Author(s): Lakade Mayuri M, Kale RN, Dyade GK

Email(s): lakademayuri734@gmail.com

DOI: 10.52711/0974-360X.2024.00351   

Address: Lakade Mayuri M*, Kale RN, Dyade GK
Department of Pharmaceutical Chemistry, SVPM’S College of Pharmacy, Malegaon (BKII), Baramati, Dist. Pune, 413115, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
Objective: A reverse- phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for estimation of combined formulation containing tenofovir(disoproxilfumarate) (TDF) and emtricitabine (EMT). Method: Chromatographic separation was performed on RP-C18 column. The optimized mobile phase of composition (Methanol: Water and pH adjusted to 3.8 with acetic acid was pumped withflow rate of 0.8ml/min in the 70:30% v/v) ratio and the eluents were monitored at 261nm. Results: The assay was performed with tablet and percentage of assay was found to 99.91% for TDF and 99.69% for EMT respectively. Linearity was obtained in the concentration range of 2-12µg/ml for TDF and 2-12µg/ml for EMT. The method was statistically validated and RSD was found to be <2%, indicating high degree of accuracy and precision of the proposed RP-HPLC method. Conclusion: The method suggests usefulness of unique mobile phase during the estimation of combination of dosage forms. Due to its simplicity, rapidness, high precision and accuracy, the proposed RP-HPLC method can be applied for simultaneous determination of TDF and EMT in pharmaceutical dosage form.


Cite this article:
Lakade Mayuri M, Kale RN, Dyade GK. RP-HPLC Method Development and Validation for Simultaneous Estimation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2024; 17(5):2229-4. doi: 10.52711/0974-360X.2024.00351

Cite(Electronic):
Lakade Mayuri M, Kale RN, Dyade GK. RP-HPLC Method Development and Validation for Simultaneous Estimation of Tenofovir Disoproxil Fumarate and Emtricitabine in Pharmaceutical Dosage Form. Research Journal of Pharmacy and Technology. 2024; 17(5):2229-4. doi: 10.52711/0974-360X.2024.00351   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-47


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