Author(s): Alapati Sahithi, Choppala Asha Deepti

Email(s): salapati1@gitam.in

DOI: 10.52711/0974-360X.2024.00353   

Address: Alapati Sahithi*, Choppala Asha Deepti
Department of Pharmaceutical Analysis, GITAM School of Pharmacy, Visakhapatnam, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
Trastuzumab and Pembrolizumab, are both integral to cancer treatment. This study focused on the development of a Reverse-phase high-performance liquid chromatography technique that has been validated for simultaneous assay of Trastuzumab and Pembrolizumab using Luna Phenyl Hexyl column with UV detection at 215 nm, and the run time is 5 minutes. The retention times for Trastuzumab 2.730 min and Pembrolizumab 4.468 min were determined. The method exhibited a concentration range of 110–660 µg/ml for Trastuzumab and 12.50–75 µg/ml for Pembrolizumab. The Limit of Detection and Limit of Quantification were determined to be 0.66µg/ml and 2µg/ml for Trastuzumab,0.075 µg/ml and 0.25 µg/ml for Pembrolizumab, respectively.


Cite this article:
Alapati Sahithi, Choppala Asha Deepti. Stability indicating RP-HPLC method for the simultaneous estimation of Trastuzumab and Pembrolizumab in pharmaceutical dosage forms. Research Journal of Pharmacy and Technology. 2024; 17(5):2241-6. doi: 10.52711/0974-360X.2024.00353

Cite(Electronic):
Alapati Sahithi, Choppala Asha Deepti. Stability indicating RP-HPLC method for the simultaneous estimation of Trastuzumab and Pembrolizumab in pharmaceutical dosage forms. Research Journal of Pharmacy and Technology. 2024; 17(5):2241-6. doi: 10.52711/0974-360X.2024.00353   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-49


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