Author(s):
Indri Ngesti Rahayu, Asami Rietta Kumala, Dody Taruna, Stefanus Djoni Husodo, Eric Mayo Dagradi, Judya Sukmana
Email(s):
drindringestirahayu@gmail.com
DOI:
10.52711/0974-360X.2024.00573 
Address:
Indri Ngesti Rahayu1*, Asami Rietta Kumala1, Dody Taruna1, Stefanus Djoni Husodo1, Eric Mayo Dagradi1, Judya Sukmana2
1Lecturer, Department of Physiology, Faculty of Medicine, Hang Tuah University, Jl. Gadung 1 West Wing of Ramelan Navy Hospital Surabaya, Surabaya, East Java, Indonesia.
2Lecturer, Department of Pathology, Faculty of Medicine, Hang Tuah University, Jl. Gadung 1 West Wing of Ramelan Navy Hospital Surabaya, Surabaya, East Java, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 8,
Year - 2024
ABSTRACT:
Background: Background: Type 2 Diabetes Mellitus (T2DM) is characterized by disrupted glucose metabolism, leading to hyperglycemia and insulin resistance, often associated with Secondary Hypertension (SH). Over 80% of SH patients exhibit glucose intolerance, with nearly 30% developing T2DM. There is a strong interaction between T2DM and NAFLD, representing a complex two-way relationship that influences the prognosis of the two diseases. Catfish oil extract boasts unsaturated fatty acids, including DHA, EPA, omega-3, vitamins A, B6, B12, D, and amino acids. Objective: This study aims to assess the impact of Pangasius sp. (catfish) oil extract administration on serum AST and ALT levels, as well as liver tissue MDA and CAT levels in alloxan-induced Wistar rats (Rattus norvegicus). Materials and Methods: Employing a pure experimental approach with a post-test-only control group design, three groups of 9 white male rats (Rattus norvegicus), along with one extra male rat, were included.K1 served as the negative control group (Wistar rats not subjected to any treatment). K2 acted as the positive control group (Wistar rats subjected to alloxan-induced diabetes at 150mg/kg BW). K3 represented the treatment group (diabetic model rats treated with catfish oil extract at 73mg/kg BW). Serum AST and ALT levels, as well as MDA and CAT levels in liver tissue, were measured at the study's conclusion. Results: The highest mean MDA levels in white rat liver tissue were K2 (3034.00 + 525.25 nmol/g), and the lowest was K3(2909.33+351.01nmol/g); the mean CAT liver tissue levels in rats the highest was K1 (1063.42+133.36U/mg), and the lowest was K3(894.78+132.93U/mg), the highest mean serum ALT levels of white rats was K2(230.34+63,58 U/L), and the lowest was K1(151.54+23.12 U/L) and the highest mean serum AST level in rats was K2(448.79+618.90U/L), and the lowest was K1(61.01+14.70U/L). Conclusion: Giving catfish oil supplementation can repair the damage in liver tissue, as evidenced by reductions in MDA levels and serum ALT and AST levels in diabetic model rats within the treatment group. Alloxan induction did not affect liver tissue CAT levels, as evidenced by a consistent decrease in liver tissue CAT levels in both the positive control and treatment groups.
Cite this article:
Indri Ngesti Rahayu, Asami Rietta Kumala, Dody Taruna, Stefanus Djoni Husodo, Eric Mayo Dagradi, Judya Sukmana. Research Journal of Pharmacy and Technology. 2024; 17(8):3676-4. doi: 10.52711/0974-360X.2024.00573
Cite(Electronic):
Indri Ngesti Rahayu, Asami Rietta Kumala, Dody Taruna, Stefanus Djoni Husodo, Eric Mayo Dagradi, Judya Sukmana. Research Journal of Pharmacy and Technology. 2024; 17(8):3676-4. doi: 10.52711/0974-360X.2024.00573 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-8-21
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