Author(s):
Raed Abd-Elrahman Alhyasat, Khaled Khleifat, Ali M. Khlaifat, Ahmad Za’al Alsarayreh, Yaseen Taha Al Qaisi, Maha N. Abu Hajleh, Moath Alqaraleh, Alia K. Ibrahim
Email(s):
ahmsar@mutah.edu.jo
DOI:
10.52711/0974-360X.2024.00665 
Address:
Raed Abd-Elrahman Alhyasat1, Khaled Khleifat2, Ali M. Khlaifat3, Ahmad Za’al Alsarayreh2*, Yaseen Taha Al Qaisi2, Maha N. Abu Hajleh4, Moath Alqaraleh5, Alia K. Ibrahim6
1Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, Jordan.
2Department of Biological Sciences, Faculty of Science, Mutah University, P.O. Box 7, Mutah 61710, Jordan.
3Department of Nursing, Faculty of Prince Aysha for Applied Health and Nursing, Al-Hussein Bin Talal University, Ma’an, Jordan.
4Department of Cosmetic Science, Pharmacological and Diagnostic Research Centre, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Zip code (19328), Amman, Jordan.
5Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Al-Salt 19117, Jordan.
6Faculty of Medicine, Al-Balqa Applied University, Al-Salt, Jordan.
*Corresponding Author
Published In:
Volume - 17,
Issue - 9,
Year - 2024
ABSTRACT:
Background: Red blood cells go through a number of metabolic changes throughout the course of a 0-35 day storage period at 1 to 6oC. The sodium/potassium pump is immobilized, resulting in a drop in intracellular potassium with an increase in cytoplasmic sodium levels, a fall in glucose levels, acidosis as a result of low pH levels, and other alterations known as "storage lesions" during the storage phase. Thus, the purpose of this study was to assess the oxidative condition of red blood cells during storage, as well as certain related characteristics. Study Design and Methods: The whole blood was drawn and placed in a citrate-phosphate-dextrose adenine anticoagulant (CPDA). Blood samples were collected in the appropriate tube for each test at various storage times (zero, 7, 14, 21, and 35 days). All statistical analyses were carried out using SPSS ver. 26.0 (SPSS Inc., Chicago, IL, USA). Results: All the studied parameters showed significant differences at 35 days of storage when compared to zero time. The pH level decreases over the storage period. After 35 days, the average extracellular K+ level rose from 4.23 mmol/L at zero time to 29.86mmol/L. There was a slight increase in mean corpuscular volume (MCV) as a result of RBC storage. Discussion: This study demonstrated that, when care was made to assess pH and potassium ion concentrations to increase blood safety, red blood cells preserved in current storage media that retained their properties had acceptable transfusion quality.
Cite this article:
Raed Abd-Elrahman Alhyasat, Khaled Khleifat, Ali M. Khlaifat, Ahmad Za’al Alsarayreh, Yaseen Taha Al Qaisi, Maha N. Abu Hajleh, Moath Alqaraleh, Alia K. Ibrahim. Oxidative Stress and its connection to Jordanians' Red Blood Cell Storage. Research Journal of Pharmacy and Technology. 2024; 17(9):4304-0. doi: 10.52711/0974-360X.2024.00665
Cite(Electronic):
Raed Abd-Elrahman Alhyasat, Khaled Khleifat, Ali M. Khlaifat, Ahmad Za’al Alsarayreh, Yaseen Taha Al Qaisi, Maha N. Abu Hajleh, Moath Alqaraleh, Alia K. Ibrahim. Oxidative Stress and its connection to Jordanians' Red Blood Cell Storage. Research Journal of Pharmacy and Technology. 2024; 17(9):4304-0. doi: 10.52711/0974-360X.2024.00665 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-9-26
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