Author(s): Chhayaben S. Kagarana, Kunal N. Patel, Advaita B. Patel

Email(s): chhayakagarana@gmail.com

DOI: 10.52711/0974-360X.2024.00320   

Address: Chhayaben S. Kagarana1*, Kunal N. Patel2, Advaita B. Patel3
1Research Scholar, Gujarat Technological University, Gujarat, India.
2Professor, K.B. Raval College of Pharmacy, Gandhinagar, Gujarat, India.
3Professor, Silver Oak College, Ahmedabad, Gujarat, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
A simple efficient stability-indicating validated RP-HPLC method has been developed for the simultaneous determination of Metformin hydrochloride (MET) and Remogliflozin etabonate (REM) in bulk and was applied to marketed formulations. The mobile phase used for detection was Phosphate Buffer (pH 4.0): Acetonitrile (60:40%v/v). Drug peaks were detected by a UV detector at 226nm. The linearity of the developed method was found to be 20-60µg/mL for Metformin hydrochloride and 4-12µg/mL for Remogliflozin etabonate. The Limit of Detection (LOD.) and Limit of Quantitation (LOQ.) were found to be 0.085µg/mL and 0.258µg/mL for MET and 0.010µg/mL and 0.030µg/mL for REM respectively. Hydrolysis by HCl, NaOH, hydrogen peroxide, UV light and temperature were performed on a formulation which proves that the proposed method was specific and will be suitable for routine quality control analysis.


Cite this article:
Chhayaben S. Kagarana, Kunal N. Patel, Advaita B. Patel. Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Metformin Hydrochloride and Remogliflozin Etabonate in Bulk and Tablet Formulation. Research Journal of Pharmacy and Technology. 2024; 17(5):2025-0. doi: 10.52711/0974-360X.2024.00320

Cite(Electronic):
Chhayaben S. Kagarana, Kunal N. Patel, Advaita B. Patel. Stability Indicating RP-HPLC Method Development and Validation for Simultaneous Estimation of Metformin Hydrochloride and Remogliflozin Etabonate in Bulk and Tablet Formulation. Research Journal of Pharmacy and Technology. 2024; 17(5):2025-0. doi: 10.52711/0974-360X.2024.00320   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-16


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