Author(s):
Arifah Sri Wahyuni, Riza Maulana, Maryati, Muhammad Da’i
Email(s):
muhammad.dai@ums.ac.id
DOI:
10.52711/0974-360X.2024.00616
Address:
Arifah Sri Wahyuni1, Riza Maulana2, Maryati3, Muhammad Da’i4*
1Department of Pharmacology, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Surakarta, Indonesia.
2Department of Pharmaceutics, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Surakarta, Indonesia.
3Department of PharmaceuticalBiology, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Surakarta, Indonesia.
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Surakarta, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 8,
Year - 2024
ABSTRACT:
Indonesia's abundant natural resources have spurred interest in exploring certain plants believed to possess aphrodisiac properties that can enhance male libido and reproductive health. This research endeavor is designed to delve into the extraction of compounds from various plant species, followed by a comprehensive analysis of their chemical constituents. Subsequently, aphrodisiac activity assessments are conducted on male rats, focusing on key parameters such as mating frequency, climbing behavior, and coital activities. Additionally, this investigation encompasses the determination of the percentage of relative testicular weight in relation to body weight and histopathological examination of testicular organs. The findings emanating from the extracts of Piper retrofractum (PRE), Solanum melongena fruit (EE), Ocimum basilicum leaves (OBLE), Watermelon albedo (WAE), and Zingiber officinale Var Rubrum rhizoma (ZRE) reveal the presence of alkaloids, flavonoids, and saponins. Notably, EE, administered at a dosage of 30mg, yields the most prominent aphrodisiac effects in the test subjects, as evidenced by heightened mating frequency, climbing activity, and coital behaviors. Furthermore, the relative testicular weight following treatment with 30mg of PRE closely resembles the impact observed with sildenafil at a dosage of 10 mg/kgbw.
Cite this article:
Arifah Sri Wahyuni, Riza Maulana, Maryati, Muhammad Da’i. Exploration of Indonesian Herbal Plants as Aphrodisiac agents and Testing their Activity in Animal Testing. Research Journal of Pharmacy and Technology.2024; 17(8):3969-5. doi: 10.52711/0974-360X.2024.00616
Cite(Electronic):
Arifah Sri Wahyuni, Riza Maulana, Maryati, Muhammad Da’i. Exploration of Indonesian Herbal Plants as Aphrodisiac agents and Testing their Activity in Animal Testing. Research Journal of Pharmacy and Technology.2024; 17(8):3969-5. doi: 10.52711/0974-360X.2024.00616 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-8-64
REFERENCES:
1. Situmorang EW, Kurniawati E, Soleha TU, Larasati T. Obesity and erectile dysfunction: Literature Review. PREPOTIF J Kesehat Masy. 2022; 6: 1258-1265. doi:10.31004/prepotif.v6i2.4336
2. Junairiah, Arofah J, Manuhara YSW, Nurhariyati T, Ni’matuzahroh. Effect of 2,4-d and bap on callus induction of piper retrofractum vahl. Res J Pharm Technol. 2021; 14(3): 1390-1394. doi:10.5958/0974-360X.2021.00248.1
3. Nagamani, K. Sesha Kumar, N. Sucharitha PVS. Male Sexual Dysfunction – An Over Looked Health Issue. A V Pub J Nurs Med Res. 2022; 1(1): 27-31. doi:10.52711/jnmr.2022.06
4. Singh R, Singh S, Jeyabalan G, Ali A, Semwal A. Sexual Dysfunction An Overview and Medicinal Plant used for treatment of Sexual dysfunction. Earthjournals Publ. 2012; 1(2): 9-24.
5. Luthfi MJ, Noor MM, Latip J. Medicinal Plants: A Prospect in Developing Male Fertility Enhancing Agent. Biol Med Nat Prod Chem. 2015; 4(2): 41. doi:10.14421/biomedich.2015.42.41-47
6. Kotta S, Ansari S, Ali J. Exploring scientifically proven herbal aphrodisiacs. Pharmacogn Rev. 2013; 7(13): 1-10. doi:10.4103/0973-7847.112832
7. Goldstein I, Goren A, Li VW, Tang WY, Hassan TA. Epidemiology Update of Erectile Dysfunction in Eight Countries with High Burden. Sex Med Rev. 2020; 8(1): 48-58. doi:10.1016/j.sxmr.2019.06.008
8. Wahyuni AS, Wahyuningtyas N, Arifiyanti. Aphrodisiac Activity Of Essential Oil From Syzygium aromaticum (L.) Merr. & Perry. Pharmacon. 2010; 11(2): 43-46.
9. Salleh MNH, Ahmad F. Phytopharmacological investigations of piper retrofractum vahl. – A review. Agric Conspec Sci. 2020; 85(3): 193-202.
10. Fajriyatun BN, Sutrisna E, Nirlawati DD. Investigating the Effects of 70% Etanol Extract from Oak Seeds (Solanum Melongena L.) on HDL Cholesterol Levels Male White Rat (Rattus Norvegicus) HDL Blood Serum Galur Wistar. Biomedika. 2014; 6(1): 13-16. doi:10.23917/biomedika.v6i1.282
11. Parisa N, Kamaluddin MT, Salni S. Aphrodisiac Effects of Areca Fruit in Erectile Dysfunction Rat Model. Biosci Med J Biomed Transl Res. 2019; 3(3): 16-23. doi:10.32539/bsm.v3i3.90
12. Dhanalakshmi S, Abinaya, Devi K, Lakshmi. In vitro anti-oxidant study of herbal extract mixture by nitric oxide and DPPH method. Res J Pharm Technol. 2017; 10(1): 277-280. doi:10.5958/0974-360X.2017.00057.9
13. Nurwaini S, Wikantyasning E. Formulations of Lozenges of Leaf Extract Ocimum sanctum L : The effect of sodium carboxymethyl cellulose as a binding substance on physical properties. J Penelit Sains Teknol. 2011; 12(1): 45-57. http://publikasiilmiah.ums.ac.id/handle/123456789/3434
14. Sharma R, Kumari N, Ashawat M., Verma CP. Standardization and Phytochemical Screening analysis for Herbal Extracts: Zingiber officinalis, Rosc., Curcuma longa Linn., Cinnamonum zeylanicum Nees., Piper longum, Linn., Boerhaavia diffussa Linn. Asian J Pharm Technol. 2020; 10(3): 127-133. doi:10.5958/2231-5713.2020.00022.7
15. Sharma M, Arya D, Bhagour K, Gupta RS. Natural aphrodisiac and fertility enhancement measures in males: A review. Curr Med Res Pract. 2017; 7(2): 51-58. doi:10.1016/j.cmrp.2017.02.007
16. Pantuzza Silva GF, Pereira E, Melgar B, et al. Eggplant fruit (Solanum melongena l.) and bio‐residues as a source of nutrients, bioactive compounds, and food colorants, using innovative food technologies. Appl Sci. 2021; 11(151): 1-24. doi:10.3390/app11010151
17. Zaini BSI, Pujiana IM, Djamaludin EO, Salim HM. The Effectiveness of Extract Basil Leaves on Motility and Morphology of Spermatozoa Mice Exposed with Cigarette Smoke. Biomol Heal Sci J. 2020; 3(2): 75. doi:10.20473/bhsj.v3i2.22169
18. Anandita DW, Nurlaila, Pramono S. Aphrodisiac Effects Of Red Ginger (Zingiber officinale Rosc. red clone) Essential Oil And Essential Oil Free Etanolic Extract In Male Rats. Maj Obat Tradisional,. 2012; 17(1): 8-14.
19. Wolfensohn S, Lloyd M. Handbook of Laboratory Animal Management and Welfare. Blackwell Scientific Publications Oxford and Edinburg; 2003. doi:10.1016/s0165-6147(00)89011-2
20. Tachikawa KS, Yoshihara Y, Kuroda KO. Sexual behavior and aggression in male mice: Involvement of the vomeronasal system. J Neurosci. 1984; 4(9): 2222-2229. doi:10.1523/jneurosci.04-09-02222.1984
21. Pal RS, Saraswat N, Pal Y, Wal P, Wal A, Rai AK. Alcoholic extract of poly herbal powder mixture for anti-obesity effect on wistar rats. Res J Pharm Technol. 2019; 12(4): 1857-1864. doi:10.5958/0974-360X.2019.00310.X
22. Islam MT. Phytochemical information and pharmacological activities of Okra (Abelmoschus esculentus): A literature-based review. Phyther Res. 2019; 33(1): 72-80. doi:10.1002/ptr.6212
23. Supu RD, Diantini A, Levita J, et al. Red Ginger ( Zingiber officinale var. rubrum): Its Chemical Constituents, Pharmacological Activities And Safety. Fitofarma. 2018; 8(1): 25-31.
24. Assefa AD, Hur OS, Ro NY, et al. Fruit morphology, citrulline, and arginine levels in diverse watermelon (Citrullus lanatus) germplasm collections article. Plants. 2020; 9(9): 1-16. doi:10.3390/plants9091054
25. Srivatsav A, Balasubramanian A, Pathak UI, et al. Efficacy and Safety of Common Ingredients in Aphrodisiacs Used for Erectile Dysfunction: A Review. Sex Med Rev. 2020; 8(3): 431-442. doi:10.1016/j.sxmr.2020.01.001
26. Khair-ul-Bariyah S, Ahmed D, Ikram M. Ocimum Basilicum: A Review on Phytochemical and Pharmacological Studies. Pakistan J Chem. 2012; 2(2): 78-85. doi:10.15228/2012.v02.i02.p05
27. Igwebuike UM, Ochiogu IS, Ihedinihu BC, Ikokide JE, Idika IK. The effects of oral administration of monosodium glutamate (msg) on the testicular morphology and cauda epididymal sperm reserves of young and adult male rats. Vet Arh. 2011; 81(4): 525-534.
28. Bairagi G., Kabra A., Mandade R. Sexual dysfunction in men with diabetes. Res J Pharm Tech. 2011; 4(5): 677-684.
29. Samala A, Pawar S, Manala S, Chada S, Nageshwar M. RP-HPLC method development and validation of tadalafil in tablet dosage form. J Chem Pharm Res. 2013; 5(4): 315-318. doi:10.52711/0974-4150.2021.00065
30. Arcaniolo D, Imbimbo C, Grillo M, Fusco F. Vardenafil for the treatment of erectile dysfunction. Clin Pract. 2012; 9(1): 39-48. doi:10.2217/CPR.11.131
31. Dadras J, Herman N, Rahimi S. The study of herbal medicines and their therapeutic effects for farm animals. Asian J Pharm Technol. 2020; 10(3): 137. doi:10.5958/2231-5713.2020.00024.0
32. Kusumawati I, Mahatmaputra S, Hadi R, et al. Aphrodisiac Activity of Ethanolic Extracts from the Fruits of Three Pepper Plants from Piperaceae Family. J Farm Dan Ilmu Kefarmasian Indones. 2021; 8(2): 194. doi:10.20473/jfiki.v8i22021.194-199
33. D’Cruz SC, Mathur PP. Effect of piperine on the epididymis of adult male rats. Asian J Androl. 2005; 7(4): 363-368. doi:10.1111/j.1745-7262.2005.00059.x
34. Munglue P, Kupittayanant S, Kupittayanant P. Effect of watermelon (Citrullus lanatus) flesh extract on sexual behavior of male rats. Chiang Mai Univ J Nat Sci. 2014; 13(1): 519-527. doi:10.12982/cmujns.2014.0054
35. Cormio L, De Siati M, Lorusso F, et al. Oral L-citrulline supplementation improves erection hardness in men with mild erectile dysfunction. Urology. 2011; 77(1): 119-122. doi:10.1016/j.urology.2010.08.028
36. Jain MS, Koradia SK. Phosphodiesterase-5 (PDE5) Inhibitors in the Treatment of Erectile Dysfunction: A Review. Asian J Pharm Res. 2023; 13(1). doi:10.52711/2231-5691.2023.00012
37. Lasker GF, Pankey EA, Kadowitz PJ. Modulation of soluble guanylate cyclase for the treatment of erectile dysfunction. Physiology. 2013; 28(4): 262-269. doi:10.1152/physiol.00001.2013
38. Kagathara V, Vyawahare N, Pujari R, Patil M, Ansari I, Bhandare A. Effect of Chlorophytum borivilianum on Fluoxetine Induced Sexual Dysfunction in Female Rats. Res J Pharmacol Pharmacodyn. 2009; 1(3): 130-133.
39. Carro-Juárez M, Alcazar C, Ballesteros-Polvo E, Villalobos-Peñalosa P. Increase of ejaculatory capacity by systemic administration of the oquichpatli (Senecio cardiophyllus) aqueous crude extract in male rats. J Ethnopharmacol. 2009; 126(3): 506-511. doi:10.1016/j.jep.2009.09.006
40. Singh S, Nair V, Gupta YK. Evaluation of the aphrodisiac activity of Tribulus terrestris Linn. in sexually sluggish male albino rats. J Pharmacol Pharmacother. 2012; 3(1): 43-47. doi:10.4103/0976-500X.92512