Author(s): Nutan Kendre, Pravin Wakte

Email(s): nutank.pharma23@gmail.com

DOI: 10.52711/0974-360X.2024.00227   

Address: Nutan Kendre*, Pravin Wakte
University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431004, Maharashtra, India.
*Corresponding Author

Published In:   Volume - 17,      Issue - 4,     Year - 2024


ABSTRACT:
Natural products, particularly those derived from plants and microbes, offer an endless supply of unique compounds that can be converted into new pharmaceuticals. Due to their potential health benefits, bioactive components from herbs, particularly phenolics, have recently attracted a lot of interest. One of the multi-use forest tree species, Madhuca longifoliaJ. Koenig. (mahua), is found throughout much of South Asia and offers locals a source of food, fuel, fodder, and other non-timber forest products (NTFPs) in addition to timber. Almost all of this tree's components exhibit its therapeutic qualities.Mahua's phytochemical analysis reveals that it contains high levels of vitamins, alkaloids, sugar, glycosides, proteins, steroids, tannins, flavonoids, saponins, terpenoids, and phenolic compounds. These compounds are responsible for a variety of pharmacological properties, including anti-inflammatory, antioxidant, analgesic, antihyperglycemic, spasmolytic, hepatoprotective, anticonvulsant, anticancer, results of the High-Resolution Liquid Chromatography Mass Spectroscopy (HR-LCMS) analysis showed that plant extracts included a number of pharmaceutically significant chemicals. By comparing the weights and fragmentation patterns of the twenty-four (24) phenolic compounds in this work with those of published libraries, the LC-MS/MS method was used to screen and confirm them. The findings of this research support the usage of this plant as a source of bioactives and the beneficial effects of these compounds on human health. Madhuca longifolia, HR-LCMS, Bioactives, Natural products, Phenolic compounds.


Cite this article:
Nutan Kendre, Pravin Wakte. LC-MS/MS-QTOF Screening and Identification of Phenolic compounds from Ethyl Acetate Fraction of Madhuca longifolia Leaves. Research Journal of Pharmacy and Technology.2024; 17(4):1435-0. doi: 10.52711/0974-360X.2024.00227

Cite(Electronic):
Nutan Kendre, Pravin Wakte. LC-MS/MS-QTOF Screening and Identification of Phenolic compounds from Ethyl Acetate Fraction of Madhuca longifolia Leaves. Research Journal of Pharmacy and Technology.2024; 17(4):1435-0. doi: 10.52711/0974-360X.2024.00227   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-4-1


REFERENCES:
1.    Rupa Bhattacharya, Prajakta Naitam. Green Anticancer Drugs – A Review. Res. J. Pharmacognosy and Phytochem. 2019; 11(4): 231-243.
2.    Savoia D. Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol. 2012; 7: 979–990. https://doi. org/10.2217/fmb.12.68.
3.    Suryawanshi and Mokat. IJPSR. 2019; 10(2): 786-789.
4.    Jerine Peter S, Ram Kumar K, Manisha P, Sangeetha N, Arun Raj N, Usha Kumari, Evan Prince Sabina. Potential activity of Madhuca longifolia leaf extract: through In vitro, Pharmacological and In-silico studies. Research J. Pharm. and Tech. 2020; 13(3): 1083-1091.
5.    Jerine Peter S, Nagesh Kishan Panchal, Mary Thomas, Gayathri Ashok, Megha Treesa Saju, Padma Thiagarajan, Evan Prince Sabina. Gastrotoxicity gene modelling and its binding affinity of docked complex with the ligands of Madhuca longifolia. Research J. Pharm. and Tech. 2020; 13(12):5799-5805.
6.    Pragati Khare, Kamal Kishore, Dinesh Kumar Sharma. Catalase and Superoxide Dismutase (SOD) activity in Swiss albino mice treated with ethanolic leaf extract of Madhuca longifolia. Research J. Pharm. and Tech. 2019; 12(9):4434-4437.
7.    M Umadevi, C Maheswari , R Jothi, Sai Kishore Paleti, Y Srinivasa Reddy, R Venkata Narayanan. Hepatoprotective Activity of Flowers of Madhuca longifolia (Koen.) Macbr. Against Paracetamol-Induced Hepatotoxicity. Research J. Pharm. and Tech. 2011; 4(2): 259-262.
8.    Kendre N, Wakte P. A review on Phytochemicals and biological attributes of Madhuca longifolia. AJPP. 2021; 7(2):74-84.
9.    Hussain, S.A., Panjagari, N.R., Singh, R.R.B., Patil, G.R. Potential herbs and herbal nutraceuticals: Food applications and their interactions with food components. Crit. Rev. Food Sci. Nutr. 2015; 55: 94–122.
10.    Aktar ali et al. LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential. Antioxidants. 2021; 10: 1770. https://doi.org/10.3390/ antiox10111770.
11.    Kumar, S.; Pandey, A.K. Chemistry and biological activities of flavonoids: An overview. Sci. World J. 2013; 162750.
12.    Manish Devgun, Arun Nanda, SH Ansari, SK Swamy. Recent Techniques for Extraction of Natural Products. Research J. Pharm. and Tech. 2010; 3(3): 644-649.
13.    Salunke M, Wakure B, Wakte P. Phytochemical analysis of Acanthophora najadiformis using High-Resolution Liquid Chromatography Mass Spectrometry (HR-LCMS) and FTIR. Journal of Pharmaceutical Negative Results. 2022, 13(6): 2215–2218. https://doi.org/10.47750/pnr.2022.13.S06.287
14.    Salunke M, Wakure B, Wakte P. HR-LCMS assisted phytochemical screening and an assessment of anticancer activity of Sargassum Squarrossum and Dictyota Dichotoma using in vitro and molecular docking approaches. J Mol Struct. 2022a, 1270. https://doi.org/10.1016/j.molstruc.2022.133833
15.    Salunke M, Wakure B, Wakte P. High-resolution liquid chromatography mass spectrometry (HR-LCMS) and 1H NMR analysis of methanol extracts from marine seaweed Gracilaria edulis. Nat Prod Res. 2022b, 1–4. https://doi.org/10.1080/14786419.2022.2146906
16.    Salunke M, Wakure B, Wakte P. Phytochemical Screening of Marine Brown Algae Sargassum squarrossum Greville. Bull EnvPharmacol Life Sci. 2022c, 11(2):112–116.
17.    Bhuyan DJ, Basu A. Phenolic compounds: potential health benefits and toxicity. In: Vuong QV (ed) Utilisation of bioactive compounds from agricultural and food production waste. CRC Press, Taylor & Francis Group, Boca Raton, 2017, pp 27–59.
18.    Deeparani K. Urolagin, S. Jayakumari. In vitro Anti-Cancer Study of Vitis Viniferae, Ixora Coccinea and Piper Longum Ethanolic extracts on Human Breast Carcinoma Cells. Research J. Pharm. and Tech. 2018; 11(12): 5345-5347.
19.    Neupane P, Lamichhane J. Estimation of total phenolic content, total favonoid content and antioxidant capacities of five medicinal plants from Nepal. Vegetos. 2020, https://doi.org/10.1007/s42535-020- 00116-7.
20.    Jurga Bernatoniene et al. Flavonoids as anticancer agents. Nutrients. 2020, 12:457; doi:10.3390/nu12020457.
21.    Swarnalatha. Y. Isolation of Flavonoids and their Anticancer Activity from Sphaeranthus amaranthoides in A549 Cell Line. Research J. Pharm. and Tech. 2015; 8(4): 462-467.
22.    Nazia Afroze et al. A review on myricetin as a potential therapeutic candidate for cancer prevention. 3 Biotech. 2020, 10:211. https://doi.org/10.1007/s13205-020-02207-3.
23.    Kim ME, Ha TK, Yoon JH, Lee JS. Myricetin induces cell death of human colon cancer cells via BAX/BCL2-dependent pathway. Anticancer Res. 2014; 34(2):701–706.
24.    Ma L t al. Discovery of myricetin as a potent inhibitor of human fap endonuclease 1, which potentially can be used as sensitizing agent against HT-29 human colon cancer cells. J Agric Food Chem. 2019. https://doi. org/10.1021/acs.jafc.8b05447.
25.    Sun W et al. Myricetin exerts potent anticancer effects on human skin tumor cells. Trop J Pharmaceut Res. 2018; 17(6): 1067–1072. https://doi.org/10.4314/tjpr. v17i6.13.
26.    Li-dong Chen et al. Sanggenon C induces apoptosis of colon cancer cells via inhibition of NO production, iNOS expression and ROS activation of the mitochondrial pathway. Oncology Reports. 2017; 38: 2123-2131. DOI: 10.3892/or.2017.5912
27.    Do-Young yoon et al. Trifolin induces apoptosis via extrinsic and intrinsic pathways in the NCI-H460 human non-small cell lung-cancer cell line. Phytomedicine. 2016. doi: 10.1016/j.phymed.2016.05.009
28.    R. Elanthendral, K. Gayathri. Preliminary Phytochemical Analysis and Chemical Characterization of Acetone Extract from Tender Coconut Mesocarp. Research J. Pharm. and Tech. 2019; 12(12): 5781-5785.
29.    Ganadhal Puttaramaiah Chethankumara, Venkatarangaiah Krishna, Kakanahalli Nagaraj. HR-LCMS and In vitro cytotoxicity analysis of Alseodaphne semecarpifolia stem bark and leaf methanol extracts. Research Journal of Pharmacy and Technology. 2022; 15(1):250-6.
30.    T M Kalyankar, A C Jadhav, S M Mhetre. Recent Advances in Coupling Technology in Analysis of Natural Product. Research J. Pharm. and Tech. 2012; 5(9): 1145-1153.
31.    Osswald S. Havel M. Gogotsi Y. Monitoring oxidation of multiwalled carbon nanotubes by Raman spectroscopy. Journal of Raman Spectroscopy An International Journal for Original Work in all Aspects of Raman Spectroscopy, Including Higher Order Processes, and also Brillouin and Rayleigh Scattering. 2007; 38(6):728–736. doi.org/10.1002/jrs.1686

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank


Recent Articles




Tags


Not Available