Author(s): Aishwarya S. Patil, Mayuri A. Tupe, Devendra S. Shirode


DOI: 10.52711/0974-360X.2024.00401   

Address: Aishwarya S. Patil, Mayuri A. Tupe, Devendra S. Shirode*
Department of Pharmacology, Dr. D.Y. Patil College of Pharmacy, Dr. D.Y. Patil Educational Complex, Sector 29, Pradhikaran Akurdi, Pune, Maharashtra, 411044.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024

Inflammation is body’s defence mechanism. There are various inflammatory and pro-inflammatory factors released in blood after injury. Prevalence of diseases associate with chronic inflammation will increase persistently in next 30 years. 3 in 5 people died with inflammation. Currently various synthetic treatments available but have many side effects. In this view, present study was designed to prepare a promising anti-inflammatory treatment from natural source. Anti-inflammatory effect of polyherbal formulation [prepared from ethanolic extracts of Hugonia mystax and Blumea lacera (1:1)] was evaluated against carrageenan and formalin induced paw oedema. Preliminary phytochemical screening revealed that phenolic acids, flavonoids, tannins, present in formulation. Acute oral toxicity was performed as per OECD guideline, 200 mg/kg and 400mg/kg was selected as lower dose and higher dose respectively. Paw oedema and paw thickness were assessed in all groups. Paw oedema and paw thickness increase in disease control group and significantly decrease in all treatment groups. It is concluded that polyherbal formulation have anti-inflammatory potential due to polyphenols, saponin and terpenoids. Further potent compounds can be isolated for preparation of formulation and clinical trials may be performed.

Cite this article:
Aishwarya S. Patil, Mayuri A. Tupe, Devendra S. Shirode. Anti-inflammatory effect of Polyherbal Formulation. Research Journal of Pharmacy and Technology. 2024; 17(6):2568-2. doi: 10.52711/0974-360X.2024.00401

Aishwarya S. Patil, Mayuri A. Tupe, Devendra S. Shirode. Anti-inflammatory effect of Polyherbal Formulation. Research Journal of Pharmacy and Technology. 2024; 17(6):2568-2. doi: 10.52711/0974-360X.2024.00401   Available on:

1.    Luciano Silva. A Literature Review of Inflammation and Its Relationship with the Oral Cavity, 2016; 2(1): 1-7. doi: 10.17352/2455-5363.000006.
2.    Adami JG. Inflammation: An Introduction to the Study of Pathology, 1909.
3.    David D. Chaplin MD. Overview of the immune response. Journal of Allergy, and Clinical Immunology. 2010; 3-23:
4.    Ning Xu Lande´n, Dongqing Li, Mona Stahle. Transition from inflammation to proliferation: a critical step during wound healing, 2016, 73: 3861–3885, doi 10.1007/s00018-016-2268-0.
5.    Mohamed Eddouks, Debprasad Chattopadhyay. Animal Models as Tools to Investigate Antidiabetic and Anti-Inflammatory Plants, 2012, Hindawi Publishing Corporation, 14 pages, doi:10.1155/2012/142087.
6.    Bennett JM, Reeves G, Billman GE and Sturmberg JP. Inflammation–Nature’s Way to Efficiently Respond to All Types of Challenges: Implications for Understanding and Managing “the Epidemic” of Chronic Diseases. 2018; 5: 316, doi: 10.3389/fmed.2018.00316.
7.    Sugimoto MA, Sousa LP, Pinho V, Perretti M and Teixeira MM. Resolution of Inflammation: What Controls Its Onset. 2016; 7: 160, doi: 10.3389/fimmu.2016.00160.
8.    Kalaichelvi S. Dharshini V. Kalaimony RK, Sivarangini S. Medicinal uses and Pharmacological activity of Mothirakanni (Hygonia mystax Linn,) - A review. 2015, 2.
9.    D. Venkatanarayana, P. Umamaheshwar. A Review On Herbal Antioxidant Agents. 2019; 10(4): 6932 – 6942.
10.    Vasuki B, Vijayabaskaran M and Sambath Kumar R. Anti-Inflammatory activity of ethanol extract of Hugonia mystax Leaves. 2016; 5(4):  227-229.
11.    G. Rajeswari. Anti-Inflammatory Activity of Leaf And Bark of Hugonia Mystax L. (Linaceae). 2013; 2(2): 80-83.
12.    Shirode DS. Jain BB, Mahendra Kumar. Evaluation of hepatoprotective effect of Hugonia mystax leaves in CCl4 induced liver damage in rats. 2015; 5(1): 587-592.
13.    Shirode DS. Jain BB. Mahendra Kumar. In Vivo Antioxidant And Hepatoprotective Effects of Hugonia Mystax In Paracetamol Induced Hepatotoxicity In Rats. 2015; 8: 291-4, Https://Journals.Innovareacademics.In/Index.Php/Ajpcr/Article/View/7489.
14.    Shirode DS. Sonawane C. Powar P. Hugonia mystax: A Review. 2017; 4 (2): 1-3.
15.    Shirode DS. Powar PV. Sadar SS and Jain BB. Effect of Hugonia Mystax Leaves on Physical and Biochemical Parameters in Ethanol Induced Liver Damage in Rats. 2019; 1(6): 160-163.
16.    Shirode DS. Powar PV. Chaudhari SP. Jain BB. Effect of Hugonia mystax Leaves on Histopathological Studies, Tissue Glutathione and Lipid Peroxide Levels of Rat Liver. 2019; 4(11): 809-813.
17.    Shirode DS. Jain B. Kumar CB. Effect of ethanol extract of Hugonia mystax on Rifampicin induced hepatotoxicity in rats. 2015; 8(4): 651- 655.
18.    Shirode D. Jain B. and Agarwal A. Evaluation of The Anti-Ulcer Activity of Ethanolic Extract of Leaves of Hugonia Mystax. 2021; 12(1): 28-31.
19.    Abdul Mukit Barbhuiya, S. Sindhura, N. Chenna Keshavulu, Evaluation of Stroke Preventive Activity of Hugonia mystax Extract in Rats. 2016; 1: 18-29.
20.    B Singh, S Singh, Surender Singh, L Ali, Ritu Mahajan. Ethnobotanical Significance and Antimicrobial Activity of Blumea lacera (Roxb.) DC. 2010; 1(3): 314 – 316.
21.    Shirode DS. Kulkarni AV. Jain BB. Effect of Blumea lacera on tissue GSH, lipid peroxidation and hepatic cells in ethanol induced hepatotoxicity in rats. 2019; 11 (12): 46-50.
22.    Shirode DS. Powar PV. Sadar SS and Jain BB. Antiulcer effect of Blumea lacera against Gastric ulcers in rats. 2020; 3(7): 334.-3342, doi:10.5958/0974-360X.2020.00592.2
23.    Shirode DS. Bindurani LGP Ram. Jain G. Singh A. Protective Effect of Blumea lacera in Rifampicin Induced Hepatotoxicity in Rats. 2021; 33(46B): 555-561.
24.    Mokat DN. Torawane SD. Suryawanshi YC. Chemical profiling of two aromatic weeds, Cyathocline purpurea and Blumea lacera. 2020; 11: 205-210 doi: 10.25081/cb.2020.v11.6259.
25.    Laakso T. Laakso S. Hiltunen R. Composition of the Essential Oil of Blumea lacera DC. (Asteraceae) Leaves from Nigeria. 1989; 4:73-75.
26.    Yadav VK, Irchhiaya R. Ghosh AK. Phytochemical and Pharmacognostical studies of Blumea lacera. 2018; 12 (1): 140-148.
27.    Mishra P. Irchhiaya R, Mishra SK. Phytochemical investigation and spectral studies of isolated flavonoid from ethanolic extract of whole plant Blumea lacera D.C. 2015; 4(2): 01-04.
28.    Rao AM. In vitro antioxidant, and anticancer activity of Blumea lacera leaf extract. 2021; 12: 168-176.
29.    Meshram GG, Kumar A, Rizvi W. Tripathi CD. Khan RA.  Evaluation of the anti-inflammatory activity of the aqueous and ethanolic extracts of the leaves of Albizzia lebbeck in rats. 2016; 6: 172-175.
30.    Singh M. Kumar V. Singh I. Anti-inflammatory activity of aqueous extract of Mirabilis Jalapa Linn. Leaves. 2010; 2 (6): 364-367.
31.    Kumar T. Jain V. Antinociceptive and Anti-Inflammatory Activities of Bridelia retusa Methanolic Fruit Extract in Experimental Animals, 2014. Hindawi Publishing Corporation, 12 pages;
32.    Arzi A. Olapour S. Yaghooti H. Karampour NS. Effect of Royal Jelly on Formalin Induced-Inflammation in Rat Hind Paw. 2015; 10(1): doi: 10.17795/jjnpp-22466.
33.    Damas J. Liégeois. JF. The inflammatory reaction induced by formalin in the rat paw. 1999; 220–227,

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