Author(s): Kovaleva T.Yu., Bokov D.O., Sergunova E.V., Bobkova N.V., Kovalev I.S., Chernova S.V., Nesterova N.V., Pavlenko A.A., Dorovskikh E.A., Bessonov V.V., Luferov A.N.

Email(s): kovaleva_t_yu@staff.sechenov.ru

DOI: 10.52711/0974-360X.2024.00382   

Address: Kovaleva T.Yu.1*, Bokov D.O.1,2, Sergunova E.V.1, Bobkova N.V.1, Kovalev I.S.3, Chernova S.V.1, Nesterova N.V.4, Pavlenko A.A.1, Dorovskikh E.A.1, Bessonov V.V.2, Luferov A.N.1 1Sechenov First Moscow State Medical University, 8 Trubetskaya St., bldg. 2, Moscow, 119991, Russian Federation.
2Federal Research Center of Nutrition, Biotechnology and Food Safety, 2/14 Ustyinsky Pr., Moscow, 109240, Russian Federation.
3Moscow State Academy of Veterinary Medicine and Biotechnology Named After K.I. Skryabin, 23 Academician Skryabin St., Moscow, 109472, Russian Federation. 4Moscow University for Industry and Finance “Synergy” (Synergy University), 80 Leningradsky prospect, Moscow, 125190, Russian Federation.
*Corresponding Author

Published In:   Volume - 17,      Issue - 6,     Year - 2024


ABSTRACT:
Objective: Sour cherry fruits (Cerasus vulgaris Mill.) belong to the Rosaceae family and are designed as a GRAS ingredient based on the FDA data. Animal experiments and clinical practice confirm that there are many positive pharmacological effects mainly related to anthocyanins. Development of standard regulations for this kind of medicinal plant raw materials is actual to introduce sour cherry fruits into official medical practice. In our research, we investigate macroscopic and microscopic properties and determine the content of tanning substances and anthocyanins in sour cherry fruits of various kinds of conservation. Materials and methods: Modern complex of physicochemical methods was used in pharmacognostical research. Microscopic analysis was performed on a LOMO Micmed-6 microscope with 10× eyepieces, 4×, 10×, 40× and 100× objectives, photographies were taken with a Sony Xperia Z3 compact digital camera. The content of total anthocyanins in terms of cyanidin-3-?-glucoside was determined by differential UV-spectrophotometry, total tannins content – by redox titration. Results and discussion: The anomocytic stomatal complex, fruits pulp druses, anthocyanins containing in the epidermis and fruits pulp cells, and vessels are typical signs for the sour cherry fruits anatomy structure. A conservation method has not to effect on visual representation of diagnostic signs. The contents of tanning substances are little; it is from 2.73±0.08% to 4.12±0.13% in terms of tannin. Maximal anthocyanin contents (in terms of cyanidin-3-glucoside and in absolutely dried raw material) were determined in fresh-frozen sour cherry fruits (after defrosting (with juice and without kernel) is 5.40±0.15% or in terms of moisture-containing raw materials (without kernel) – 0.690±0.019%. In the defrosted fruits without juice, the content of anthocyanins is two times lower in terms of completely dried raw material (without kernel), it is 2.77±0.12% or in terms of moisture-containing raw material (without kernel), it is 0.410±0.018%. In the juice released from cherry fruits after defrosting anthocyanin content in terms of cyanidin-3-glucoside is of 0.450±0.007% (g/100ml juice), in dried sour cherry fruits (without kernel) – 4.34±0.17%, which is lower than in fresh-frozen raw materials. It can be explained by the varietal features, as well by the conservation conditions; this matter is to be further studied. Contents of tanning substances in fresh-frozen and dried sour cherry fruits when storage during 4 months is insignificantly reduced, but it is established that contents of anthocyanin in terms of cyanidin-3-glucoside reduces 1.5 times in 4 months, if the raw material is frozen, and it reduces by 10% if the raw material is dried. Conclusion: Diagnostic signs of macroscopic and microscopic structures, contents of tanning substances and anthocyanins in terms of cyanidin 3-glycoside in sour cherry fruits were studied. The content of the main groups of biologically active substances decreases equally during storage of dried and frozen cherry fruits. When defrosting, it is necessary to carefully collect the juice, as it contains the bulk of the anthocyanins. It is promising to study various options for freezing and drying of cherry fruits with kernels in order to reduce the loss of fruits juice to a minimum. In this case, it is important to ensure that the kernels are whole (not destroyed), in order to avoid the ingress of cyanogenic glycosides into the medicinal plant material. The results will be used to develop normative documentation for sour cherry fruits.


Cite this article:
Kovaleva T.Yu., Bokov D.O., Sergunova E.V., Bobkova N.V., Kovalev I.S., Chernova S.V., Nesterova N.V., Pavlenko A.A., Dorovskikh E.A., Bessonov V.V., Luferov A.N.. Influence of Conservation Methods on the sour cherry (Cerasus vulgaris Mill.) fruits quality. Research Journal of Pharmacy and Technology. 2024; 17(6):2443-1. doi: 10.52711/0974-360X.2024.00382

Cite(Electronic):
Kovaleva T.Yu., Bokov D.O., Sergunova E.V., Bobkova N.V., Kovalev I.S., Chernova S.V., Nesterova N.V., Pavlenko A.A., Dorovskikh E.A., Bessonov V.V., Luferov A.N.. Influence of Conservation Methods on the sour cherry (Cerasus vulgaris Mill.) fruits quality. Research Journal of Pharmacy and Technology. 2024; 17(6):2443-1. doi: 10.52711/0974-360X.2024.00382   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-6-1


REFERENCES:
1.    Shi S, Li J, Sun J, Yu J, Zhou S. Phylogeny and classification of Prunus sensu lato (Rosaceae). Journ. Integrative Plant Biology. 2013. 55(11): 1069-1079.
2.    Ufimov RA. The genus Prunus L. – Plum. Flora of Lower Volga. 2(2). Choripetalous and dicotyledonous flower plants (Crassulaceae - Cornaceae). Publishing editor is N.M. Reshetnikova; the Tsytsin Main Moscow Botanical Garden of Academy of Sciences. Science Edition Society КМК, Moscow, 2018, 117-127. [in Russian].
3.    Tzvelev NN. Manual of the vascular plants of North-West Russia (Leningrad, Pskov and Novgorod provinces). St-Petersburg State Chemical-Pharmaceutical Academy Press, St.-Petersburg, 2000. [in Russian].
4.    Takhtajan AL. Flowering Plants. 2th ed. Springer Science + Business media B.V., Ney York, 2009.
5.    Avdeev VI. Molecular evolution in the subfamily Prunoideae Focke. Bulletin Orenburg State Pedagogical University. Electronic Scientific Journ. (Online) http.//www.vestospu.ru 2012; 2(2): 1-7. [in Russian].
6.    Yandovka LF. Follow-up on the issue of systematic position of cherry and sweet cherry (Rosaceae). Izvestia: Herzen University Journ. Humanities & Sciences. 2015. 173: 125-132. [in Russian].
7.    Spicyn IP. Ecological research of cherry. Tambov University Review. Set: Hard and Technical Sciences. 2002; 7(1): 125-128. [in Russian].
8.    Levgerova NS, Makarkina MA, Jigadlo EN. Varietal features of contents of P-active substances in cherry products. Proceedings of Voronezh State University, set: Geography. Geoecology. 2010; 2: 124-126. [in Russian].
9.    Sinukhova NT, Lunina LV. Biochemical and technological characteristics of sour cherry fruits. New Technologies. 2011; 4: 78-80. [in Russian].
10.    Makarova NV,Voronina MS. Characteristics of chemical composition and antioxidant activity of cherry and cherry products. News of Higher Educational Institutions. Food Technology. 2015; 1(343): 13-15. [in Russian].
11.    Deyneka LA, Chulkov AN, Deyneka VI, Sorokopudov VN, Shevchenko SM. Anthocyanins containing in cherry fruits and in related plants Belgorod State University Scientific Bulletin Set: Natural Science 2011; 15-1(104): 367-373. [in Russian].
12.    Butenko LI, Podgornaya ZhV. Study of the cryogenic treated anthocyanin berry complex. Advances in Current Natural Sciences. 2016; 11-1: 14-17. [in Russian].
13.    Imtiyaz A, Shariq S, Roohi Z. A review on sour cherry (Prunus cerasus): A high value Unani medicinal fruit. International Journal of Green Pharmacy (IJGP). 2017; 11(1): 1-6.
14.    Wojdyło A, Nowicka P, Laskowski P, Oszmiański J. Evaluation of Sour Cherry (Prunus cerasus L.) Fruits for Their Polyphenol Content, Antioxidant Properties, and Nutritional Components. Journal of Agricultural and Food Chemistry, 2014; 62(51): 12332-12345.
15.    Cao J, Jiang Q, Lin J, Li X, Sun C, Chen K. Physicochemical characterisation of four cherry species (Prunus sp.) grown in China. Food Chemistry. 2015; 173: 855-863.  
16.    Prichko TG, Chalaya LD. Biochemical estimate of cherry juice. New Technologies. 2015; 3: 34-39.
17.    Podorovskaya PA, Tarun EI, Selina DS. Antioxidant properties of cherry, raspberry, and strawberry juices. “Sakharov Readings 2018: Environmental Problems of the XXIst Century”. 3 parts of 18th International Scientific Conference papers edited by S.A. Maskevich and S.S. Poznyak. Publishing house: Data-processing center of Ministry of Finance of the Republic of Belarus. 2018; 162-164. [in Russian].
18.    Karanik OS, Krivorot AM. Qualitative indicators of sour cherry fruits after short keeping. /Fruit growing. Collection of tractates. Belarus, 2013 Publishing house: Republic Scientific Industrial Subsidary Commercial Enterprise “Institute for Fruit Growing” (Samokhalovichi). 2013; 25: 489-496. [in Russian].
19.    Borzykh NV, Yuskov AN, Dubrovskaya OYu, Denisova AV. Using of remade cherry fruits. Selection and sorting of garden cultures. Competitive sorts and high quality gardening technologies. Orel, June, 02-05, 2015. Papers of the International Research-to-Practice Conference devoted to 170 anniversary of VNIISPK. Publishing house: Russian Research Zhilin Institute of Fruit Crop Breeding. 2015; 24-25. [in Russian].
20.    Blando F, Oomah BD. Sweet and Sour Cherries: Origin, Distribution, Nutritional Composition and Health Benefits, Trends in Food Science & Technology. 2019. 86: 517-529.
21.    McCune LM, Kubota C, Stendell-Hollis NR, Thomson CA. Cherries and Health: A Review. Critical Reviews in Food Science and Nutrition. 2010; 51(1): 1-12.
22.    Karomatov IJ, Karomatov SI. Medical significance of cherry and sweet cherry (Literature review) // Biology and Integrative Medicine. 2016; 2: 162-178. [in Russian].
23.    Azaryan E, Malekaneh M, Shemshadi NM, Haghighi F. Therapeutic effects of aqueous extracts of Cerasus avium stem on ethylene glycol-induced kidney calculi in rats. Urology Journ. 2017; 14(4): 4024-4029.
24.    Bajerska J, Mildner-Szkudlarz S, Górnaś P, Seglina D. The effects of muffins enriched with sour cherry pomace on acceptability, glycemic response, satiety, and energy intake: a randomized crossover trial. Journ. Sci. Food Agric. 2016; 96(7): 2486-2493.
25.    Bell PG, Stevenson E, Davison GW, Howatson G. The effects of Montmorency tart cherry concentrate supplementation on recovery following prolonged, intermittent exercise. Nutrients. 2016; 8(7): 441.
26.    Bialasiewicz P, Prymont-Przyminska A, Zwolinska A, et al. Sour cherries but not apples added to the regular diet decrease resting and fMLP-stimulated chemiluminescence of fasting whole blood in healthy subjects. Journ. American College Nutrition. 2018; 37(1): 24-33.
27.    Brown MA, Stevenson EJ, Howatson G. Montmorency tart cherry (Prunus cerasus L.) supplementation accelerates recovery from exercise-induced muscle damage in females. European Journ. Sport Sci. 2018; 1-8.
28.    Cásedas G, Les F, Gómez-Serranillos MP, Smith C, López V. Bioactive and functional properties of sour cherry juice (Prunus cerasus). Food Funct. 2016; 7(11): 4675-4682.
29.    Chai SC, Davis K, Wright RS, Kuczmarski MF, Zhang Z. Impact of tart cherry juice on systolic blood pressure and low-density lipoprotein cholesterol in older adults: a randomized controlled trial. Food Funct. 2018; 9(6): 3185-3194.
30.    Desai T, Bottoms L, Roberts M. The effects of Montmorency tart cherry juice supplementation and FATMAX exercise on fat oxidation rates and cardio-metabolic markers in healthy humans. European Journ. Appl. Physiol. 2018; 118(12): 2523-2539.
31.    Gong X, Xiangcheng X. Antidiabetic effect of hydro-methanol extract of Prunus cerasus L. fruits and identification of its bioactive compounds. Tropical Journal of Pharmaceutical Research (Trop. Journ. Pharm. Res.) 2019; 18(3): 597-602.
32.    Homoki J, Gyémánt G, Balogh P, et al. Sour cherry extract inhibits human salivary α-amylase and growth of Streptococcus mutans (a pilot clinical study). Food Funct. 2018; 9(7): 4008-4016.
33.    Keane KM, Bailey SJ, Vanhatalo A, Jones AM, Howatson G. Effects of Montmorency tart cherry (Prunus cerasus L.) consumption on nitric oxide biomarkers and exercise performance. Scand. Journ. Med. Sci. Sports. 2018; 28(7): 1746-1756.
34.    Keane KM, George TW, Constantinou CL, Brown MA, Clifford T, Howatson G. Effects of Montmorency tart cherry (Prunus cerasus L.) consumption on vascular function in men with early hypertension. American Journ. Clinical Nutrition. 2016; 103(6): 1531-1539.
35.    Keane KM, Bell PG, Lodge JK, et al. Phytochemical uptake following human consumption of Montmorency tart cherry (Prunus cerasus L.) and influence of phenolic acids on vascular smooth muscle cells in vitro. European Journ. Nutrition. 2016; 55(4): 1695-1705.
36.    Keane KM, Haskell-Ramsay CF, Veasey RC, Howatson G. Montmorency tart cherries (Prunus cerasus L.) modulate vascular function acutely, in the absence of improvement in cognitive performance. British Journ. Nutrition. 2016; 116(11): 1935-1944.
37.    Kent K, Charlton K, Roodenrys S, et al. Consumption of anthocyanin-rich cherry juice for 12 weeks improves memory and cognition in older adults with mild-to-moderate dementia. European Journ. Nutrition. 2017; 56(1): 333-341.
38.    Le Phuong Nguyen T, Fenyvesi F, Remenyik J, et al. Protective effect of pure sour cherry anthocyanin extract on cytokine-induced inflammatory caco-2 monolayers. Nutrients. 2018; 10(7): 861.
39.    Losso JN, Finley JW, Karki N, et al. Pilot study of the tart cherry juice for the treatment of insomnia and investigation of mechanisms. American Journ. Therapy. 2018; 25(2): e194-e201.
40.    Saleh FA, El-Darra N, Raafat K. Hypoglycemic effects of Prunus cerasus L. pulp and seed extracts on alloxan-induced diabetic mice with histopathological evaluation. Biomed. Pharmacotherapy. 2017; 88: 870-877.
41.    Shilova IV, Suslov NI, Otmakhov VI, Zibareva LN, Samylina IA, Mazin EV, Petrova EV, Babushkina MS, Kovaleva TYu, Kuskova IS, Krapivin AV. Chemical and pharmacological study of herbal preparations that improve cognitive-mnestic functions. Pharmaceutical Chemistry Journal. 2017; 50(10): 654-658.
42.    Vitale KC, Hueglin S, Broad E. Tart cherry juice in athletes: A literature review and commentary. Current Sports Med. Rep. 2017; 16(4): 230-239.
43.    Yılmaz FM, Görgüç A, Karaaslan M, et al. Sour cherry by-products: Compositions, functional properties and recovery potentials – a review. Crit. Rev. Food Sci. Nutr. 2018; 1-15.
44.    Sergunova EV. Preservation methods of medicinal raw materials and modern aspects of using of plant raw materials and natural raw materials in medicine / in the collection: Aspects of the current use of medical plant and natural raw materials. V Research-to-practice conference. Pharmacy and Traditional Medicine Institute and I.M. Sechenov First Moscow State Medical University. Ministry of Public Health of the Russian Federation. 2017; 190-193. [in Russian].
45.    State Pharmacopoeia of the Russian Federation XV edition. 2018. Free access: http://doi.femb.ru/femb/pharmacopea.php. (07.10.2019) [in Russian].
46.    Kowalczyk E, Krzesiński P, Kura M, Szmigiel B, Błaszczyk J. Anthocyanins in medicine. Polish Journ. Pharmacology. 2003; 55: 699-702.
47.    Burton-Freeman B, Sandhu A, Edirisinghe I. Anthocyanins. Nutraceuticals, 2016, pp. 489-500. https:// doi:10.1016/b978-0-12-802147-7.00035-8 ISBN 9780128021477
48.    Sergunova E.V., Sorokina A.A. Prospects for freeze preservation of medicinal plant raw materials. Pharmacy, 2018; 3 (67): 8-13 [in Russian].
49.    Sergunova E.V., Bokov D.O., Bobkova N.V., Kovaleva T.Yu., Chromchenkova E.P., Bessonov V.V. Amino acid profile and content in crude herbal drugs (fruits) of Rosaceae species. Systematic Reviews in Pharmacy. 2020; 11 (5): 322-329.
50.    Sergunova E.V., Bokov D.O. Influence of conservation method (freezing, drying) on composition and content of biologically active substances in Rosaceae fruits (crude herbal drugs). Asian Journal of Pharmaceutical and Clinical Research. 2019; 12(1): 542-547.
51.    Mulik MB, Deshmukh GS, Phale MD. Stability studies of Garcinia indica by determination of anthocyanins. Research J. Pharmacognosy and Phytochemistry. 2010; 2(2): 144-147.
52.    Shukla RK. A review on european plum (Prunus domestica) for its pharmacological activities and phytochemicals. Research J. Pharm. and Tech. 2021; 14(2):1155-1162.
53.    Lenchyk LV, Ovezgeldiyev D, Shapoval OM, Baiurka SV, Ayaou A. Study of chemical composition and diuretic activity of cherry fruit extract. Research J. Pharm. and Tech. 2018; 11(7): 3036-3042.
54.    Lenchyk LV,Saidov NB. Investigation of phenolic compounds in cherry leaves extract. Research J. Pharm. and Tech. 2017; 10(12): 4402-4406.
55.    Bokov DO, Nizamova LA, Morokhina SL et al. Pharmacognostic studies of Origanum L. species Medicinal plant raw materials. Research Journal of Pharmacy and Technology. 2020; 13(9), 4365-4372.
56.    Bokov DO, Kulaeva IR, Potanina OG et al. Carbohydrates determination in the Snowdrops (Galanthus L.) herbal pharmaceutical substances by TLC and UV-Spectrophotometry. Research Journal of Pharmacy and Technology. 2020; 13(1):243-249.
57.    Sindhu RK, Kaur P. Regulatory requirements and stability testing of ethnomedicinal plant products. Research J. Pharm. and Tech. 2020; 13(3):1538-1542.
58.    Gauthami R, Reddy GS, Austin A, Gopal TK, Reddy C. Stability studies of a polyherbal formulation, Urito under accelerated conditions. Research Journal of Pharmacognosy and Phytochemistry. 2011. 3(3): 128-133.
59.    Jaiswal S, Chavhan SA, Shinde SA, Wawge NK. New tools for herbal drug standardization. Asian J. Res. Pharm. Sci., 2018; 8(3): 161-169.
60.    Vatnikov Y, Shabunin S, Karamyan A et al. Antimicrobial activity of Hypericum perforatum L. International Journal of Pharmaceutical Research. 2020; 12: 723-730.
61.    Vatnikov Y, Rudenko P, Shopinskaya M et al. Effectiveness of biologically active substances from Hypericum perforatum L. in the complex treatment of purulent wounds. International Journal of Pharmaceutical Research. 2020; 4(12): 1108-1117.
62.    Zhilkina V, Sachivkina NP, Ibragimova AN, Kovaleva TY, Molchanova MA, Radeva DV. Methods for the identification and quantitative analysis of biologically active substances from vitamin plants raw material. FEBS Open Bio.  2019; 9(S1): 285-286.
63.    Ekka NR, Namdeo KP, Samal PK. Standardization strategies for herbal drugs – an overview. Research J. Pharm. and Tech. 2008; 1(4): 310-312.
64.    Sagar K, Aneesha S, Uppin P. Phytochemical studies and quantification of total content of phenols, tannins and flavonoids in selected endangered plant species. Res. J. Pharmacognosy and Phytochem. 2018; 10(4): 277-281.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available