Author(s): Riska Surya Ningrum, Elga Renjana, Aisyah Hadi Ramadani, Yudhi Dwi Kurniawan, Mahani, Oktan Dwi Nurhayat

Email(s): , , , , ,

DOI: 10.52711/0974-360X.2024.00395   

Address: Riska Surya Ningrum1, Elga Renjana2, Aisyah Hadi Ramadani3, Yudhi Dwi Kurniawan4, Mahani5, Oktan Dwi Nurhayat6
1Research Center for Biomass and Bioproducts, National Research and Innovation Agency, Jl. Raya Bogor Km.46 Cibinong, West Java, Indonesia.
2Research Center for Applied Botany, National Research and Innovation Agency, Jl. Raya Bogor Km.46 Cibinong, West Java, Indonesia.
3Department of Biology, Faculty of Science, Technology and Education, University of Muhammadiyah Lamongan, Jl. Plalangan, Wahyu, Plosowahyu, Lamongan, East Java, Indonesia.
4Research Center for Pharmaceutical Ingredient and Traditional Medicine, National Research and Innovation Agency, Jl. Raya Bogor Km.46 Cibinong, West Java, Indonesia.
5Food Technology Department, Faculty of Agric Industrial Technology, Padjadjaran University, West Java, Indonesia.
6Research Center for Applied Microbiology, National Research and Innovation Agency, Jl. Raya Bogor Km.46 Cibinong, West Java, Indonesia.

Published In:   Volume - 17,      Issue - 6,     Year - 2024

Propolis, an extremely sticky and resinous substance collected by honeybees, has been widely used as a health food, antioxidant, and antimicrobial. Utilization of propolis as nutraceutical depends on the bioactive compounds contained therein. The plant source, type of bees, and region of honeybees are the main factors affecting the chemical composition of the bioactive compounds in propolis. This study aims to determine the bioactive compounds in propolis from Wallacetrigona incisa using LC-MS/MS and to analyze their antibacterial activity by in vitro and molecular docking approach. A series of propolis with different concentration (5, 7.5, 10, 20, 30, and 100 % w/v) were tested against five bacteria (P. acnes, S. aureus, S. epidermidis, B. subtilis, and E. coli) using disk diffusion method. The inhibition mechanism against the bacteria was studied by molecular docking approach. For the LC-MS/MS analysis, seven bioactive compounds were detected in the propolis from W. incisa: ganoderic acid R, mulberranol, schizandrin A (deoxyschizandrin), neoquassin, octahydrocurcumin, isorhamnetin, and 2-methoxyanofinic acid. Moreover, for the antibacterial activity, propolis has strong inhibition at concentration of 30% and 100%, and better efficacy on Gram-positive bacterial species (S. epidermidis, B. subtilis, S. aureus, P. acnes) than gram-negative bacterial (E. coli). Ganoderic acid R and mulberranol were found to be the most potential bioactive compounds of the propolis as antibacterial agents due to their good performance in interacting with target proteins of bacteria.

Cite this article:
Riska Surya Ningrum, Elga Renjana, Aisyah Hadi Ramadani, Yudhi Dwi Kurniawan, Mahani, Oktan Dwi Nurhayat. Bioactive Compounds in Propolis from Wallacetrigona incisa and Their Application as Antibacterial Agent: In vitro and Molecular Docking Approach. Research Journal of Pharmacy and Technology. 2024; 17(6):2522-0. doi: 10.52711/0974-360X.2024.00395

Riska Surya Ningrum, Elga Renjana, Aisyah Hadi Ramadani, Yudhi Dwi Kurniawan, Mahani, Oktan Dwi Nurhayat. Bioactive Compounds in Propolis from Wallacetrigona incisa and Their Application as Antibacterial Agent: In vitro and Molecular Docking Approach. Research Journal of Pharmacy and Technology. 2024; 17(6):2522-0. doi: 10.52711/0974-360X.2024.00395   Available on:

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