Author(s): Achmad Makin Amin, Arif Nur Muhammad Ansori, Viol Dhea Kharisma, Days Chelsealani Kaaro, Muhammad Hermawan Widyananda, Arli Aditya Parikesit, Joko Pebrianto Trinugroho, Dora Dayu Rahma Turista, Imam Rosadi, Sergazy Duyssembaev, Maksim Rebezov, Pavel Burkov, Pavel Scherbakov, Vikash Jakhmola, Rahadian Zainul

Email(s): rahadianzmsiphd@fmipa.unp.ac.id

DOI: 10.52711/0974-360X.2024.00317   

Address: Achmad Makin Amin1, Arif Nur Muhammad Ansori2, Viol Dhea Kharisma3, Days Chelsealani Kaaro4, Muhammad Hermawan Widyananda3,5, Arli Aditya Parikesit6, Joko Pebrianto Trinugroho7, Dora Dayu Rahma Turista8, Imam Rosadi9, Sergazy Duyssembaev10, Maksim Rebezov11,12, Pavel Burkov13, Pavel Scherbakov14, Vikash Jakhmola15, Rahadian Zainul16*
1Master Program of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia.
2Postgraduate School, Universitas Airlangga, Surabaya, Indonesia.
3Division of Molecular Biology and Genetics, Generasi Biologi Indonesia Foundation, Gresik, Indonesia.
4Bachelor Program of Biotechnology, Technical University of Berlin, Berlin, Germany.
5Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
6Department of Bioinformatics, School of Life Sciences, Indonesia International Institute for Life Sciences, Jakarta, Indonesia.
7Bioinformatics and Data Science Research Center, Bina Nusa

Published In:   Volume - 17,      Issue - 5,     Year - 2024


ABSTRACT:
T cell immunity, like responses of CD4+and CD8+ T-cell, plays an important role to fight against viral infections and pathological harm. Several previous studies have shown the results that rabies virus (RABV) protein can act as an ideal receptor for rabies neuroseptic vaccine by inducing a response of T-cell. In this research, we evaluated possible vaccine epitopes based on the Rabies virus sequence and human lymphocyte antigen (HLA) distribution. First, this study used the rabies virus protein P sequence obtained from the NCBI database. Next, we predicted rabies CTL protein epitopes based on the frequency of HLA-I distribution allele locus A, B, and C in Southeast Asia region (> 1%) using Immune Epitope Database and Analysis Resource (iedb.org). Our results predict the presence of 12 epitopes of the protein P RABV. A cluster analysis of epitopes shows that seven P-protein clusters cover 97.47% of the Southeast Asian population. After a conservative epitope analysis, 8 epitopes of protein P showed protection in 11 different types of isolated Rabies virus. Finally, 4 epitopes (SQTVEEIISY119-128; RSSGIFLYNF218-227, ASGPPALEW178-186, IISYVTVNF125-133) were used to vaccinate 97.47% of people in Southeast Asia. Our results suggest that both single and combined CTL epitopes which were predicted in this study can be used as a more effective alternative vaccine against rabies virus’ infections and development


Cite this article:
Achmad Makin Amin, Arif Nur Muhammad Ansori, Viol Dhea Kharisma, Days Chelsealani Kaaro, Muhammad Hermawan Widyananda, Arli Aditya Parikesit, Joko Pebrianto Trinugroho, Dora Dayu Rahma Turista, Imam Rosadi, Sergazy Duyssembaev, Maksim Rebezov, Pavel Burkov, Pavel Scherbakov, Vikash Jakhmola, Rahadian Zainul. T-Cell Epitope Vaccine Prediction Analysis Targeting Phosphoprotein (P) Rabies Virus Based on the Presence of HLA-I Alleles A, B, and C Loci Throughout Southeast Asia: An Immunoinformatics Study. Research Journal of Pharmacy and Technology. 2024; 17(5):2001-2008 doi: 10.52711/0974-360X.2024.00317

Cite(Electronic):
Achmad Makin Amin, Arif Nur Muhammad Ansori, Viol Dhea Kharisma, Days Chelsealani Kaaro, Muhammad Hermawan Widyananda, Arli Aditya Parikesit, Joko Pebrianto Trinugroho, Dora Dayu Rahma Turista, Imam Rosadi, Sergazy Duyssembaev, Maksim Rebezov, Pavel Burkov, Pavel Scherbakov, Vikash Jakhmola, Rahadian Zainul. T-Cell Epitope Vaccine Prediction Analysis Targeting Phosphoprotein (P) Rabies Virus Based on the Presence of HLA-I Alleles A, B, and C Loci Throughout Southeast Asia: An Immunoinformatics Study. Research Journal of Pharmacy and Technology. 2024; 17(5):2001-2008 doi: 10.52711/0974-360X.2024.00317   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-5-13


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