Author(s): Kavitha E, Srikumar R

Email(s): kavitha@svmchrc.ac.in

DOI: 10.52711/0974-360X.2024.00391   

Address: Kavitha E1*, Srikumar R2
1Assistant Professor, Department of Microbiology, Sri Venkateshwaraa Medical College Hospital and Research Centre, Puducherry, India.
2Associate Professor, Department of Microbiology, Centre for Research, Sri Lakshmi Narayana Institute of Medical Sciences and Research Centre, Puducherry, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Introduction: Methicillin resistance is exhibiteddue to presence of mecA gene and is commonly referred as SCCmec types in MRSA. SCCmec typing helps and acts a way to characterize MRSA infections and gain knowledge in its epidemiology. Our study aims to characterize mecA gene responsible in methicillin resistance by SCCmec typing and emphasize the use of SCCmec typing in routine diagnosis for the classification of MRSA. Methods: A total of 191 clinical isolates were included for the study.Documentation like clinical details and associated risk factors for MRSA acquisition were done. SCCmec typing (type I-V) was done by using M-PCR as per Boyeet al., 2007 protocol. Results: In our study, five different SCCmec types namely SCCmec type I, type II, type III, type IV and type V were detected among the clinical isolates of MRSA. Sccmec type V (53%) was found to be the most predominant type followed by SCCmec type III (47%) and SCCmec type IV (37.6%). Among the 191 MRSA isolates, 7 clinical isolates were SCCmec non-typeable isolates. Conclusion: Our study reports existence of multiple SCCmec types among the MRSA clinical isolates. SCCmec typing is helpful in determining the contribution of the isolates in understanding their association being CA-MRSA or HA-MRSA which in turn helps in designing effective surveillance and control strategies in the management of MRSA infections.


Cite this article:
Kavitha E, Srikumar R. SCC mec typing among the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) from a Tertiary Care Hospital, Puducherry. Research Journal of Pharmacy and Technology. 2024; 17(6):2501-4. doi: 10.52711/0974-360X.2024.00391

Cite(Electronic):
Kavitha E, Srikumar R. SCC mec typing among the clinical isolates of Methicillin Resistant Staphylococcus aureus (MRSA) from a Tertiary Care Hospital, Puducherry. Research Journal of Pharmacy and Technology. 2024; 17(6):2501-4. doi: 10.52711/0974-360X.2024.00391   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-10


REFERENCES:
1.    Shrestha B, Pokhrel BM, Mohapatra TM. Antibiotic susceptibility pattern of nosocomial isolates of Staphylococcus aureus in a tertiary care hospital, Nepal. Journal of the Nepal Medical Association. 2009; 48(175): 234-8. https://pubmed.ncbi.nlm.nih.gov/20795464/.
2.    Ramalingam AJ. History of antibiotics and evolution of resistance.Research Journal of Pharmacy and Technology. 2015; 8(12): 1719-24. https://bit.ly/49X5bIl.
3.    Khushboo K, Saloni B, Singh RK. A briefing of a global crisis: antibiotic resistance. Asian Journal of Research in Pharmaceutical Sciences. 2020; 10(4): 264-272. https://doi.org/10.5958/2231-5659.2020.00047.8.
4.    Niyas FM, Gopinath P. Comparative study on detection of MRSA using oxacillin agar screening method, cefoxitin disc diffusion method and mecAgene by PCR among clinical isolates of Staphylococcus aureus. Research Journal of Pharmacy and Technology. 2016; 9(9): 1317-20. http://dx.doi.org/10.5958/0974-360X.2016.00250.X.
5.    Boye K, Bartels MD, Andersen IS, Moeller JA, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clinical Microbiology and Infection. 2007; 13(7): 725-727. https://doi.org/10.1111/j.1469-0691.2007.01720.x.
6.    Nagasundaram N, Sistla S. Existence of multiple SCCmec elements in clinical isolates of methicillin-resistant Staphylococcus aureus. Journal of Medical Microbiology. 2019; 68(5): 720-727. https://bit.ly/48UjJI5.
7.    Amberpet R, Sistla S, Sugumar M, Nagasundaram N, Manoharan M, Parija SC. Detection of heterogeneous vancomycin-intermediate Staphylococcus aureus: a preliminary report from south India. The Indian Journal of Medical Research. 2019; 150(2): 194-198. 10.4103/ijmr.IJMR_1976_17.
8.    Abimannan N, Sumathi G, Krishnarajasekhar OR, Sinha B, Krishnan P. Clonal clusters and virulence factors of methicillin-resistant Staphylococcus aureus: Evidence for community-acquired methicillin-resistant Staphylococcus aureus infiltration into hospital settings in Chennai, South India. Indian Journal of Medical Microbiology. 2019; 37(3): 326-336, https://doi.org/10.4103/ijmm.IJMM_18_271.
9.    Bezlon G, Shanmugha SD, Rinu ER. Design and Stabilization of Natural Antibacterial Compound Allicin against Methicillin-Resistant Staphylococcus aureus for Treatment as a Novel Antibiotic. Research Journal of Engineering and Technology. 2013; 4(4): 179-81, https://bit.ly/3V2TI5L.
10.    Kavitha E, Srikumar R, Muthu G. Inducible clindamycin resistance among clinical isolates from a tertiary care hospital. Research Journal of Pharmacy and Technology. 2018; 11(11): 5008-12. http://dx.doi.org/10.5958/0974-360X.2018.00913.7.
11.    Fasihi Y, Kiaei S, Kalantar-Neyestanaki D. Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran. Journal of Epidemiology and Global Health. 2017; 7(4): 263-267. https://doi.org/10.1016/j.jegh.2017.08.004.
12.    Vyas A, Singh K, Kumar G. Prevalence and drug resistance among bacteria of urinary tract infections in females in Punjab, India. Research Journal of Pharmacy and Technology. 2017; 10(2): 575-8. http://dx.doi.org/10.5958/0974-360X.2017.00114.7.
13.    Lawung R, Chuong LV, Cherdtrakulkiat R, Srisarin A, Prachayasittikul V. Revelation of staphylococcal cassette chromosome mec types in methicillin-resistant Staphylococcus aureus isolates from Thailand and Vietnam. Journal of microbiological methods. 2014; 107: 8-12. https://doi.org/10.1016/j.mimet.2014.08.024.
14.    Wang CC, Lo WT, Chu ML, Siu LK. Epidemiological typing of community-acquired methicillin-resistant Staphylococcus aureus isolates from children in Taiwan. Clinical Infectious Diseases. 2004; 39(4): 481-487. https://doi.org/10.1086/422642.
15.    Mert G, Kiliç A, Bedir O, Başustaoğlu AC. Clinical significance and staphylococcal cassette chromosome mec (SCCmec) characterization of coagulase-negative staphylococci isolated from blood cultures. Turkish Journal of Medical Sciences. 2011; 41(5): 859-865. 10.3906/sag-1009-1138.
16.    Hashemizadeh Z, Hadi N, Mohebi S, Kalantar-Neyestanaki D, Bazargani A. Characterization of SCCmec, spa types and Multi Drug Resistant of methicillin-resistant Staphylococcus aureus isolates among inpatients and outpatients in a referral hospital in Shiraz, Iran. BMC Research Notes. 2019; 12(1): 1-6. https://bit.ly/4a0P0df.
17.    Umamageswari SS, George P, Kalyani M. A study on coexistence of panton valentine leukocidin gene from hospital acquired methicillin resistance Staphylococcus aureus. Research Journal of Pharmacy and Technology. 2019; 12(2): 508-12. http://dx.doi.org/10.5958/0974-360X.2019.00089.1.
18.    Machado A B M P, Reiter K C, Paiva R M, Barth A L. Distribution of staphylococcal cassette chromosome mec (SCCmec) types I, II, III and IV in coagulase-negative staphylococci from patients attending a tertiary hospital in southern Brazil. Journal of Medical Microbiology. 2007; 56(10): 1328-1333. https://bit.ly/3T9hbQu.
19.    Monecke S, Slickers P, Gawlik D, Müller E, Reissig A, Ruppelt-Lorz A, Akpaka PE, Bandt D, Bes M, Boswihi SS, Coleman DC. Molecular typing of ST239-MRSA-III from diverse geographic locations and the evolution of the SCCmec III element during its intercontinental spread. Frontiers in Microbiology. 2018; 9: 1-31. https://doi.org/10.3389/fmicb.2018.01436.
20.    Sarhan SR, Mohammed HA. Isolation and Identification of mecA gene from MRSA isolated from Local Sheep and Evaluate the Inhibitory effect of Cranberry Leaves extract In-Vitro. Research Journal of Pharmacy and Technology. 2019; 12(5): 2131-6. http://dx.doi.org/10.5958/0974-360X.2019.00353.6.
21.    Azeez S, Panakkal LM, Meenpidiyil SS, Sulaiman N. Impact of Clinical Pharmacist Intervention in promoting Rational Antibiotic use in Pediatric patients. Research Journal of Pharmacy and Technology. 2020; 13(11): 5077-82. 10.5958/0974-360X.2020.00889.6.
22.    Rajesh KS, Honey VS, D'souza UP, Sharma R, Raj KC. Study of Antibiotic resistance pattern in uropathogens at a Tertiary Care Hospital. Research Journal of Pharmacy and Technology. 2020; 13(3): 1253-6. http://dx.doi.org/10.5958/0974-360X.2020.00231.0.

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