ABSTRACT:
Triple Negative Breast Cancer (TNBC) is a highly aggressive type of breast cancer which surpassed the death rate of lung cancer. Hence, discovery of new effective drugs is of immediate clinical need. The objective of the study was to identify and isolate terpenoids from the marine red alga Hypnea valentiae and study its potential cytotoxic and anti-invasive properties in TNBC cell line. The alga was extracted using ethyl acetate. The ethyl acetate extract (HVEA) was separated by column chromatography. The isolated fractions F1 and F2 were characterized by GC-MS. Followed by this F1 and F2 were analyzed for their cytotoxic activity under in vitro conditions in MDA MB 231 cell line using cell proliferative assay and their antimetastatic activity was determined by scratch assay. The outcomes of GC-MS analysis showed that F1 was a terpenoid n-hexadecenoic acid and F2 was a mixture of tetradecanoic acid, n-hexadecenoic acid, cyclohexanol 1-ethnyl carbamate and caryophyllene oxide. The results of in vitro studies showed that HVEA, F1 and F2 exhibited cytotoxicity in MDA-MB 231 cells with IC50 of 10µg/ml, 15µg/ml and 25µg/ml respectively. Further the findings of scratch assay (24hrs) showed that HVEA, F1 and F2 prevented the invasion of cells into the wound area. Overall, the findings of the current investigation suggest that HVEA and isolated terpenoids have potential activity against the survival and invasion of TNBC cells. Hence these compounds may find prominent application in cancer therapy that can be obtained from marine algae.
Cite this article:
S. Jazeera Mariyam, S.M. Fazeela Mahaboob Begum. Cytotoxic Marine Terpenes from Hypnea valentiae inhibit cell viability and migration of TNBC cells under In vitro conditions. Research Journal of Pharmacy and Technology. 2024; 17(8):3575-1. doi: 10.52711/0974-360X.2024.00558
Cite(Electronic):
S. Jazeera Mariyam, S.M. Fazeela Mahaboob Begum. Cytotoxic Marine Terpenes from Hypnea valentiae inhibit cell viability and migration of TNBC cells under In vitro conditions. Research Journal of Pharmacy and Technology. 2024; 17(8):3575-1. doi: 10.52711/0974-360X.2024.00558 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-8-6
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