Author(s): Nasrudin, Wahyono, Mustofa, Ratna Asmah Susidarti

Email(s): nasrud_la@uho.ac.id

DOI: 10.52711/0974-360X.2024.00429   

Address: Nasrudin1*, Wahyono2, Mustofa3, Ratna Asmah Susidarti4
1Faculty of Pharmacy Halu Oleo University, Kendari, Indonesia, 93232.
2Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
3Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Senggugu is reported to contain potent antioxidant compounds. This study aims to determine the antioxidant activity, to isolate and to characterize the structure of antioxidant compounds of methanolic extract of senggugu’s root bark. The ground dried root bark was macerated gradually with n-hexane, ethyl acetate and methanol. The methanol extract was evaluated for the antioxidant activity using DPPH radical scavenging method and its antioxidant activity was compared to rutin (positive control). Isolation of the antioxidant compound was performed using preparative thin layer chromatography over silica gel GF254 eluted with a mixture of ethyl acetate: acetone: formic acid: water (5:3:1:1). The active compound was obtained as needle like crystals and the structure was characterized by spectroscopic methods as kojic acid which has never been reported as a constituent of this plant. Methanol extract showed to have DPPH scavenger activity with the IC50 12.63±0.09µg/mL, weaker than rutin (IC50=3.49±0.006µg/mL). Kojic Acid is a new compound from the methanol extract of senggugu’s root bark which has very strong antioxidant activity.


Cite this article:
Nasrudin, Wahyono, Mustofa, Ratna Asmah Susidarti. Antioxidant activity of Methanol extracts and the Isolation of new compounds from Senggugu’s root bark (Clerodendrum serratum L.Moon). Research Journal of Pharmacy and Technology. 2024; 17(6):2739-2. doi: 10.52711/0974-360X.2024.00429

Cite(Electronic):
Nasrudin, Wahyono, Mustofa, Ratna Asmah Susidarti. Antioxidant activity of Methanol extracts and the Isolation of new compounds from Senggugu’s root bark (Clerodendrum serratum L.Moon). Research Journal of Pharmacy and Technology. 2024; 17(6):2739-2. doi: 10.52711/0974-360X.2024.00429   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-48


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