Author(s): Bharathy K, Bhagyalakshmi B, Punitha D

Email(s): bharathyk30@gmail.com

DOI: 10.52711/0974-360X.2024.00312   

Address: Bharathy K*, Bhagyalakshmi B, Punitha D
PG Scholar, National Institute of Siddha, Tambaram Sanatorium, Chennai 47, Tamil Nadu, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
Background: Siddha medicine is one among the popular traditional systems of medicine in treating various diseases. Vitiligo is a primary autoimmune de-pigmentary disorder. Generally, melanin pigment production is diminished in vitiligo. By improving tyrosinase activity, melanogenesis can be achieved in vitiligo. Hence the phytocomponents which bind with the target Tyrosinase enzyme, act as a potential treatment for vitiligo. Objective: The objective of this study is to find the lead molecules that bind with these core bio active amino acid residues namely His 190, His54, His63, His194, His38 and His216.These bioactive residues mediates the enzymatic action of tyrosinase enzyme and tends to enhance the action of tyrosinase enzyme which improves melanogenesis. Methods: Auto dock program was used for the molecular docking studies against Tyrosinase enzyme. Results: From reported data of the herb, the phytochemicals Myricetin reveals highiest of 4 interactions with the core active amino acid residues of the target Tyrosinase enzyme.Second highiest level is reached by the compounds such as Catechin, Apigenin and Cinnamic acid with the 3 interactions with the active site . Gallic acid and Quercetin reveal 2 interactions over the target enzyme. From the results of docking study of the herb, the leads such as Catechin, Myricetin, Apigenin and Cinnamic acid possess 3-4 interactions with core target amino acids of Tyrosinase enzyme and helps in treatment and management of vitiligo. Conclusion: These phytochemicals exhibit anti-vitiligo activity by harmonising the action of tyrosinase enzyme in vitiligo treatment. Further clinical trials need to be performed for identifying the efficacy and effectiveness of Thespesia populnea in the treatment and management of vitiligo. Siddha, Thespesia populnea bark, vitiligo, Tyrosinase enzyme, docking study, pigmentation disorder.


Cite this article:
Bharathy K, Bhagyalakshmi B, Punitha D. Anti-vitiligo effect of Thespesia populnea L. bark against Tyrosinase enzyme using In-silico model. Research Journal of Pharmacy and Technology. 2024; 17(5):1968-2. doi: 10.52711/0974-360X.2024.00312

Cite(Electronic):
Bharathy K, Bhagyalakshmi B, Punitha D. Anti-vitiligo effect of Thespesia populnea L. bark against Tyrosinase enzyme using In-silico model. Research Journal of Pharmacy and Technology. 2024; 17(5):1968-2. doi: 10.52711/0974-360X.2024.00312   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-8


REFERENCES:
1.    Krishna Das KV. Textbook of Medicine.6th edition.New Delhi. Jaypee Brothers Medical Publishers (P) Ltd. 2017.1534p
2.    Kumar S, Nayak CS, Padhi T, Rao G, Rao A, Sharma VK, Srinivas CR. Epidemiological pattern of psoriasis, vitiligo and atopic dermatitis in India: Hospital-based point prevalence. Indian Dermatology Online Journal. 2014 Nov;5(Suppl 1);S6.
3.    Handa S, Kaur I. Vitiligo: clinical findings in 1436 patients. J Dermatol. 1999 Oct;26(10);653-7
4.    Martis J, Bhat R, Nandakishore B, Shetty JN. A clinical study of vitiligo. Indian J Dermatol Venereol Leprol. 2002 Mar-Apr;68(2);92-3
5.    Rangani J, Kumari A, Patel M, Brahmbhatt H, Parida AK. Phytochemical profiling, polyphenol composition, and antioxidant activity of the leaf extract from the medicinal halophyte Thespesia populnea reveal a potential source of bioactive compounds and nutraceuticals. J Food Biochem. 2019;43(2);e12731
6.    Vaidiya rathinam Murugesa mudhaliyar K.S.Gunapadam-part 1-Mooligai vaguppu.1st edition. Chennai; Indian Medicine Homoeopathy Department; 2013.208, 211-213p
7.    Bikadi, Z., Hazai, E.Application of the PM6 semi-empirical method to modeling proteins enhances docking accuracy of AutoDock. J. Cheminf. 2009; 1;15
8.    T. A. Halgren. Merck molecular force field. I. Basis, form, scope, parametrization, and performance of MMFF94. Journal of Computational Chemistry.1998; 17 (5-6); 490-519
9.    G. M. Morris, D. S. Goodsell, et al. Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function. Journal of Computational Chemistry. 1998; 19(14); 1639-1662
10.    Hwang YS, Oh SW, Park SH, Lee J, Yoo JA, Kwon K, P-ark SJ, Kim J, Yu E, Cho JY, Lee J. Melanogenic Effects of Maclurin Are Mediated through the Activation of cAMP/PKA/CREB and p38 MAPK/CREB Signaling Pathways. Oxid Med Cell Longev. 2019 Dec 22; 2019: 9827519
11.    Zhou X, Liu X, Zhang G, Zhang Q, Chen H, Wang Y, Fang F, Sun J. Knockdown THOC2 suppresses the proliferation and invasion of melanoma. Bioengineered. 2019 Dec;10(1);635-645
12.    D'Mello SA, Finlay GJ, Baguley BC, Askarian-Amiri ME. Signaling Pathways in Melanogenesis. Int J Mol Sci. 2016 Jul 15; 17(7); 1144
13.    Kingo K, Aunin E, Karelson M, Rätsep R, Silm H, Vasar E, Kõks S. Expressional changes in the intracellular melanogenesis pathways and their possible role in the pathogenesis of vitiligo. J Dermatol Sci. 2008 Oct; 52(1); 39-46
14.    Derouazi M, Wang Y, Marlu R, Epaulard O, Mayol JF, Pasqual N, Le Gouellec A, Polack B, Toussaint B. Optimal epitope composition after antigen screening using a live bacterial delivery vector: application to TRP-2.Bioeng Bugs. 2010 Jan-Feb; 1(1); 51-60.
15.    Xu W, Wang X. Detection of melanocyte lineage-specific genes in vitiligo lesions. Exp Ther Med. 2019 Jun; 17(6); 4485-4491
16.    Jin Y, Birlea SA, Fain PR, Gowan K, Riccardi SL, Holland PJ, Mailloux CM, Sufit AJ, Hutton SM, Amadi-Myers A, Bennett DC, Wallace MR, McCormack WT, Kemp EH, Gawkrodger DJ, Weetman AP, Picardo M, Leone G, Taïeb A, Jouary T, Ezzedine K, van Geel N, Lambert J, Overbeck A, Spritz RA. Variant of TYR and autoimmunity susceptibility loci in generalized vitiligo.N Engl J Med. 2010 May 6; 362(18); 1686-97
17.    Awad SS, Moftah NH, Rashed LA, Touni AA, Telep RAA. Evaluation of the effect of narrow band-ultraviolet B on the expression of tyrosinase, TYRP-1, and TYRP-2 mRNA in vitiligo skin and their correlations with clinical improvement: A retrospective study. Dermatol Ther. 2021 Jan; 34(1); e14649
18.    Al Robaee AA, Alzolibani AA, Rasheed Z. Autoimmune response against tyrosinase induces depigmentation in C57BL/6 black mice. Autoimmunity. 2020 Dec; 53(8); 459-466
19.    Nie HQ, Wang P, Zhang XY, Ding C, Liu J, Xu AE.Relationship between autophagy of melanocytes in patients with vitiligo and clinical types. Zhonghua Yi Xue Za Zhi. 2016 Jul 12; 96(26): 2064-9.
20.    R. Vinodini, A. M. Amala Hazel, M. Meenakshi Sundaram, N. J. Muthukumar. Clinical Evaluation of Parangipattai chooranam (internal) and Annabedhi chenduram (external) for Venpulli (Vitiligo) in children. Research J. Pharm. and Tech. 2019; 12(12): 5932-5936
21.    Alokananda Chakraborty, Priyanka Pallapolu, Seelamneni Thulasamma, Lahari Kuna, Shravan Kumar Ghali, Kaiser Jamil, Ghazala Javed, Munawwar Husain Kazmi. Genetic variations in NLRP1 and Glutathione S-Transferase are associated with Generalized Vitiligo of Phlegmatic Clinical Phenotypes in Unani Medicine Philosophy of South Indian Population. Research Journal of Pharmacy and Technology. 2022; 15(5): 2221-7
22.    B. Vinubharathi, V. Mahalakshmi, N. J. Muthukumar, R. Suresh, V. Banumathi. Screening of In vitro Antioxidant Activity of Methanolic extract of Maruthampattai kudineer- A Siddha Polyherbal Preparation. Research J. Pharm. and Tech. 2019; 12(1): 219-222.
23.    A. Rajendra Kumar, N. J. Muthukumar, A. Faridha. Neermulli kudineer – A Classical Polyherbal Decoction in Siddha System of Medicine. Research J. Pharm. and Tech. 2019; 12(1): 445-460.
24.    Ravi Kumar, Sachin Tyagi. A Review on Natural Treatment’s of Vitiligo. Asian J. Pharm. Res. 2020; 10(4):263-267.
25.    P. Prapulla. A Review on: Vitiligo- A Non Contagious Chronic Disease different Types and Treatments. Asian J. Research Chem. 2019; 12(2): 120-125.
26.    R Parthasarathy, R Ilavarasan, CM Karrunakaran. Pharmacognostical Studies on Thespesia populnea Bark. Research J. Pharmacognosy and Phytochemistry. 2009; 1(2): 128-131
27.    R. Parthasarathy, Amrender Singh, Debjit Bhowmik. In vitro Antioxidant Activity of Bark and Leaf of Thespesia populnea.Res. J. Pharmacognosy & Phytochem. 2016; 8(1): 01-04
28.    Shaikh Habeeba, Shaikh Siddiqua. Babchi unani marketed formulation effective in treatment of vitiligo: A case study. Research Journal of Pharmacognosy and Phytochemistry. 2022; 14(1):1-4
29.    Shunmugaram S. Physiochemical and Acute Toxicity Analysis of a Siddha Herbo-Mineral Formulation – Pachai Karpoora Vadagam (PKV). Research J. Pharm. and Tech. 2019; 12(1): 227-230

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available