Author(s): Pai D, Adiga S, Suresh G, Adiga U, Kumari S, Chaitra D, Desy TM

Email(s): adigaiscool@yahoo.com

DOI: 10.52711/0974-360X.2024.00444   

Address: Pai D1, Adiga S2*, Suresh G3, Adiga U4, Kumari S5, Chaitra D6, Desy TM7
1Tutor, Dept. of Anatomy, KS Hegde Medical Academy, Mangalore, Karnataka, India.
2Professor, Dept. of Pharmacology, Apollo Institute of Medical Sciences and Research Chittoor, India.
3Professor, Dept. of General Medicine, KS Hegde Medical Academy, Mangalore, Karnataka, India.
4Professor, Dept. of Biochemistry, Apollo Institute of Medical Sciences and Research Chittoor, India.
5Professor, Dept. of Biochemistry, KS Hegde Medical Academy, Mangalore, Karnataka, India.
6Tutor, Dept. of Anatomy, KS Hegde Medical Academy, Mangalore, Karnataka, India.
7Research Scholar, Central Research Lab, KS Hegde Medical Academy, Mangalore, Karnataka, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
The aim of the study was to compare sirtuin 1 serum levels in non-insulin dependent diabetics and diabetic nephropathy patients, and evaluate the pattern of polymorphism of SIRT 1 gene in these patients, and find the relation between polymorphism of SIRT1 gene and sirtuin1 serum levels in diabetic nephropathy patients and those with various stages of diabetic nephropathy. Methodology: 108 type-2 diabetic patients without complications as controls and 108 diabetic nephropathy patients as the case group were included in the study. SIRT 1 expression was measured by ELISA, and SIRT1 gene polymorphism was analyzed using the PCR-RFLP method. Results: The mean serum sirtuin 1 level were significantly lower in diabetic nephropathy patients compared to controls (p=0.000). The distribution of genotypes did not conform to Hardy-Weinberg equilibrium. The frequency of the wild-type genotype (AA) was higher in the case group, while the mutant allele (AG+GG) was more prevalent in controls. The distribution of genotypes did not conform to Hardy-Weinberg equilibrium (chi-square =7.203, p=0.027). There was no significant association observed between SIRT1 gene polymorphism and serum sirtuin 1 level in diabetic nephropathy patients(p=0.001). Additionally, no significant difference was found in serum sirtuin 1 level between different stages of diabetic nephropathy based on albuminuria testing and estimated glomerular filtration rate (eGFR)(p=0.33). Conclusion: Patients with diabetic nephropathy exhibited significantly lower serum sirtuin 1 level compared to controls, suggesting a potential role of sirtuin 1 in the pathogenesis of DN. We also conclude that serum SIRT 1 expression may be used as a diagnostic marker. The results indicate a need for further research to better understand the role of SIRT1 in diabetic nephropathy and its potential as a biomarker or therapeutic target for this condition.


Cite this article:
Pai D, Adiga S, Suresh G, Adiga U, Kumari S, Chaitra D, Desy TM. Serum SIRT1 Levels and Genetic Variants in Diabetic Nephropathy: Insights from a Cross-sectional study. Research Journal of Pharmacy and Technology. 2024; 17(6):2829-4. doi: 10.52711/0974-360X.2024.00444

Cite(Electronic):
Pai D, Adiga S, Suresh G, Adiga U, Kumari S, Chaitra D, Desy TM. Serum SIRT1 Levels and Genetic Variants in Diabetic Nephropathy: Insights from a Cross-sectional study. Research Journal of Pharmacy and Technology. 2024; 17(6):2829-4. doi: 10.52711/0974-360X.2024.00444   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-63


REFERENCES:
1.    Rezkita F. Wibawa KG. Nugraha AP. Curcumin loaded Chitosan Nanoparticle for Accelerating the Post Extraction Wound Healing in Diabetes Mellitus Patient: A Review. Research J. Pharm. and Tech. 2020; 13(2): 1039-1042. doi.org/10.5958/0974-360X.2020.00191.2
2.    Tandi Joni. Maila NFR. Herlambang W. Nurzafika. Viani Anggi. Handayani TW. Test effects of Ethanol Jatropha gossypifolia L extract against Diabetic Nephropathy. Research Journal of Pharmacy and Technology. 2022; 15(9): 3841-6. doi.org/10.52711/0974-360X.2022.00644
3.    Munish Kakkar. Shreeja Singh. Tapan Behl. Sukhbir Singh. Neelam Sharma. Hema. Monika Sachdeva. Update on the role of Angiogenesis in Diabetes associated Nephropathy. Research Journal of Pharmacy and Technology. 2021; 14(7): 3947-4. doi.org/10.52711/0974-360X.2021.00685  
4.    Abbas Sabbar Dakhil. Association of the ATP-binding Cassette Transporter A1 Gene Polymorphism with Lipid Profile and Type 2 Diabetes Mellitus. Research J. Pharm. and Tech 2019; 12(10): 4657-4662. doi.org/10.5958/0974-360X.2020.00201.2
5.    Astha Jaiswal. Bhupesh Chandra Semwal. Sonia Singh. A Compressive Review on Novel Molecular Target of Diabetic Nephropathy. Research Journal of Pharmacy and Technology. 2022; 15(3): 1398-4. doi.org/10.52711/0974-360X.2022.00233
6.    Wolf G. Sharma K. Pathogenesis, Clinbical manifestations, and Natural History of Diabetic Nephropathy. Comprehensive Clinical Nephrology. Elsevier Saunders.2000. doi.org/10.1159/000324939
7.    Tamilisetti Vidya Sagar. Yatish Byndoor. Anupam Das. Prescribing pattern of Antihypertensive, Anti-Diabetic, Hypolipidaemic and Anti-Obesity Drugs in Diabetic Nephropathy. Research Journal of Pharmacy and Technology. 2022; 15(5): 2240-3. doi.org/ 10.52711/0974-360X.2022.00372
8.    Gupta S. Dominguez M. Golestaneh L. Diabetic Kidney Disease: An Update. Medical Clinics of North America. 2023; 107 (4): 689-705. doi.org/10.1016/j.mcna.2023.03.004
9.    Nur Samsu. Setyawati Soeharto. Muhaimin Rifai. Achmad Rudijanto. The Superiority of Rosmarinic Acid over Vitamin E in Preventing Development and Inhibiting Progression of Diabetic Nephropathy in Rats. Asian J. Pharm. Res. 2020; 10(3): 131-139. doi/org/10.5958/2231-5691.2020.00025.8  
10.    Jaiswal A. Semwal BC. Singh S. A Compressive Review on Novel Molecular Target of Diabetic Nephropathy. Research Journal of Pharmacy and Technology. 2022; 15(3): 1398-4. doi.org/10.52711/0974-360X.2022.00233
11.    Afkarian  M. Sachs  MC. Kestenbaum  B. Hirsch  IB. Tuttle  KR.  Himmelfarb J. et al. Kidney disease and increased mortality risk in Type 2 diabetes. J Am Soc Nephrol. 2013; 24(2): 302-308. doi.org/10.1681/ASN.2012070718.
12.    Vadivelan R. Umasankar P. Dipanjan Mandal. Shanish A. Dhanabal SP. Elango K. Oxidative Stress Induced Diabetic Nephropathy. Research J. Pharmacology and Pharmacodynamics. 2010; 2(5): 321-323. doi.org/ 10.5958 2321-5836
13.    Maysoon Mohammed Hassan. The significance of interleukin 4 (IL-4) (590-C/T) gene polymorphism in Iraqi patients with type 2 diabetes mellitus: A case-control study. Research J. Pharm. and Tech. 2019; 12(11): 5133-5137. doi.org/0.5958/0974-360X.2019.00889.8
14.    Rao V. Rao V. Tan SH. Candasamy M. Bhattamisra SK. Diabetic nephropathy: An update on pathogenesis and drug development. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2019; 13(1): 754-762. doi.org/10.1016/j.dsx.2018.11.054.
15.    Kurylowicz A. In search of new therapeutic targets in obesity treatment: Sirtuins. Int. J. Mol. Sci. 2016; 17(4): 572.doi.org/ 10.3390/ijms17040572
16.    Kong L. Wu H. Zhou W. Luo M. Tan Y. Miao L. Cai L. Sirtuin 1: A Target for Kidney Diseases. Mol Med. 2015; Jan 12; 21(1): 87-97. doi.org/10.2119/molmed.2014.00211
17.    Kume S. Thomas MC. Koya D. Nutrient sensing, autophagy, and diabetic nephropathy. Diabetes. 2012; 61: 23–9. doi.org/10.2337/db11-0555
18.    Yoshizaki T. Schenk S. Imamura T. Babendure JL. Sonoda N. Ju Bae E. et al. SIRT1 inhibits inflammatory pathways in macrophages and modulates insulin sensitivity. Am J Physiol Endocrinol Metab. 2010; 298(3): 419-28. doi.org/ 10.1152/ajpendo.00417.2009.
19.    Wang W. Sun W. Cheng Y. Xu Z. Cai L. Role of sirtuin-1 in diabetic nephropathy. J Mol Med (Berl) 2019; 97(3): 291-309. doi.org/10.1007/s00109-019-01743-7.
20.    Kitada M. Kume S. Takeda-Watanabe A. Kanasaki K. Koya D. Sirtuins and renal diseases: Relationship with aging and diabetic nephropathy. Clin Sci (Lond) 2013; 124 (Pt3): 153-64. doi.org/10.1042/CS20120190
21.    Zhang A. Wang H. Qin X. Pang S. Yan B. Genetic analysis of SIRT1 gene promoter in sporadic Parkinson’s disease. Biochemical and Biophysical Research Communications. 2012; 422 (4): 693-696. doi.org/10.1016/j.bbrc.2012.05.059
22.    Letonja J. Zavrsnik M. Makuc J. Seruga M. Peterlin A. Cilensek I. et al., Sirtuin 1 rs7069102 polymorphism is associated with diabetic nephropathy in patients with type-2 diabetes mellitus. Bosn J Basic Med Sci. 2021; 21(5): 642-646. doi.org/ 10.17305/bjbms.2020.5368
23.    Yue XG. Yang ZG. Zhang Y. Qin GJ. Liu F. Correlations between SIRT1 gene polymorphisms and diabetic kidney disease. R Soc Open Sci. 2018; Jun 13; 5(6): 171871. doi.org/10.1098/rsos.171871
24.    Sun Y. Wang J. Meng Y. Correlation Between Polymorphisms of the SIRT1 Gene microRNA Target Sites and Diabetic Nephropathy. Genet Test Mol Biomarkers. 2021; 25(6): 387-398. doi.org/10.1089/gtmb.2020.0261
25.    Zhao Y. Wei J. Hou X. SIRT1 rs10823108 and FOXO1 rs17446614 responsible for genetic susceptibility to diabetic nephropathy. Sci Rep. 2017; 7(1): 10285. doi.org/10.1038/s41598-017-10612-7
26.    Tang K. Sun M. Shen J. Zhou B. Transcriptional Coactivator p300 and Silent Information Regulator 1 (SIRT1) Gene Polymorphism Associated with Diabetic Kidney Disease in a Chinese Cohort. Exp Clin Endocrinol Diabetes. 2017; 125(8): 530-537. doi.org/10.1055/s-0043-103966.
27.    Gok O. Karaali Z. Ergen A. Ekmekci SS. Abaci N. Serum sirtuin 1 protein as a potential biomarker for type 2 diabetes: Increased expression of sirtuin 1 and the correlation with microRNAs. J Res Med Sci. 2019; Jun 25; 24: 56. doi.org/ 10.4103/jrms.JRMS_921_18
28.    Shao Y. Ren H. Lv C. Ma X. Wu C. Wang Q.  Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. Endocrine. 2017; 55(1): 130–138. doi.org/10.1007/s12020-016-1069-4


Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available