ABSTRACT:
Mangiferin is a naturally occurring antioxidant that belongs to a glucoxyl xanthone and has potential therapeutic activity with no side effects. It is mostly available from the leaves and stems of the Mangifera indica (mango tree). To acquire, track, and analyse the output chromatogram data, the analytical method was created using the Shimadzu HPLC system equipped with a PDA detector and the LC Solution 5.57 system control software. Phenomenex Kinetex® 5 microns C18 (250 x 4.6mm)Column was used as stationary phase to separate the drug. Isocratic elution was obtained with 10mM phosphate buffer pH-2.4 along with 0.1% triethylamine and acetonitrile mixture as a mobile phase in the ratio of 90:10% v/v with the flow rate of 1.3ml per minute at 258nm, which appears to be fair based on the reported run time. The calibration plot for Mangiferin in the range between 100ng/mL - 25µg/mL showed good linearity with 0.999R2 value and the validation was successfully completed as per ICH guidelines. The peak % relative standard deviation (RSD) for robustness testing followed the acceptance criteria. Finding the concentration of mangiferin in dosage forms and bulk was made easy, accurate, exact, and affordable with this procedure.