Author(s): Hasnah Natsir, Ahyar Ahmad, Nasrum Massi, Paulina Taba, Anita, Wahyudin Rauf

Email(s): hasnahnatsir@unhas.ac.id , hasnahnatsir@gmail.com

DOI: 10.52711/0974-360X.2024.00448   

Address: Hasnah Natsir1*, Ahyar Ahmad1, Nasrum Massi 2, Paulina Taba1, Anita3,4, Wahyudin Rauf3
1Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar 90245, Indonesia.
2Microbiology Department, Medicine Faculty, Hasanuddin University, Makassar 90245, Indonesia.
3 Doctoral Program of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar 90245, Indonesia.
4Medical Laboratory Technology, Polytechnic of Muhammadiyah Makassar, Makassar 90132, Indonesia.
5Magister Program of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University, Makassar 90245, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Protease is an enzyme that hydrolyses peptide bonds in amino acid residues of proteins and is essential for many metabolic processes. However, in the development of biotechnology, proteolytic enzymes play an important role in various industrial processes, including in the detergent, leather, textile, food, and pharmaceutical industries. Marine sediment ecosystems provide a diversity of microorganisms with the ability to produce the extracellular proteolytic enzyme. Compared to other varieties, marine bacteria are a more profitable and easier-to-produce source of protease because they are unique, specific, and resistant to salinity, temperature, light, and extreme environments. This research aims to isolate and characterize protease-marine sediment bacteria as an antimicrobial application. Samples were collected from marine sediments in Losari Coastal, Makassar City, South Sulawesi, Indonesia, and isolated bacteria were subjected to primary and secondary screening to select potential protease producers. The isolated bacteria were identified using macroscopy, microscopy, and gene sequencing (16S rRNA). The extracellular protease enzyme will be continued for antimicrobial applications. Based on the results, proteolytic MBS-L3 isolate obtained from marine sediments are capable of hydrolysing skim milk and casein substrates at 40oC. Next, the MBS-L3 isolate was identified as a round colony with a scalloped margin, a smooth, convex shape, and bacilli or rod-shaped gram-negative bacteria. Molecular characterization revealed that the MBS-L3 isolate has 98.893% identity to Vibrio alginolyticus strain NRBC 15630. The extracellular protease of the MBS-L3 isolate was exhibited against Staphylococcus aureus ATCC 25922 and against Escherichia coli ATCC 25923. According to these findings, proteolytic MBS-L3 isolates from marine sediments provide potential opportunities for new drugs for commercial use in the fields of medicine, pharmaceuticals, and biotechnology.


Cite this article:
Hasnah Natsir, Ahyar Ahmad, Nasrum Massi, Paulina Taba, Anita, Wahyudin Rauf. Isolation, Production of Protease, and Antimicrobial Activities from Marine Sediment Gamma - Proteobacteria of MBS-L3 Isolate. Research Journal of Pharmacy and Technology. 2024; 17(6):2855-2. doi: 10.52711/0974-360X.2024.00448

Cite(Electronic):
Hasnah Natsir, Ahyar Ahmad, Nasrum Massi, Paulina Taba, Anita, Wahyudin Rauf. Isolation, Production of Protease, and Antimicrobial Activities from Marine Sediment Gamma - Proteobacteria of MBS-L3 Isolate. Research Journal of Pharmacy and Technology. 2024; 17(6):2855-2. doi: 10.52711/0974-360X.2024.00448   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-67


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