Author(s): Megawati Megawati, Teni Ernawati, Marissa Angelina, Lia Meilawati, Lucia Dwi Antika, Edi Supriadi

Email(s): twitter @brin_indonesia , megarafandi@gmail.com , teni.ernawati001@gmail.com , marissarfat@gmail.com , liamei83@gmail.com , lucia.dwi.antika@brin.go.id , Edisupriadi127@gmail.com

DOI: 10.52711/0974-360X.2024.00253   

Address: Megawati Megawati1, Teni Ernawati1, Marissa Angelina1, Lia Meilawati1, Lucia Dwi Antika1, Edi Supriadi2
1Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency of Indonesia (BRIN), KST BJ Habibie, Puspiptek, South Tangerang, Indonesia
2Research Center for Chemistry,National Research and Innovation Agency of Indonesia (BRIN), KST BJ Habibie, Puspiptek, South Tangerang, Indonesia
*Corresponding Author

Published In:   Volume - 17,      Issue - 4,     Year - 2024


ABSTRACT:
Free radicals play a significant role in numerous cellular functions, such as cell signaling, metabolism, and defense mechanism. Cassia alata, well known as Yellow Candle bush or locally known as Ketepeng Badak, has been known to possess biological activities including antiinflamation and antidiabetic. This current study investigates the antioxidant potential of C. alata leaves crude extract and resultant fractions. DPPH, ABTS radical scavenging activity, and FRAP assay were evaluated for the determination of the antioxidant activity, while the active constituents in C. alata leaf extract and fractions were identified with LC-QTOF-MS/MS. Total phenolic, flavonoid, and DPPH radical scavenging activities were highest in the ethyl acetate fraction with IC50: 18.54±0.01µg/mL on DPPH assay. Meanwhile, butanol fraction exhibited the strongest activity in ABTS and FRAP tests with IC50 of 4.29±0.26µg/mL and 4.39±0.11µg/mL, respectively. An in silico study using a molecular docking technique was conducted to determine the free energy of binding between human heme oxygenase-1 (HO-1) with bioactive compounds contained in C. alataethanolic leaves extract. Molecular docking of the main constituents in C. alata ethyl acetate fraction showed a greater binding affinity for kaempferol-3-O-ß-D-glucopyranoside (-8.95kcal/mol), followed by coclaurine (-7.94kcal/mol), quercetin (-7.66kcal/mol) and 3-Hydroxy-7-methoxy baicalein (-7.32kcal/mol). These results corroborate the potent antioxidant activity of C. alata extract and fractions and its use as possible antioxidant agents in the pharmaceutical industry.


Cite this article:
Megawati Megawati, Teni Ernawati, Marissa Angelina, Lia Meilawati, Lucia Dwi Antika, Edi Supriadi. Antioxidant activity of Yellow Candle bush (Cassia alata) leaves extract and Bioactive fractions through LC-QTOF-MS/MS and Molecular Docking Approach. Research Journal of Pharmacy and Technology.2024; 17(4):1599-5. doi: 10.52711/0974-360X.2024.00253

Cite(Electronic):
Megawati Megawati, Teni Ernawati, Marissa Angelina, Lia Meilawati, Lucia Dwi Antika, Edi Supriadi. Antioxidant activity of Yellow Candle bush (Cassia alata) leaves extract and Bioactive fractions through LC-QTOF-MS/MS and Molecular Docking Approach. Research Journal of Pharmacy and Technology.2024; 17(4):1599-5. doi: 10.52711/0974-360X.2024.00253   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-4-27


REFERENCES:
1.    Lobo V,  Patil A, Phatak A, Chandra N. Free Radicals, Antioxidants and Functional Foods: Impact on Human Health. J.Pharmacogn. Rev. 2010; 4, 118-126.
2.    Pizzimenti S,Toaldo C, Pettazzoni P, Dianzani MU, Barrera G. The “two-faced” Effects of Reactive Oxygen Species and The Lipid Peroxidation Product 4-hydroxynonenal in The Hallmarks of Cancer.Cancers. 2010; 2: 338-363.
3.    Asmat U, Abad K, Ismail K. Diabetes Mellitus and Oxidative Stress-A Concise Review. J. Saudi.Pharm.  2016; 24: 547-553.
4.    Deng L, DuC, Song P. The Role of Oxidative Stress and Antioxidants in Diabetic Wound Healing. Oxid. Med. Cell Longev. 2021; 1:1-11.
5.    Ghanshyam B, Jadhav, Ravindranath B. Saudagar. Free radical Scavenging and Antioxidant Activity of Punicagranatum Linn. Asian J. Res. Pharm. Sci. 2014; 2:51-54.
6.    Leong LP, Shui G. An investigation of Antioxidant Capacity of Fruits in Singapore markets. J. Food. Chem. 2002; 76: 69-75.
7.    Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant Activities of Peel, Pulp and Seed Fractions of Common Fruits as Determined by FRAP Assay. J. Nutr. Res. 2003; 23: 1719-1726.
8.    Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant Activity Applying an Improved ABTS Radical Cation Decolorization Assay. J. Free. Radic. Biol. Med. 1999; 26: 1231-1237.
9.    Gulcin I. Antioxidants and antioxidant methods-An updated overview. Archives of Toxicology 2020; 94: 651-715.
10.    Kondragunta K, Karuppuraj V, Perumal K. Antioxidant activity and Folic acid content in indigenous isolates of Ganodermalucidum. Asian J. Pharm. Ana. 2016; 6(4): 213-215.
11.    Valli G, Jeyalakshmi M. Preliminary Phytochemical and Antioxidant Study of Odina woodier Leaf Extract. Asian J. Pharm. Res.2012;  2(4): 153-155.
12.    Sultana K, Kizhekkedath J, Pandey MC. Evaluation of Antioxidant Activity, Radical Scavenging, and Reducing Power of Clove Oil and Clove Oleoresin in Comparison with Natural and Synthetic Antioxidants in Chevon (Capra aegagrus hircus) and Chicken Meat. J.Def. Life. Sci. 2017; 3: 51-58.
13.    Muthukumaran P, Salomi S, Umamaheshwari R. In vitro Antioxidant Activity of Premnaserratifolia Linn. Asian J. Res. Pharm. Sci. 2013; 3(1): 15-18
14.    AraujoJ, Zhang M, Yin F. Heme Oxygenase-1, Oxidation, Inflammation, and Atherosclerosis. J. Front. Pharmacol.  2012; 3: 1-17.
15.    Choi AM, Alam J. Heme Oxygenase-1: Function, Regulation, and Implication of a Novel Stress-inducible Protein in Oxidant-induced Lung Injury. Respir. Cell. Mol. Biol. 1996; 1: 9-19.
16.    Gabr SK, Bakr RO, Mostafa ES, El-Fishawy AM, El-Alfy TS. Antioxidant Activity and Molecular Docking Study of Erythrina × Neillii Polyphenolics. South African Journal of Botany. 2019; 121: 470-477
17.    Shrivastava A, Parveen N, Dewangan G, Verma VS, Bhattacharya A, Sharma M. Evaluation of Anti-Pyretic Potential of Cassia alata Leaves. Asian J. Pharm. Res. 2014; 4(4): 195-197.
18.    Sagnia B, Fedeli D, Casetti R, Montesano C, Falcioni G, Colizzi V. Antioxidant and Anti-Inflammatory Activities of Extracts from Cassia alata, Eleusine  indica, Eremomastax Speciosa, Carica Papaya and Polyscias Fulva Medicinal Plants Collected in Cameroon. PLoS One 2014; 9.
19.    Lewis A, Levy A. Anti-inflammatory Activities of Cassia Alata Leaf Extract in Complete Freund’s  Adjuvant Arthritis in Rats. J. West. Indian. Med.  2011; 60: 615-621.
20.    Saito ST, Trentin D, Macedo AJ, Pungartnik C, Gosmann G, Silveira J, Guecheva TN, Hendriques JAP,  Brendel M. Bioguided Fractionation Shows Cassia alata Extract to Inhibit Staphylococcus Epidermidis and Pseudomonas Aeruginosa Growth and Biofilm Formation. Evidence-Based Complement Altern. Med. 2012; 867103: 1-13
21.    Somchit MN, Reezal I, Nur IE, Mutalib AR. In Vitro Antimicrobial Activity of Ethanol and Water Extracts of Cassia Alata. J. Ethnopharmacol. 2003; 84: 1-4.
22.    Uwazie JN, Yakubu MT, Ashafa AOT, Ajiboye TO. Identification and Characterization of Anti-diabetic Principle in Senna Alata (Linn.) Flower Using Alloxan-Induced Diabetic Male Wistar Rats. J. Ethnopharmacol. 2020; 261: 1-15.
23.    Varghese GK, Bose LV, HabtemariamS. Antidiabetic Components of Cassia Alata Leaves: Identification Through α-Glucosidase  Inhibition Studies. Pharm. Biol. 2013; 51: 345-349.
24.    Sarkar B, Khodre S, Patel P, Mandaniya M. HPLC Analysis And Antioxidant Potential Of Plant Extract Of Cassia Alata J. Asian. Pharm. Sci. Technol. 2014; 4: 4-7.
25.    Pamulaparthi A, Prathap VR, Banala M, Nanna RS. Total Phenolic, Flavonoid Contents and Antioxidant Assays in Leaf Extracts of Senna Alata (L.) Roxb. J. Pharm. Sci & Res.  2015; 8: 981-985.
26.    Kaur C, Kapoor HC. Anti-oxidant Activity and Total Phenolic Content of Some Asian Vegetables. IntJ. Food. Sci.  Technol. 2002; 37: 153-161.
27.    Hemalatha K, Girija K. Evaluation of Drug Candidature of some Benzimidazole Derivatives as Biotin Carboxylase Inhibitors: Molecular docking and Insilico studies. Asian J. Res. Pharm. Sci. 2016; 6(1): 15-20. DOI: 10.5958/2231-5659.2016.00002.3.
28.    Zhang Q, Lin L, Ye W. Techniques for Extraction and Isolation of Natural Products: a Comprehensive review. Chin. Med. 2018; 3: 1-26
29.    Angelina M, Mardhiyah A, Dewi RT, Fajriah S, Muthiah N, Ekapratiwi Y, Dewijanti IID, Sukirno, Jamilah, Hartati S. Physicochemical and Phytochemical Standardization, and Antibacterial Evaluation of Cassia Alata Leaves from Different Locations in Indonesia. Pharmacia, 2021; 68: 947–956.
30.    Buenger J, Ackermann H, Jentzsch A, Mehling A, Pfitzner I, Reiffen KA. An interlaboratory Comparison of Methods used to Assess Antioxidant Potentials. Inter.J. Cosmet.Sci. 2006; 28: 135-146.
31.    Roy A, Bhoumik D, Sahu RK, Dwivedi J. Phytochemical Screening and Antioxidant Activity of Sesbaniagrandiflora Leaves Extracts. Asian J. Res. Pharm. Sci. 214; 4(1): 16-21
32.    Kosalec I, Bakmaz M, Pepeljnjak S, Vladimir-Knezević S. Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta. Pharm. 2004; 54: 65-72.
33.    Kulkarni K, Govindaiah. Evaluation of Anti-oxidant properties of some medicinal plant products by ABTS Radical Scavenging Assay. Research Journal of Science and Technology. 2022; 14(4): 213-8.
34.    Ahmed D, Zara S, Baig H. In Vitro Analysis of Antioxidant Activities of Oxalis Corniculata Linn. Fractions in Various Solvents. J. Afr. Tradit. Complement. Altern. Med. 2012; 10: 158-165.
35.    Rangasamy P, Hansiya VS, Maheswari PU, Suman T, Geetha N. Phytochemical Analysis and Evaluation of In vitro Antioxidant and Anti-urolithiatic Potential of various fractions of Clitoriaternatea L. Blue Flowered Leaves. Asian J. Pharm. Ana. 2019; 9(2): 67-76. DOI: 10.5958/2231-5675.2019.00014.0.
36.    Abriyani E, Fikayuniar L. Screening Phytochemical, Antioxidant Activity and Vitamin C Assay from Bungoperak-perak (Begonia versicolar Irmsch) leaves. Asian J. Pharm. Res. 2020; 10(3):183-187. DOI: 10.5958/2231-5691.2020.00032.5.
37.    Sadeghi Z, Valizadeh J, Shermeh OA, Akaberi M. Antioxidant Activity and Total Phenolic Content of Boerhavia Elegans (choisy) Grown in Baluchestan, Iran. J. Avicenna. Phytomedicine. 2015; 5: 1-9.
38.    Loizzo MR, Pugliese A, Bonesi M, Menichini F, Tundis R. Evaluation of Chemical Profile and Antioxidant Activity of Twenty Cultivars from Capsicum Annuum, Capsicum Baccatum, Capsicum Chacoense and Capsicum Chinense: A comparison Between Fresh and Processed Peppers. LWT – Food. Sci. Technol 2015; 64: 623-631.

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