Author(s): Boulaares Islam, Derouiche Samir, Guemari Imane Yousra


DOI: 10.52711/0974-360X.2024.00292   

Address: Boulaares Islam1,2, Derouiche Samir1,2, Guemari Imane Yousra1
1Department of Cellular and Molecular Biology, Faculty of the Sciences of Nature and Life, El-Oued University, El-Oued 39000, El Oued, Algeria.
2Laboratory of Biodiversity and application of biotechnology in the agricultural field, Faculty of the Sciences of Nature and Life, University of El Oued, El-Oued 39000, Algeria.
*Corresponding Author

Published In:   Volume - 17,      Issue - 4,     Year - 2024

This investigation was aimed to study the effect of leaves aqueous extract of Ocimum basilicum L. (ObE) for prevention of cardiotoxicity and heart failure induced by doxorubicin in rats. For in-vivo rats study, 15 female albino Wistar rats were divided into 3 groups (n = 5); control group, doxorubicin treated rats group (DOX) and rats co-treated with doxorubicin and aqueous extract of O. basilicum group (DOX + O. basilicum. Various hematological, enzymatic, and oxidative stress markers were estimated. Histopathology of heart tissues was observed. Results of in-vivo rats study show that doxorubicin treated rats induced elevation in doxorubicin level in heart tissue, alteration in hematological parameters and enzymatic activity compared to control group. In addition, doxorubicin treated rats induced oxidative stress and histological alteration in heart cells compared to control rats. Co-treatment of doxorubicin with leaves aqueous extract of O. basilicum were partially reversed all of previous parameters. This study indicated that the anti-inflammatory and antioxidant property of leaves aqueous extract of O. basilicum allowed using them to protect organs from the side effects of doxorubicin or from the destructive effects of various diseases.

Cite this article:
Boulaares Islam, Derouiche Samir, Guemari Imane Yousra. Protective effect of ObE against Doxorubicin-Induced immunosuppression and Cardiotoxicity in Rats. Research Journal of Pharmacy and Technology.2024; 17(4):1839-3. doi: 10.52711/0974-360X.2024.00292

Boulaares Islam, Derouiche Samir, Guemari Imane Yousra. Protective effect of ObE against Doxorubicin-Induced immunosuppression and Cardiotoxicity in Rats. Research Journal of Pharmacy and Technology.2024; 17(4):1839-3. doi: 10.52711/0974-360X.2024.00292   Available on:

1.    Erica AG, Paul S, Tim NB, Eward MS, David JH, Ernest H, Nancy H. Molecular mechanisms of the preventable causes of cancer in the United States. Genes and Dev. 2018; 32: 868-902.
2.    Siegel RL, Miller KD, Ahmedin J. Cancer Statistics. CA Cancer J Clin. 2020; 70: 7-30. doi: 10.3322/caac.21590
3.    Lódi M, Priksz D, Fülöp GÁ, Bódi B, Gyöngyösi A, Nagy L … Czuriga D. Advantages of prophylactic versus conventionally scheduled heart failure therapy in an experimental model of doxorubicin-induced cardiomyopathy. J Transl Med. 2019; 17(1): 1-16. doi:10.1186/s12967-019-1978-0
4.    Ibrahim YS, Akram A, Rajab WJ, Ahmed MM, Mahmood MA. Assessment of some Biochemical Parameters among Pleural Liquid and Serum during Platinum-based Chemotherapy against Ovarian Cancer. Research Journal of Pharmacy and Technology. 2022; 15(12):5665-1
5.    Choi WG, Kyun Kim D, Shin Y, Park R, Cho YY, Lee JY ... Lee HS. Liquid Chromatography–Tandem Mass Spectrometry for the Simultaneous Determination of Doxorubicin and its Metabolites Doxorubicinol, Doxorubicinone, Doxorubicinolone, and 7-Deoxydoxorubicinone in Mouse Plasma. Molecules. 2020; 25(5): 1254–. doi:10.3390/molecules25051254
6.    Lei J, Wang H, Zhu D, Wan Y, Yin L. Combined effects of avasimibe immunotherapy, doxorubicin chemotherapy, and metal–organic frameworks nanoparticles on breast cancer. J Cell Physiol. 2019; 1-10.
7.    Aruna P, Gayathiri NM. Cardioprotective Activity of Telmisartan, Metformin and its Combination against Doxorubicin Induced Myocardial Infarction in Rat Model. Research J. Pharm. and Tech. 2018; 11(12): 5293-5296
8.    Adamcova M, Skarkova V, Seifertova J, Rudolf E. Cardiac Troponins are Among Targets of Doxorubicin-Induced Cardiotoxicity in hiPCS-CMs. Int J Mol Sci. 2019; 20 (2638): 1-13. doi:10.3390/ijms20112638
9.    Boulaares I, Derouiche S. Effect of Garlic and Onions Extract Enriched with Honey Treatment on the Lipid Profile, Biochemical and Hematological Biomarker Status in Healthy Women. Pharmaceutical and Biosciences Journal. 2021; 9(1):22-27.
10.    Pandey AK, Tiwari SP, Biswas D, Patel Y, Jajda HM, Dave GS. Evaluation of Phytochemicals, Antioxidant and Anti-inflammatory properties of leaves of Ocimum basilicum L.. Research Journal of Pharmacy and Technology. 2023; 16(4):1981-6.
11.    Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 1977; 28: 49-55.
12.    Ahn MR, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T, 2007, Antioxidant activity and constituents of propolis collected in various area of China, Food Chemistry. 101, 1383-1392.
13.    Southon S, Gee JM, Johnson IT. Hexose transport and mucosal morphology in the small intestine of the zinc-deficient rat. Br J Nutr. 1984; 52(02): 371-380. doi:10.1079/bjn19840103
14.    Al Qahtani AA, Osman A-MM, Damanhouri ZA, Al-Kreathy HM, Al-Malky HS, Ramadan WS, Kamel FO. Cardioprotective Effect of Marine Astaxanthin on Doxorubicin-Induced Cardiotoxicity in Normal Rats. J Pharm Res Int. 2019; 27(3): 1-11. DOI: 10.9734/JPRI/2019/v27i330170
15.    Sastre J, Pallardó FV, García de la Asunción J, Viña J. Mitochondria, oxidative stress and aging. Free Radic Res. 2000; 32(3): 189-198. doi:10.1080/10715760000300201
16.    Weckbecker G, Cory JG. Ribonucleotide reductase activity and growth of glutathione-depleted mouse leukemia L1210 cells in vitro. Cancer Lett. 1988; 40(3): 257-264. Doi : 10.1016/0304-3835(88)90084-5.
17.    Beauchamp C, Fridovich I. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1): 276-287.
18.    Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249: 7130-7139.
19.    Nadeem HR, Akhtar S, Sestili P, Ismail T, Neugart S, Qamar M, Esatbeyoglu T. Toxicity, Antioxidant Activity, and Phytochemicals of Basil (Ocimum basilicum L.) Leaves Cultivated in Southern Punjab, Pakistan. Foods. 2022; 11(9): 1239–. doi: 10.3390/foods11091239.
20.    Atoussi O, Chetehouna S, Derouiche S. Biological properties and Acute Toxicity Study of Copper oxide nanoparticles prepared by aqueous leaves extract of Portulaca oleracea (L). Asian J Pharm Res. 2020; 10(2):89-94.
21.    Boulaares I, Guemari IY, Derouiche S. Analysis of Some Hematological and Biochemical Markers in Women Cancer Patients Receiving Doxorubicine Chemotherapy. Pharmaceutical and Biosciences Journal. 2020; 8(1), 29-34.
22.    Shaty MH, Al-Ezzi MI., Arif IS, Basil D. Effect of Metformin on inflammatory markers involved in Cardiotoxicity induced by Doxorubicin. Research J. Pharm. and Tech. 2019; 12(12): 5815-5821.
23.    Gasser A, Chen Y-W, Audebrand A, Daglayan A, Charavin M, Escoubet B … Nebigil CG. Prokineticin Receptor-1 Signaling Inhibits Dose- and Time-Dependent Anthracycline-Induced Cardiovascular Toxicity Via Myocardial and Vascular Protection. JACC: Cardio Oncology. 2019; 1: 84-102. doi:10.1016/j.jaccao.2019.06.003
24.    Hajare Ashok A, Powar Trupti A, Bhatia Neela M., More Harinath N. Development and Validation of RP-HPLC Method for Determination of Doxorubicin Hydrochloride from Vacuum Foam Dried Formulation. Research J. Pharm. and Tech. 2016; 9(9):1352-1356.
25.    Derouiche S, Zeghib K, Gherbi S, Khelef Y. Protective effects of Aristolochia longa and Aquilaria malaccensis against lead-induced oxidative stress in rat cerebrum. Asian J. Res. Pharm. Sci. 2019; 9(1):57-63.
26.    Sadzuka Y, Sugiyama T, Shimoi K, Kinae N, Hirota S. Protective effect of flavonoids on doxorubicin-induced cardiotoxicity. Toxicol Lett. 1997; 92: 1-7
27.    Hozayen WG, Abou Seif HS. Protective Effects of Rutin and Hesperidin against Doxorubicin-Induced Lipodystrophy and Cardiotoxicity in Albino Rats. Am J Sci. 2011; 7(12): 765-775.
28.    Derouiche S. Current Review on Herbal Pharmaceutical improve immune responses against COVID-19 infection. Research Journal of Pharmaceutical Dosage Forms and Technology. 2020; 12(3): 181-184.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

56th percentile
Powered by  Scopus

SCImago Journal & Country Rank

Recent Articles


Not Available