Author(s): Rupesh S. Kulkarni, Mahesh S. Palled

Email(s): rupesh.k88@gmail.com , pjpalled@gmail.com

DOI: 10.52711/0974-360X.2024.00453   

Address: Rupesh S. Kulkarni1,2, Mahesh S. Palled1*
1Department of Pharmaceutical Chemistry, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, India. 2KLES’s College of Pharmacy, Nipani-591237, Karnataka, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Aim: The present study deals with development and validation of accurate, sensitive and cost-effective RP-HPLC method for Loxoprofen sodium. Method: PRIMESIL C18 column (id 4.6 x 250 mm length) was used as stationary phase and a mobile phase used was mixture of methanol and 0.05% OPA buffer in ratios of 75:25 v/v. The 20µl samples were injected at a flow rate of 1 ml/min. The detector used is G-13148 (DAD) detector, with a wavelength observed of 225nm at ambient column temperature. Further, the method developed was validated as per ICH guidelines. Results: The method's linearity was studied at concentrations between 5 and 25µg/ml, wherein the results were found to be linear with correlation coefficient (r2) values of 0.999. The limits of detection and quantitation for developed method were observed to be 0.09175 and 0.278032 µg/ml respectively. The intraday and interday precision results were analysed and found to be within 2%. The percentage assay of the marketed product was found to be 101.85±0.090%. The percentage recovery of the drug was ranged between 97.7 1% to 101.09%. during accuracy study.


Cite this article:
Rupesh S. Kulkarni, Mahesh S. Palled. A Rapid, Sensitive and Cost-Effective RP-HPLC Method for Determination of Loxoprofen Sodium. Research Journal of Pharmacy and Technology. 2024; 17(6):2889-4. doi: 10.52711/0974-360X.2024.00453

Cite(Electronic):
Rupesh S. Kulkarni, Mahesh S. Palled. A Rapid, Sensitive and Cost-Effective RP-HPLC Method for Determination of Loxoprofen Sodium. Research Journal of Pharmacy and Technology. 2024; 17(6):2889-4. doi: 10.52711/0974-360X.2024.00453   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-72


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