Author(s):
Tamilselvan Natarajan, Prasanth K. G., Mahendran S., Jincy V. Varghese, Divya Gupta P.K., Sneha V.R., Sufairath, Reshma E.S., Chinnu. P.C.
Email(s):
tselvan24@gmail.com
DOI:
10.52711/0974-360X.2024.00591 
Address:
Tamilselvan Natarajan1*, Prasanth K. G.2, Mahendran S.3, Jincy V. Varghese4, Divya Gupta P.K.5, Sneha V.R.6, Sufairath7, Reshma E.S.8, Chinnu. P.C.9
1Department of Pharmaceutics, Nehru College of Pharmacy, Pampady, Kerala, India.
2Department of Pharmacology, Nehru College of Pharmacy, Pampady, Kerala, India.
3-9Department of Pharmaceutics, Nehru College of Pharmacy, Pampady, Kerala, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 8,
Year - 2024
ABSTRACT:
Recently, researchers have looked at the use of nanotechnology as a drug-delivery system for topical and transdermal applications. The transport of medications and active ingredients to the skin via formulations, including nanoparticles, is a subject of substantial contemporary interest. The present work is proposed to prepare the atorvastatin nanogels to promote wound healing in a diabetic animalmodel.Atorvastatin nanogels were prepared by precipitation polymerization technique using hydroxy propyl methacrylate as polymer. The drug and polymer were selected in ratios of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7 and 1:8. Additionally, the produced nanogel was evaluated in vivo and examined for its particle size, trapping effectiveness, and drug release in vitro. The particle size of the prepared various formulations (F1-F8) showed a size range of 68 to 80nm, and entrapment efficiency was seen to be in the range of 58.36-86.75%. The cumulative percentage of drug release was reported to be 61.96 to 73.76 percent over a period of 12hours during the in vitro drug release investigation, which was conducted using phosphate buffer at pH 7.4.The drug release followeda non-fiction mechanism of release kinetics. On the other hand, in vivo comparative study showed a complete wound healing effect for nanogels on the 11th day, whereas for conventional gel on the 15th day.This study indicates that nanogels formulation heals the diabetic wound completely at a faster rate, and also the drug atorvastatin also can be used for diabetic wound healing.
Cite this article:
Tamilselvan Natarajan, Prasanth K. G., Mahendran S., Jincy V. Varghese, Divya Gupta P.K., Sneha V.R., Sufairath, Reshma E.S., Chinnu. P.C.. Development and In vivo Evaluation of Nanogel Drug Delivery System for Promoting Wound Healing in Diabetic Induced Rats. Research Journal of Pharmacy and Technology. 2024; 17(8):3807-3. doi: 10.52711/0974-360X.2024.00591
Cite(Electronic):
Tamilselvan Natarajan, Prasanth K. G., Mahendran S., Jincy V. Varghese, Divya Gupta P.K., Sneha V.R., Sufairath, Reshma E.S., Chinnu. P.C.. Development and In vivo Evaluation of Nanogel Drug Delivery System for Promoting Wound Healing in Diabetic Induced Rats. Research Journal of Pharmacy and Technology. 2024; 17(8):3807-3. doi: 10.52711/0974-360X.2024.00591 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-8-39
REFERENCES:
1. Amit Naina, Yu-Ting Tsenga, Akash Guptab, Yu-Feng Lina, Arumugam Sangilia, Yu-Fe et al. Anti-microbial/ oxidative/ inflammatory, nanogels accelerate chronic wound healing. Smart Materials in Medicine. 2022; 3: 148-158. DOI: 10.1016/j.smaim.2021.12.006
2. Yang Yang, Bo Zhang, Yufan Yanga, Bibo Peng, Rui Ye. Diabetes Research and Clinical Practice. 2022; 184: 109-121 DOI: 10.1016/j.diabres.2010.05.015
3. Nicole Nowak, Daniela M. Menichella, Richard Miller, Amy S. Palle. Cutaneous innervation in impaired diabetic wound healing. Translational Research. 2021; 236: 87-108. doi.org/10.1016/j.trsl.2021.05.003
4. Robin Augustine, Anwarul Hasan, Yogesh B. Dalvi, Syed Raza Ur Rehman, Ruby Varghese, Raghunath Narayanan Unni.et al. Growth factor loaded in situ photocrosslinkablepoly (3-hydroxybutyrate-co-3-hydroxyvalerate)/ gelatin methacryloyl hybrid patch for diabetic wound healing, Materials Science and Engineering: C. 2021; 118: 111519. DOI: 10.1016/j.msec.2020.111519
5. Satish Patel, Shikha Srivastava, Manju Rawat Singh, Deependra Singh. Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing, Biomedicine and Pharmacotherapy. 2019; 112: 108615. DOI: 10.1016/j.biopha.2019.108615
6. Yuanping Hao, Wenwen Zhao, Hao Zhang, Weiping Zheng, Qihui Zhou. Carboxymethyl chitosan-based hydrogels containing fibroblast growth factors for triggering diabetic wound healing. Carbohydrate Polymers. 2022; 287; 119336. DOI: 10.1016/j.carbpol.2022.119336
7. Maryam Shafique, Muhammad Sohail, Muhammad Usman Minhas, Touba Khaliq, Mubeen Kousar, Shahzeb Khan, et al. Bio-functional hydrogel membranes loaded with chitosan nanoparticles for accelerated wound healing. International Journal of Biological Macromolecules. 2021; 170; 207-221. https://doi.org/10.1016/j.ijbiomac.2020.12.157
8. Heni Wang, Zejun Xu, Meng Zhao, Guiting Liu, Jun Wu. Advances of hydrogel dressings in diabetic wounds. Biomaterials Science. 2021; 5: 1530-1546. https://doi.org/10.1039/D0BM01747G
9. Wei Wang, Kong-jun Lu, Chao-heng Yu, Qiao-ling Huang, Yong-Zhong Du. Nano-drug delivery systems in wound treatment and skin regeneration. Journal of Nanobiotechnology. 2019; 17: 1-15. https://doi.org/10.1186/s12951-019-0514-y
10. Yaqing Huang, Themis R. Kyriakides. The role of extracellular matrix in the pathophysiology of diabetic wounds. Matrix Biology Plus. 2020; 6-7: 100037. doi: 10.1016/j.mbplus.2020.100037
11. Van Vlierberghe, S, Dubruel, P, Schacht, E. Biopolymer-Based Hydrogels As Scaffolds for Tissue Engineering Applications: A Review. Biomacromolecules. 2011; 12(5): 1387-1408. https://doi.org/10.1021/bm200083n
12. Duraisamy Y, Slevin, M, Smith N. Effect of glycation on basic fibroblast growth factor induced angiogenesis and activation of associated signal transduction pathways in vascular endothelial cells: possible relevance to wound healing in diabetes. Angiogenesis. 2001; 4: 277–88. DOI: 10.1023/a:1016068917266
13. Steed D.L, Attinger C, Colaizzi T. Guidelines for the treatment of diabetic ulcers. Wound Repair Regen. 2006; 14: 680–92. doi: 10.1111/j.1524-475X.2006.00176.x.
14. Hitesh Patel A, Jayvadan Patel K. Nanogel as a controlled drug delivery system. Int. J. Pharm. Sci. Rev. Res. 2010; 4 (2): 37-41.
15. Olivera Stojadinovic, Elizabeth Lebrun, Irena Pastar, Robert Kirsner, Stephen C. Davis and marjanatomic-Canic, Statins as potential therapeutic agents for healing disorders: expert commentary. Wound Healing and Regenerative Medicine. Research Program. 2010. DOI: 10.1586/edm.10.60
16. Toker S, Gulcan E, Cayc M.K, Olgun E.G, Erbilen E, Ozay Y. Topical atorvastatin in the treatment of diabetic wounds. Am. J. Med. Sci. 2009; 338(3): 201–204. DOI: 10.1097/MAJ.0b013e3181aaf209
17. Nor Abu Samah, Nicholas Williams, Charles, M. Heard, Nanogel particulates located within diffusion cell receptor phases following topical application demonstrates uptake into and migration across skin. Int. J. Pharm. 2010; 401: 72–78. DOI: 10.1016/j.ijpharm.2010.08.011
18. Mulla Jameel Ahmed S, Shetty Nitinkumar S, Panchamukhi Shridhar I, Khazi Imtiyaz Ahmed M. Formulation, characterization and in vitro evaluation of novel thienopyrimidines and triazolothienopyrimidines loaded solid lipid nanoparticles. I.J.R.A.P. 2010; 1(1): 192-200.
19. Belma Isik. Swelling behavior of acrylamide-2-hydroxyethyl methacrylate hydrogels. Turk. J. Chem. 2000; 24: 147- 156. /journals.tubitak.gov.tr/chem/vol24/iss2/5
20. Najmuddin M, Mohsin A.A, Tousif Khan Patel, V, Shelar S. Formulation and evaluation of solid dispersion incorporated gel of ketoconazole. R.J.P.B.C.S. 2010; 1(2): 406- 412.
21. Fathy Abd-Allah I, Hamdy Dawaba M, Ahmed M. S. Preparation, characterization, and stability studies of piroxicamloaded microemulsions in topical formulations. Drug Dis. and Therap. 2010; 4(4): 267-275.
22. Al Bayaty F, Abdulla M, Abu Hassan M. I, Masud M. Wound healing potential by hyaluronate gel in streptozotocin-induced diabetic rats. Sci. Res. Essays. 2010; 5(18): 2756-2760.
23. Manish Pal Singh, Chandra Shekhar Sharma. Wound healing activity of Terminalia chebula in experimentally induced diabetic rats. Int. J. Pharm Tech Res. 2009; 1(4) : 1267-1270.