Author(s): Vikas D. Kedar, Pritam N. Dube, Sachin N. Kapse, Yogita B. Thombare

Email(s): kedarvikas10@gmail.com

DOI: 10.52711/0974-360X.2024.00252   

Address: Vikas D. Kedar*, Pritam N. Dube, Sachin N. Kapse, Yogita B. Thombare
Research Scholar Matoshri College of Pharmacy Ekalahare, Nashik. 422105 Maharashtra.
*Corresponding Author

Published In:   Volume - 17,      Issue - 4,     Year - 2024


ABSTRACT:
A simple, rapid, accurate and sensitive method was developed for quantitative analysis of Trospium Chloride in tablet dosage form using Reverse Phase high performance liquid chromatography (RP-HPLC) with DAD detection. The chromatography separation was achieved Xterra RP18, (250 x 4.6mm, 5µParticle Size)), at column temperature 40°C±2, in the isocratic mobile phase mode using Acetonitrile (ACN): Buffer (65:35; v/v) at a flow rate of 1.0mL/min. The determination was performed using Buffer HPLC with DAD detector set at 215nm. Samples were prepared with diluent [Buffer: ACN (200:100; v/v)], and the volume injected was 50µL. The retention time for Trospium Chloride was 3.275 minute. The analytical curve was linear (r2 0.9993) over a wide concentration range (50.0-150.0 µg/mL). The presence of components of the tablet did not interfere in the results of the analysis. The method showed adequate precision with a relative standard deviation (RSD) less than 2%.


Cite this article:
Vikas D. Kedar, Pritam N. Dube, Sachin N. Kapse, Yogita B. Thombare. Development and Validation of Stability indicating RP-HPLC Assay Method for Trospium Chloride in Tablet Dosage Form. Research Journal of Pharmacy and Technology.2024; 17(4):1595-8. doi: 10.52711/0974-360X.2024.00252

Cite(Electronic):
Vikas D. Kedar, Pritam N. Dube, Sachin N. Kapse, Yogita B. Thombare. Development and Validation of Stability indicating RP-HPLC Assay Method for Trospium Chloride in Tablet Dosage Form. Research Journal of Pharmacy and Technology.2024; 17(4):1595-8. doi: 10.52711/0974-360X.2024.00252   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-4-26


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DOI: 10.5958/0974-360X 

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