Author(s): Pravesh Kumar Sharma, Ashutosh Pareek, Mahendra Singh Ashawat, Sidhraj Singh Sisodia

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DOI: 0.52711/0974-360X.2024.00410   

Address: Pravesh Kumar Sharma1,2*, Ashutosh Pareek2, Mahendra Singh Ashawat3, Sidhraj Singh Sisodia4
1Department of Pharmacy, Vivekananda Global University, Jaipur 303012, India.
2Department of Pharmacy, Banasthali Vidyapith, Banasthali 304022, India.
3Laureate Institute of Pharmacy, Kathog, India.
4BN College of Pharmacy, Bhupal Nobles' University, Udaipur 313001, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024

Objective: This study aimed to assess the antioxidant activity of the hydroalcoholic leaf extract of Gossypium herbaceum Linn. in Swiss albino mice subjected to carbon tetrachloride (CCl4) treatment. Materials and Methods: Oxidative stress was induced in mice through the subcutaneous administration of CCl4 in a liquid paraffin suspension (1:2 v/v) every other day for one week at a dosage of 1 ml/kg body weight. An assessment of hepatoprotective effects was performed through the analysis of serum enzymes superoxide dismutase (SOD), glutathione (GSH), and supermalondialdehyde (MDA). The impact of administering Gossypium herbaceum Linn. at 50, 100, and 200 mg/kg p.o. on these parameters was investigated. Histological examination of liver sections complemented these biochemical assessments, with silymarin as the positive control. Results: CCl4 administration led to a reduction in SOD, GSH, and CAT levels and a significant increase in MDA levels, indicating oxidative degeneration. Gossypium herbaceum Linn. administration (at doses of 50, 100, and 200 mg/kg p.o.) significantly elevated antioxidant enzyme levels, including SOD, catalase, and reduced glutathione, while concurrently reducing malondialdehyde levels responsible for tissue lipid peroxidation. Histological analysis of liver sections revealed hepatic regeneration following Gossypium herbaceum Linn. administration, with results comparable to the standard drug, silymarin. Conclusion: The findings suggest that G. herbaceum Linn. possesses substantial antioxidant potential against CCl4-induced oxidative stress in mice models in a dose-dependent manner.

Cite this article:
Pravesh Kumar Sharma, Ashutosh Pareek, Mahendra Singh Ashawat, Sidhraj Singh Sisodia. Protective effect of Gossypium herbaceum Linn. leaf extract on carbon tetra chloride induced oxidative stress in mice. Research Journal of Pharmacy and Technology. 2024; 17(6):2623-7. doi: 0.52711/0974-360X.2024.00410

Pravesh Kumar Sharma, Ashutosh Pareek, Mahendra Singh Ashawat, Sidhraj Singh Sisodia. Protective effect of Gossypium herbaceum Linn. leaf extract on carbon tetra chloride induced oxidative stress in mice. Research Journal of Pharmacy and Technology. 2024; 17(6):2623-7. doi: 0.52711/0974-360X.2024.00410   Available on:

1.    Gitte SJ, Xianli WU, Kelly MP, Janelle B, Steve GC, Larry S, Robert B, John RE, Alexander GS. In-vitro and In-vivo Antioxidant and Anti-inflammatory capacities of an Antioxidant-Rich Fruit and Berry Juice Blend. Results of a Pilot and Randomized, Double-Blinded, Placebo-controlled, Crossover Study. J. Agric Food Chem. 2008; 56: 8326-8333.
2.    Soni H, Nayak G, Mishra K, Singhai AK, Pathak AK. Evaluation of Phyto Pharmaceutical and Antioxidant Potential of Methanolic Extract of Peel of Punica granatum. Res. J. Pharm. Tech. 2010; 3(4): 1170-1174.
3.    Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative disease of aging. Proceedings. Natl acad Sci. 1993; 90: 7915-7922.
4.    Vijayakumar K, Anand AV. Protective Effects of Psidium guajava and its Isolated Fraction on CCl4 Induced Oxidative Stress. Res. J. Pharm. Tech. 2016; 9(8): 1155-1160. doi: 10.5958/0974-360X.2016.00220.1
5.    Rajesh M.G., Latha M.S. Antioxidant Potential of the Flowers of Woodfordia fruticosa (L.) Kurz. in Albino Rats. Res. J. Pharmacog Phytochem. 2013; 5(3): 127-129.
6.    Jadhav HR, Bhutani KK. Antioxidant Properties of Indian Medicinal Plants. Phytother Res. 2002; 6: 771-73.
7.    Chakraborty P, Kumar S, Dutta D, Gupta V. Role of Antioxidants in Common Health Diseases. Res J. Pharm. Tech. 2009; 2 (2): 238-244.
8.    Wang D, Wang L, Zhu F, Zhu J, Chen XD, Zou L, Saito M, Li L. In vitro and in vivo studies on the antioxidant activities of the aqueous extracts of Douchi (a traditional Chinese salt-fermented soybean food). Food Chem. 2008; 107: 1421-1428.
9.    Sharma PC, Yenle MB, Dennis TJ. Data Base of Medicinal Plants Used in Ayurveda. Central council for Research in Ayurveda and Siddha, Department of Indian system of Medicine and Homoeopathy, Ministry of Health and Family Welfare, Govt of India. 2005; 2: 69-88.
10.    Indian medicinal plants, a compendium of 500 species. 3rd ed. Orient Longman LTD 2001. 100-103.
11.    Garratt LC, Janagoudar BS, Anthony P, Davey MR, Power JB, Lowe KC. Hemoglobin-stimulated growth and antioxidant activities in cultured cotton cells. Free Radic Biol Med. 2001; 31:1156-62.
12.    Lee R, Lin JY. Antimutaganic  activity  of  extracts  from anticancer  drugs  in  chinese  medicine. Mutation Res 1988; 204 (2):229-234.
13.    Lin GZ. Clinical study of Gossypol as a male contraceptive. Reproduction 1981; 5(3): 189-193.
14.    Reddy UM, Reddy MM. Antibacterial activity of leaf extract of Gossypium herbaceum. Geo Bios. 1981; 8(6): 277-278.
15.    Hebber SS, Harsha VH, Shri  Patni  V,  Hegde  GR.  Ethno medicine of Dharwad district in Karnataka, India: Plants used in oral health care. J. Ethnopharmacol. 2004; 94: 261-266.
16.    Quereshi AA, Prakash T, Patil T, Swamy AHMV, Gouda AV. Hepatoprotective and antioxidant activities of  flowers of Calotropis procera ( Ait ) R. Br. In CCl4 induced hepatic damage. Indian J. Exp. Biol. 2007; 45: 304-310.
17.    Sisodia SS, Bhatnagar M. Hepatoprotective activity of Eugenia jambolana Lam. In carbon tetrachloride treated rats. Indain J. Pharmacol. 2009; 41: 23-27.
18.    Patel PK, Sahu J, Prajapati NK, Dubey BK, Alia A. Hepatoprotective Effect of Ethanolic and Hydro Alcoholic Leaf Extract of Madhuca indica in Carbon Tetra Chloride Intoxicated Rat. Res. J. Pharmacol Pharmacodyn. 2012; 4(5): 311-314.
19.    Tiwari P, Kumar K, Panik R, Pandey A, Pandey A, Sahu PK. Hepatoprotective Potentials of Butea monosperma Stem Bark Extract against Carbon Tetrachloride Induced Hepatotoxicity in Albino Rats. Res. J. Pharmacol Pharmacodyn. 2011; 3(5): 281-284.
20.    Aghel N, Rashidi I, Mombeini A. Hepatoprotective activity of Capparis spinosa root bark against CCl4 induced hepatic damage in Mice. Iran J Pharm Res. 2007; 6: 285-290.
21.    Winterbourn CC, Hawkins RE, Brain M, Carrel RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975; 85: 337-341.
22.    Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389.
23.    Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1952; 82: 70.
24.    Buege JA, Aust SD. The thiobarbituric acid assay. Methods in Enzymology. 1978; 52: 306.
25.    Mukhergee KL. Laboratory techniques in histology, In Medical Laboratory Technology. 7th ed. New Delhi: Tata McGraw-Hill; 1993: 3. 1124-1157.
26.    Li JC, Peng L, Sun BH, Ikeda T, Nohara T. Hepatoprotective activity of the constituents in Swertia pseudochinesis. Biol Pharm Bull. 2000; 28: 534-537.
27.    Mahajan BK. Methods in Biostastics. 5th ed. New Delhi: Medical Publishers (P) Ltd. 129-153.
28.    Recknagel RO. A new direction in the study of carbon tetrachloride hepatptoxicity. Life Sci. 1983; 33:401.
29.    Shah VV, Shah VK, Patel KN, Sheth NR, Patel MM. Improvement on the Carbon Tetrachloride inducing Hepatotoxicity in rats using Ipomoea turpethum Root. Res. J. Pharm Phytochem. 2017; 9(4): 231-234. doi: 10.5958/0975-4385.2017.00043.7
30.    Chidambara MA, Vanita J, Rajesh M, Mahadeva PRS, Ravishankar GA. In vivo antioxidant activity of carotenoids from Dunaliella salina- a green microalga. Life Sci. 2005; 76:1381.
31.    Vijayabhaskar K, Venkateshwarlu G, Bhaskar J, Srisailam K, Swapna M. Antioxidant and Hepatoprotective Effects of the Methanol Extract of the Flowers of Tamarindus indica. Asian J. Pharm. Tech. 2011; 1(3): 73-78.
32.    Mc Cord JM and Fridovich I. Superoxide dismutase, an enzymatic function for erythrocuprein. J Biol Chem. 1969; 224: 6049.
33.    Castro JA, Ferrya GC, Castro CR, Sasamett OMF, Gillete JR. Prevention of carbon tetrachloride induced necrosis by inhibitors of drug metabolism: Further studies on the mechanism of their action. Biochem. Pharmacol. 1974; 23: 295.
34.    Patil SA, Joshi VG, Sambrekar SN, Desai NS. Hepatoprotective and Antioxidant Potential of Extracts from Whole Plant of Solanum surattense Burm. Res J Pharm Phytochem. 2011; 3(1): 17-21.
35.    Rajmohan T and Anthony LL. Hepatoprotective and antioxidant effect of tender coconut water on carbon tetrachloride induced liver injury in rats. Indian. J. Biochem. Biophys. 2003; 40: 354.
36.    Bhakta T, Mukherjee P, Kakali K. Evaluation hepatoprotective activity of Cassia fistula leaf extract. J Ethanopharmacol. 1999; 66: 227.
37.    Sapakal VD, Shikalgar TS, Ghadge RV, Adnaik RS, Naikwade NS, Magdum CS. In vivo screening of Antioxidant profile: A Review. J.  Herbal Med. Toxicol. 2008; 2:1-8.
38.    Samal PK. Antioxidant activity of Gloriosa superba in albino rats. Res. J. Pharm Phytochem. 2013; 5(6):288-292
39.    Suneetha B, Prasad KVSRG, Nishanthi PD, Soumya BR, Kumar BS. Hepatoprotective and Antioxidant Activity of Methanolic Extract of Actinodaphne madraspatana against Carbon tetrachloride Induced Hepatotoxicity. Res. J. Pharm Phytochem. 2014; 6(4): 176-180.

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