoclast numbers, and lowering MDA levels. These findings suggest its potential as a natural intervention for addressing menopause-related bone issues. Further research can explore its applications.
Cite this article:
Deasy Ovi Harsachatri, Dian Nugrahenny, Setyawati Soeharto, Nur Permatasari. Potential Benefits of Physalis minima Stem Water Extract on Bone Health in Ovariectomized Rats: Osteoblast, Osteoclast, and Malondialdehyde Modulation. Research Journal of Pharmacy and Technology. 2024; 17(9):4373-0. doi: 10.52711/0974-360X.2024.00676
Cite(Electronic):
Deasy Ovi Harsachatri, Dian Nugrahenny, Setyawati Soeharto, Nur Permatasari. Potential Benefits of Physalis minima Stem Water Extract on Bone Health in Ovariectomized Rats: Osteoblast, Osteoclast, and Malondialdehyde Modulation. Research Journal of Pharmacy and Technology. 2024; 17(9):4373-0. doi: 10.52711/0974-360X.2024.00676 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-9-37
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