Author(s):
Thirumal M, Monika S, Kumar P R, Rohil S S
Email(s):
thirumam@srmist.edu.in
DOI:
10.52711/0974-360X.2024.00308 
Address:
Thirumal M*, Monika S, Kumar P R, Rohil S S
Department of Pharmacognosy, SRM College of Pharmacy, SRMIST, Kattankulathur, Chengalpet district, 603203.
*Corresponding Author
Published In:
Volume - 17,
Issue - 5,
Year - 2024
ABSTRACT:
Aim: The study aims to evaluate the in-vitro anti-cancer effect of the ethanolic extract of Xanthium indicum Koenig (Asteraceae) Materials and Methods: The plant was chosen and extracted by Maceration method. The extraction was processed using the following solvents such as Petroleum ether, Chloroform, ethyl acetate, and ethanol. TLC and HPTLC analysis was performed using the solvents, and the Rf values was noted. The in vitro antioxidant using DPPH scavenging assay and in-vitro anti-cancer activity using MTT assay and Apoptosis by ethanolic extract of Xanthium indicum plant. The IC50 value was noted accordingly. Result: Ethanol was considered as a better solvent; in each test, only ethanol showed comparatively high value than others. MTT assay revealed that the viability of the cell increases with increase in concentration, similarly in Apoptosis method cell death was seen as concentration increases. Conclusion: Ethanolic extract of Xanthium Indicum was found to possess high potentiality. Hence, it can be subjected to study further, and prominent anti-cancer research can be done.
Cite this article:
Thirumal M, Monika S, Kumar P R, Rohil S S. In-vitro Antioxidant and Anticancer activity of Aerial parts of Xanthium indicum koenig (Asteraceae). Research Journal of Pharmacy and Technology. 2024; 17(5):1945-0. doi: 10.52711/0974-360X.2024.00308
Cite(Electronic):
Thirumal M, Monika S, Kumar P R, Rohil S S. In-vitro Antioxidant and Anticancer activity of Aerial parts of Xanthium indicum koenig (Asteraceae). Research Journal of Pharmacy and Technology. 2024; 17(5):1945-0. doi: 10.52711/0974-360X.2024.00308 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-4
REFERENCES:
1. Graciano AJ, Chone CT, Fischer CA, Bublitz GS PA. Repeated fine-needle aspiration cytology for the diagnosis and follow-up of thyroid nodules. Brazilian J Otorhinolaryngol. 2014;80:422–7.
2. Chouni A, Pal A, Gopal PK, Paul S. GC-MS Analysis and Screening of Anti-Proliferative Potential of Methanolic Extract of Garcinia cowa on Different Cancer Cell Lines. Pharmacogn J [Internet]. 2020 Mar 4;13(2):347–61. Available from: http://www.phcogj.com/article/1369
3. Rohil SS, Kumar PR. Xanthium indicum Koenig-A Review. Res J Pharm Technol [Internet]. 2020;13(5):2475. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=13&issue=5&article=072
4. Jayaraman S, Rajagopal P, Veeraraghavan V, Sivagnanam P, Ravikumar D, Suga SSD, et al. Black Horehound (Ballota nigra Linn) Induces Apoptosis in Prostate Cancer Cells (PC-3) Through Intrinsic Signalling Cascade. Pharmacogn J [Internet]. 2020 Sep 15;12(6):1377–82. Available from: https://www.phcogj.com/article/1256
5. Othman SNN, Lum PT, Gan SH, Mani S, Sekar M. Protective Effect of Natural Products against Chemotherapy-Induced Cardiotoxicity: A Review. Pharmacogn J [Internet]. 2020 Aug 5;12(5):1180–9. Available from: http://phcogj.com/article/1211
6. Thite SV, Kore BA, Camacho-Tapia M, Tovar-Pedraza JM. First report of Podosphaera xanthii causing powdery mildew on Xanthium strumarium in India. J Plant Pathol. 2018 Apr;100(1):129–129.
7. Monika S, Thirumal M KPR. Identification Of Bioactive Phytochemicals By GC-MS Profiling, Phytochemical Screening, And TLC Using Methanolic Extract Of Carissa Carandas Linn Leaf. J Pharm Negat Results. 2022;13(8):4439–4444.
8. Singh SA, Gowri K, Chitra V. A Review on Phytochemical Constituents and Pharmacological Activities of the plant: Aerva lanata. Res J Pharm Technol [Internet]. 2020;13(3):1580. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=13&issue=3&article=091
9. Arous B, Al-Mardini MA, Ghazal H, Al-Lahham F. Stability-Indicating Method for the Determination of Rivaroxaban and its Degradation Products using LC-MS and TLC. Res J Pharm Technol [Internet]. 2018;11(1):212. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=11&issue=1&article=040
10. Kharat SN, Ansari N, Mendhulkar VD. HPTLC screening for Flavonoids content in leaf extracts of Syzygium cumini (Linn.) and its Antimicrobial activity. Res J Pharm Technol [Internet]. 2020;13(6):2720. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=13&issue=6&article=034
11. Patel AJ, Joshi DM, Choyal MS, Patel BD. Development and validation of stability indicating HPTLC method for simultaneous estimation of Fluocinolone acetonide and Miconazole nitrate in Ointment. Asian J Pharm Anal [Internet]. 2015;5(2):49. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:ajpa&volume=5&issue=2&article=001
12. Panth N, Paudel KR, Parajuli K. Reactive Oxygen Species: A Key Hallmark of Cardiovascular Disease. Adv Med [Internet]. 2016;2016:1–12. Available from: https://www.hindawi.com/journals/amed/2016/9152732/
13. Salama ZA, Aboul-Enein AM, Gaafar AA, Asker MS, Aly HF, Ahmed HA. In-vitro Antioxidant, Antimicrobial and Anticancer Activities of Banana leaves ( Musa acuminata ) and Olive leaves ( Olea europaea L.) as by-products. Res J Pharm Technol [Internet]. 2020;13(2):687. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=13&issue=2&article=036
14. Kumar S, Nair R, Gupta S, Abdullah A, Talwar P, Ravanan P. Anti-Cancer and Neuro-Protective effect of Cuminum cyminum extracts on IMR32 Human Neuroblastoma Cell Lines. Res J Pharm Technol [Internet]. 2018;11(4):1547. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=11&issue=4&article=052
15. Devi DM, Banu N. Anti-Proliferative activity of Chlorophyllin from Phyllanthus Emblica L. against MCF-7 and Vero Cell line. Res J Pharm Technol [Internet]. 2017;10(2):516. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=10&issue=2&article=030
16. Krishnasamy L, Selvam MM, Ravikrishnan B. Anticancer property of Colchicine isolated from Indigofera aspalathoids. Res J Pharm Technol [Internet]. 2016;9(4):386. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=9&issue=4&article=011
17. Sivakumaran N, Samarakoon SR, Adhikari A, Ediriweera MK, Tennekoon KH, Malavige N, et al. Cytotoxic and Apoptotic Effects of Govaniadine Isolated from Corydalis govaniana Wall. Roots on Human Breast Cancer (MCF-7) Cells. Biomed Res Int [Internet]. 2018 Jul 24;2018:1–11. Available from: https://www.hindawi.com/journals/bmri/2018/3171348/
18. Jawad BO. Apoptosis study on the normal and diabetics type 2 patients. Res J Pharm Technol [Internet]. 2018;11(6):2493. Available from: http://www.indianjournals.com/ijor.aspx?target=ijor:rjpt&volume=11&issue=6&article=061