Author(s):
Tahid Alam, Bapi Ray Sarkar, Pooja Sharma Luitel, Arup kumar Gorai
Email(s):
tahidalam108@gmail.com
DOI:
10.52711/0974-360X.2024.00874 
Address:
Tahid Alam*, Bapi Ray Sarkar, Pooja Sharma Luitel, Arup kumar Gorai
Department of Pharmaceutical Technology, University of North Bengal, Raja Rammohunpur, Darjeeling, West Bengal-734013, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 12,
Year - 2024
ABSTRACT:
Natural drugs are one of the most important needs in daily life and their history is as old as human civilization. Orchids are nature's most beautiful and widely dispersed flowering plant groupings. Cymbidium aloifolium (L) SW family (Orchidaceae) is an epiphytic herbaceous orchid. The orchidaceae family is one of the oldest and largest flowering plant families in the world, with over 800 genera and between 25000 and 35000 species. The current study aims to examine in vitro antioxidant activity from different solvent extracts of Cymbidium aloifolium (L) SW leaves. The examination was conducted by several factors, including pharmacognostical, physicochemical, and phytochemical details of the leaf extracts of Cymbidium aloifolium (L) SW, which plays a vital role in laying down standardization and pharmacopoeia parameters. The antioxidant capacity of various solvent extracts of Cymbidium aloifolium (L) SW leaf extract was investigated using ferric reducing antioxidant potential (FRAP) tests and DPPH radical scavenging activities. A microscopic investigation exposes the leaf's cellular structure. To standardize the aim, physicochemical investigations were established, including swelling index, fluorescence analysis, total ash, acid insoluble ash, water soluble ash, and loss on drying. Antioxidant potential was determined by comparing the results with ascorbic acid standard. Leaves of Cymbidium aloifolium (L) SW methanolic extract showed the best result, followed by chloroform, ethyl acetate, pet ether, and water. These findings support the identification of Cymbidium aloifolium (L) SW and quality control measures. Further investigation is required to identify the specific phytochemical and the way in which it generates antioxidant activity.
Cite this article:
Tahid Alam, Bapi Ray Sarkar, Pooja Sharma Luitel, Arup kumar Gorai. Pharmacognostical parameters, sphytochemical screening, TPC, TFC and evaluation of in-vitro anti-oxidant activity of leaves of Cymbidium aloifolium L (SW). Research Journal Pharmacy and Technology. 2024;17(12):5743-9. doi: 10.52711/0974-360X.2024.00874
Cite(Electronic):
Tahid Alam, Bapi Ray Sarkar, Pooja Sharma Luitel, Arup kumar Gorai. Pharmacognostical parameters, sphytochemical screening, TPC, TFC and evaluation of in-vitro anti-oxidant activity of leaves of Cymbidium aloifolium L (SW). Research Journal Pharmacy and Technology. 2024;17(12):5743-9. doi: 10.52711/0974-360X.2024.00874 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-12-11
REFERENCES:
1. Sam S. Importance and effectiveness of herbal medicines. Journal of Pharmacognosy and Phytochemistry. 2019; 8(2): 354-7.
2. Hegde SN. Orchids of Arunachal Pradesh. Forest Department Arunachal Pradesh; 1984.
3. Kumar CS, Shetty BV, Bennet SS, Rao TA, Molur S, Walker S. Endemic orchids of the Western Ghats. In Conservation Assessment and Management Plan (CAMP) Workshop Report 2001.
4. Gutiérrez RM. Orchids: A review of uses in traditional medicine, its phytochemistry and pharmacology. J. Med. Plants Res. 2010; Apr 18; 4(8): 592-638
5. Khasim S, Rao PM. Medicinal importance of orchids. The Botanica. 1999; 49: 86-91.
6. Kasulo V, Mwabumba L, Cry M. A review of edible orchids in Malawi.
7. Potshangbam N, Nirmala C. In vitro rapid propagation of Cymbidium aloifolium (L.) Sw.: a medicinally important orchid via seed culture. Journal of Biological Sciences. 2011; 11(3): 254-60.
8. Rajbhandari KR, Dahal S. Orchids of Nepal: a checklist. Botanica Orientalis: Journal of Plant Science. 2004; 4(1): 89-106.
9. Arora M, Mahajan A, Sembi JK. A review on phytochemical and pharmacological potential of family Orchidaceae. International Research Journal of Pharmacy. 2017; 8(10): 9-24.
10. Xavier TF, Kannan M, Auxilia A. Traditional Medicinal Plants Used in the treatment of different skin diseases. International Journal of Current Microbiology and Applied Sciences. 2015; 4(5): 1043-53.
11. M Prathapa Reddy, T R Shantha, V Rama Rao, G Venkateshwarlu. Pharmacognostical and Physicochemical Evaluation on the Flowers of Justicia adhatoda L. Res. J. Pharmacognosy & Phytochem. 2015; 7(2): 73-90. doi: 10.5958/0975-4385.2015.00014.X
12. Ashish Vaishnav, Anish Chandy, Deenanath Jhade, Sudhish Rai. Pharmacognostical and Preliminary Phytochemical Studies on Moringa olifera Leaves. Research J. Pharmacognosy and Phytochemistry. 2011; 3(6): 272-274.
13. Debashisha Panda, Santosh Kumar Dash, Gouri Kumar Dash. Preliminary phytochemical screening and Pharmacogcostic studies of the common weeds, Mimosa pudica Linn., Chromolaena odorata Linn. and Tragia involucrata Linn. collected from Ganjam District, Odisha: A Comparative Study. Research J. Pharmacognosy and Phytochemistry. 2012; 4(2): 92-96.
14. Sylvester AW, Ruzin SE. Light microscopy I: dissection and microtechnique. InThe Maize Handbook 1994 (pp. 83-95). New York, NY: Springer New York.
15. K. Venkata Ramana, M. Himaja Trivedi, P. Rama Krishna Reddy, T. L. Kalyani, K. Haritha Reddy, S. Mohana Lakshmi. Pharmacognostical Studies on Ficus nervosa Heyne Ex Roth Leaves. Res. J. Pharmacognosy and Phytochem. 2014; 6(2): 84-87
16. Mohammad Shamim Qureshi, A. Venkateshwar Reddy, G.S. Kumar, Lubna Nousheen. Pharmacognostic, Physicochemical Standardization and Phytochemical Analysis of leaves of Hydrolea zeylanica Vahl. (Hydrophyllaceae). Res. J. Pharmacognosy and Phytochem. 2017; 9(1): 01-07. doi: 10.5958/0975-4385.2017.00001.2
17. Mushahida Parveen, Jayshree Narayanan. Pharmacognostical Evaluation of Erythrina stricta Roxb: Stem Bark. Res. J. Pharmacognosy and Phytochem. 2015; 7(3): 141-145. doi: 10.5958/0975-4385.2015.00025.4.
18. Balamurugan Gunasekaran, P. Muralidharan, A. Pandiselvi, P. Amutha. Preliminary Phytochemical screening and Anti oxidant activities of Ethanolic extract of Caesalpinia sappan Linn. Research J. Pharm. and Tech. 2008; 1(3): 179-181.
19. WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. World Health Organization. WHO Press, Geneva, Switzerland, 2007;
20. Gunde MC, Suruse PB, Amnerkar ND, Lohe RW, Godbole MD, Kale MK, Pathak AK. Antioxidant Activity of Tubers of Arisaema Leschenaultii Blume. Research J. Pharm. and Tech. 2009; 2(1): 134-137
21. Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. 2010; 3(2): 551-555
22. Jennifer Suganthi S., Uma Devi P., Suba Kanmani S. Free Radical Scavenging Potential and HPTLC profile of Mimosa pudica. Research J. Pharm. and Tech. 2011; 4(7): 1090-1094
23. Himesh Soni, Govind Nayak, Kaushelendra Mishra, A.K. Singhai, A.K. Pathak. Evaluation of Phyto Pharmaceutical and Antioxidant Potential of Methanolic Extract of Peel of Punica granatum. Research J. Pharm. and Tech. 2010; 3 (4): 1170-1174.