Author(s): Priyanka Pallapolu, Lahari Kuna, Alokananda Chakraborty, Ghazala Javed, Asim Ali Khan

Email(s): alokananda.lahiri@gmail.com

DOI: 10.52711/0974-360X.2024.00440   

Address: Priyanka Pallapolu1, Lahari Kuna1, Alokananda Chakraborty1*, Ghazala Javed2, Asim Ali Khan2
1National Research Institute of Unani Medicine for Skin Disorders, ESI x Road, Hyderabad, Telangana, India.
2Central Council for Research in Unani Medicine (CCRUM), New Delhi, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Introduction: Gastritis, often known as hyperacidity, is a prevalent issue among many people. In Unani Medicine, the disorders associated with stomach (Amraze Meda) are thoroughly documented and it is associated with the Choleric temperament. Oxidative stress is a significant factor to the onset of several gastrointestinal disorders. In the present research CYP3A4 and GST genetic polymorphisms are assessed in gastritis patients with Safrawi (Choleric) temperament. Materials and methods: A total of 200 subjects of which 100 gastritis patients and 100 control subjects with Safrawi (Choleric) temperament were recruited. DNA was isolated and analyzed for CYP3A4 and GST genetic polymorphisms by PCR and RFLP methods. Results: CYP3A4 CC genotype, GSTT1 and GSTM1 null genotypes were predominantly associated with gastritis risk in Safrawi (Choleric) temperament subjects with p<0.001. CYP3A4 recessive model (OR=3.27, 95% CI 1.02-10.52, p < 0.033) was significantly associated with disease susceptibility. In combined analysis CYP3A4 –TT and GST Null (-) were significantly allied with 5.68-fold risk CYP3A4 –TT and GSTT1 Null (-) were significantly allied with 5.68-fold risk whereas CYP3A4 –TT, TC, CC and GSTM1 Null (-) showed significant association with 18.81, 11.68 and 15.57folds risk to develop gastritis. In haplotype analysis, T-GSTT1 (-) -GSTM1 (-), T-GSTT1 (+)-GSTM1 (-) and C-GSTT1 (-)-GSTM1 (-) haplotypes showed significant association with increased risk towards disease susceptibility, corroborating the hypothesis that these SNPs serve a stimulatory role in illness development in Safrawi (Choleric) temperament. Discussion and conclusion: Proper diet and a healthy lifestyle can help prevent gastritis in its early stages. CYP3A4 (TC and CC), GSTT1null and GSTM1null genotypes appear to enhance the risk of gastritis in Safrawi (Choleric) temperament individuals. To reduce illness propensity and reverse pathogenic processes, early diagnosis of these genetic variations is necessary.


Cite this article:
Priyanka Pallapolu, Lahari Kuna, Alokananda Chakraborty, Ghazala Javed, Asim Ali Khan. Association of CYP3A4, GSTT1 and GSTM1 Gene Polymorphisms in early prediction of Gastritis in Choleric Temperament as per Unani Philosophy in Indian population. Research Journal of Pharmacy and Technology. 2024; 17(6):2804-2. doi: 10.52711/0974-360X.2024.00440

Cite(Electronic):
Priyanka Pallapolu, Lahari Kuna, Alokananda Chakraborty, Ghazala Javed, Asim Ali Khan. Association of CYP3A4, GSTT1 and GSTM1 Gene Polymorphisms in early prediction of Gastritis in Choleric Temperament as per Unani Philosophy in Indian population. Research Journal of Pharmacy and Technology. 2024; 17(6):2804-2. doi: 10.52711/0974-360X.2024.00440   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-59


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