Author(s):
Candra Irawan, Imas Solihat, Sellafiana Sunu Praptiwi, Miska Mufidah, Elisa Listianti, Bulan Safitri
Email(s):
imaskhairani@gmail.com
DOI:
10.52711/0974-360X.2024.00343 
Address:
Candra Irawan, Imas Solihat*, Sellafiana Sunu Praptiwi, Miska Mufidah, Elisa Listianti, Bulan Safitri
Department of Food Nanotechnology, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 5,
Year - 2024
ABSTRACT:
Temu Hitam (Curcuma aeruginosa Roxb) is recognized as one of the plants that has the potential to be utilized in the practice of traditional medicine. The selection of the extraction method is an essential initial step in the process of chemical component purification. In this particular investigation, the extraction process was carried out utilizing a variety of techniques, including hot maceration, cold maceration, and ultrasound-assisted extraction (UAE), in order to investigate the anti-inflammatory, antioxidant, and cytotoxicity capabilities of the subject material. The percent yield from the UAE method showed the highest amount of 51.94%, and the IC50 values from the cold method, hot method, and UAE using the FRAP method were 4.65, 6.22, and 5.57 ppm, respectively. The extract using the UAE method has higher cytotoxicity to Artemia salina Leach larvae. In addition, UAE showed better anti-inflammatory activity with an IC50 value of 2.96 ppm.
Cite this article:
Candra Irawan, Imas Solihat, Sellafiana Sunu Praptiwi, Miska Mufidah, Elisa Listianti, Bulan Safitri. Effect of Extraction Method on Antioxidant Activity, Anti-inflammation and Cytotoxicity of Curcuma aeruginosa Roxb. Rhizome. Research Journal of Pharmacy and Technology. 2024; 17(5):2180-4. doi: 10.52711/0974-360X.2024.00343
Cite(Electronic):
Candra Irawan, Imas Solihat, Sellafiana Sunu Praptiwi, Miska Mufidah, Elisa Listianti, Bulan Safitri. Effect of Extraction Method on Antioxidant Activity, Anti-inflammation and Cytotoxicity of Curcuma aeruginosa Roxb. Rhizome. Research Journal of Pharmacy and Technology. 2024; 17(5):2180-4. doi: 10.52711/0974-360X.2024.00343 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-39
REFERENCE:
1. Tg Kamazeri, T.S.A., Abd Samah, O., Taher, M., Susanti, D., and Qaralleh, H. Antimicrobial activity and essential oils of Curcuma aeruginosa, Curcuma mangga, and Zingiber cassumunar from Malaysia. Asian Pac. J. Trop. Med. 2012; 5(3): 202–209. https://doi.org/10.1016/S1995-7645(12)60025-X
2. Charde, R.M., Charde, M.S., Fulzele, S.V., Satturwar, P.M., Kasture, A.V., Joshi, S.B. Evaluation of ethanolic extract of moringa oleifera for wound healing, anti-inflammatory and antioxidant activities on rats. Research J.Pharm and Tech. 2011; 4(2): 254-258.
3. Srivilai, J., Khorana, N., Waranuch, N., and Ingkaninan, K. Anti-androgenic activity of furanodiene isolated from Curcuma aeruginosa Roxb. Extract. Naresuan Univ. J, Special Issue. 2011; 33– 37.
4. Thomas, Td. dan Jose, S; Comparative phytochemical and anti-bacterial studies of two indigenous medicinal plants Curcuma caesia Roxb. and Curcuma aeruginosa Roxb, International Journal of Green Pharmacy, 2014.
5. George, M., and Britto, S.J. Phytochemical and antioxidant studies on the essential oil of the rhizome of Curcuma aeruginosa Roxb. Int. Res. J. Pharm. 2015; 6(8): 573–579. https://doi.org/10.7897/2230-8407.068113
6. Suphrom, N., Pumthong, G., Khorana, N., Waranuch, N., Limpeanchob, N., and Ingkaninan, K. Antiandrogenic effect of sesquiterpenes isolated from the rhizomes of Curcuma aeruginosa Roxb. Fitoterapia. 2012; 83(5): 864–871. doi: 10.1016/j.fitote.2012.03.017.
7. Hossain, C.F., Al-Amin, M., Md. Sayem, A.S., Siragee, I.H., Tunan, A.M., Hassan, F., Kabir, M.M., and Sultana, G.N.N. Antinociceptive principle from Curcuma aeruginosa. BMC Complementary Altern. Med. 2015; 15(1); 191–198. doi: 10.1186/s12906-015-0720-6
8. Dessy, VJ, Sivakumar, S.R., George, M., and Francis, S. GC-MS analysis of bioactive compounds present in different extracts of rhizome of Curcuma aeruginosa Roxb. J. Drug Delivery Ther. 2019; 9(2-s): 13–19. DOI https://doi.org/10.22270/jddt.v9i2-s.2589
9. Neamsuvan, O., Tuwaemaengae, T., Bensulong, F., Asae, A., and Mosamae, K. A survey of folk remedies for gastrointestinal tract diseases from Thailand’s three southern border provinces. J. Ethnopharmacol. 2012; 144(1): 11–21. doi: 10.1016/j.jep.2012.07.043.
10. Baharun, K., Rukmi, I., Lunggani, A.T., dan Fachriyah, E. Daya Antibakteri Berbagai Konsentrasi Rimpang Temu Hitam (Curcuma aeruginosa roxb.) terhadap Bacillus subtilis dan Staphyloccus aureus Secara Invitro. Jurnal Biologi. 2013; 2(4): 16-24.
11. Sari, A.P., and Supratman, U. Phytochemistry and Biological Activities of Curcuma aeruginosa Roxb.)-Mini review, Indones. J. Chem. 2022; 22(2): 576-598. DOI: 10.22146/ijc.70101
12. Uttara, J., Vijaya, M., Joshi, S.V. Comparative study of conventional and microwave assisted some indigenous drugs. Research J and Pharm and Tech. 2009; 2(2): 417-418.
13. Anatolyevna, S.A., Alexandrovich, K.V., Nokilayevich, S.A., Victorovna, R.M., Nikolaevna, S.K.. Method of obtaining of extract by the method of modified maceration. Reasearch J.Pharm and Tech. 2019; 12(12): 5956-5958.
14. Zam, W., Ali, A., Husein, F. Extraction of polyphenol from oregano and thyme bu maceration using glycerine. Research J.Pharm and Tech. 2020; 13(6): 2699-2702.
15. Aziza NAA, Hashama R, Sarmidi MR, Suhaimi SH, Idris MKH. A review on extraction techniques and therapeutic value of polar bioactives from Asian medicinal herbs: Case study on Orthosiphon aristatus, Eurycoma longifolia and Andrographis paniculate. Saudi Pharmaceutical Journal. 2021; 29(2): 143-165. doi: 10.1016/j.jsps.2020.12.016.
16. Priyanka, S., Kirubagaran, R., Leema, J.T.M. Optimization of ultrasound-assisted extraction (UAE) of zeaxanthin from marine microalgae Dunaliella tertiolecta (NIOT 141) using response surface methodology. Research J.Pharm and Tech. 2021; 14(3): 1729-1735.
17. Deo, S., Janghel, A., Raut, P., Bhosle, D., Verma, C., Kumar, S.S., Agrawal, M., Amit, N., Sharma, M., Giri, T., Tripathi, K., Ajazuddin., Alexander, A. Emerging microwave assisted extraction (MAE) techniques as an innovative green technologies for the effective extraction of the active phytopharmaceuticals. Research J,Pharm and Tech. 2015; 8(5): 655-666.
18. Tao,Y., Wu,D., Zhang, Q.A., and Sun, D.W. Ultrasound-assisted extraction of phenolics from wine lees:Modeling,optimization and stability of extracts during storage. Ultrasonic Sonochemistry, 2014; 21(2): 706-715. doi: 10.1016/j.ultsonch.2013.09.005.
19. Luna SRLD, Ramírez-Garza RE, Saldívar SOS. Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties. The Scientific World Journal. 2020; 1: 38. https://doi.org/10.1155/2020/6792069
20. Dias ALB, Aguiara AC, Rostagno MA. Extraction of natural products using supercritical fluids and pressurized liquids assisted by ultrasound: Current status and trends. Ultrasonics Sonochemistry. 2021; 74(1): 20. https://doi.org/10.1016/j.ultsonch.2021.105584
21. Spigno, G., Tramelli, L. dan De Faveri, D.M. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering. 2010; 81: 200-208. http://dx.doi.org/10.1016/j.jfoodeng.2006.10.021
22. Sukiman, M., Irawan, C., Ismail., Putri, I.D., Utami, A., Dewanta, A., Septian, W.A. Optimization of ultrasound-assisted extraction of Tinospora crispa stem, phytochemical screening, total phenolic content and antigout potential activity. Pharmacogn J. 2022; 14(2): 308-314. DOI:10.5530/pj.2022.14.39
23. Irawan, C., Elya, B., Hanafi, M., Saputri, F.C. Phytochemical screening,antioxidant activity, and anti-inflammatory potential of Rhinachantus nasutus (L.) kurz flower ethano extract. Pharmacogn J, 14, 5, 521-526. 2022. DOI: 10.5530/pj.2022.14.129
24. Parra, A.L., Yhebra, R.S., Sardiñas, I.G., Buela, L.I. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine. 2001; 8: 395–400. http://dx.doi.org/10.1078/0944-7113-00044
25. Momin, M.A., Rashid, Md.M., Urmi, K.F., Rana, Md. S. Phytochemical screening and investigation of antioxidant and cytoxicity potential of different extracts of selected medicinal plants of Bangladesh. Research J. Pharm and Tech. 2013; 6(9): 1042-1050.
26. Zohmachhuana, A., Malsawmdawngliana., Lalnunmawia, F., Mathipi, V., Lalrinzuali, K., Kumar, N.S. Curcuma aeruginosa Robx. Exhibits cytotoxicity in A-549 and HeLa cells by inducing apoptosis through caspase-dependent pathways. Biomedicine and Pharmacotherapy. 2022; 150: 113039.
27. Narayanankutty, A., Sasidharan, A., Job, J.T., Rajagopal, R., Alfarhan, A., Kim, Y.O., Kim, H.J. Mango ginger (Curcuma amada Robx.) rhizome essential oils as source of environmental friendly biocides:comparison of the chemical composition, antibacterial, insecticidal and larvicidal properties of essential oils extracted by different methods. Environmental Research.2021; 202: 111718.
28. Castro MDL, Garcia JLL. Ultrasonic-assisted soxhlet extraction: an expeditive approach for solid sample treatment, application to the extraction of total fat from oleaginous seeds. J Chromatogr A. 2004; 1034: 237-242. doi: 10.1016/j.chroma.2004.02.020.
29. Molyneux, P. The Use of The Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 2004; 26(2): 211-21
30. Mamatha, A. Brine Shrime Lethality Test of Andrographis paniculata. Research J. Pharm and Tech. 2014; 7(7): 743-745.
31. Gouveia, W., Jorge, T.F., Martins, S., Meireles, M., Carolino, M., Cruz, C., Araújo, M.E.M. Toxicity of ionic liquids prepared from biomaterials. Chemosphere. 2014; 104: 51–56. doi: 10.1016/j.chemosphere.2013.10.055
32. Bezzera, J.W.A., Costa, A.R., da Silva, M.A.P., Rocha, M.I., Boligon, A.A., da Rocha, J.B.T., Barros, L.M., Kamdem, J.P. Chemical composition and toxicological evaluation of Hyptis suaveolens (L.) Poiteau (Lamiaceace) in Drosophila melanogaster and Artemia salina. South African Journal of Botany. 2017; 113: 437-442. https://doi.org/10.1016/j.sajb.2017.10.003
33. Benelli, G., Flamini, G., Canale, A., Cioni, P.L., Conti, B. Toxicity of some essential oil formulations against the Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera Tephritidae). Crop Protection. 2012; 42: 223–229. DOI:10.1016/j.cropro.2012.05.024
34. Di Pasqua, R., Hoskins, N., Betts, G., Mauriello, G. Changes in membrane fatty acids composition of microbial cells induced by addiction of thymol, carvacrol, limonene, cinnamaldehyde, and eugenol in the growing media. Journal of Agricultural and Food Chemistry. 2006; 54: 2745–2749. doi: 10.1021/jf052722l.
35. Shrikhande, P.V. Formulation and evaluation of polyherbal topical anti-inflammatory ekulgel. Research J. Pharm and Tech. 2013; 6(1): 118-122.
36. Golwala, D.K., Patel, L.D., Bothara, S.B., Vaidya, S.K., Sahu, A.R., Kumar, S. Anti-inflammatory activity of abutilon indicum Linn. Leaf. Reasearch J.Pharm and Tech. 2010; 3(1): 183-186
37. Wirastuti, M.D., Falah. S., Syaefudin. Inhibitory activity and kinetics of α-Glukosidase by Toona sinensis steam bark extracts. Curr. Biochem. 2022; 9(1): 16-25. DOI: https://doi.org/10.29244/cb.9.1.2
38. Oanh, P.T., Thanh, N.T., Xuyen, D.T., Huong, L.T., Avoseh, O.N., and Ogunwande, I.A. The rhizome essential oil of Curcuma aeruginosa Roxb. (Zingiberaceae) from Vietnam. Trends Phytochem Res. 2018; 2(3): 179–184.
39. Negi JS, Bisht VK, Bhandari AK, Bharti MK, Sundriyal RC.Chemical and pharmacological aspects of Toona (Meliaceae). Res J Phytochem. 2011; 5(1): 14-21. https://scialert.net/abstract/?doi=rjphyto.2011.14.21
40. Ko, H.H., Hung, C.F., Wang, J.P., and Lin, C.N. Antiinflamatory Triterpenoids and Steroids from Ganoderma lucidum and Ganoderma tsugae, Elsevier, pp: 1-6. 2007. DOI: 10.1016/j.phytochem.2007.06.008