Author(s):
Indah Yulia Ningsih, Ilham Robbynoor Sulistyono, Zidni Hafizha, Alwi Robiyanto, Endah Puspitasari, Dewi Dianasari, Mochammad Amrun Hidayat
Email(s):
indahyulianingsih.farmasi@unej.ac.id
DOI:
10.52711/0974-360X.2024.00854 
Address:
Indah Yulia Ningsih1*, Ilham Robbynoor Sulistyono2, Zidni Hafizha3, Alwi Robiyanto3, Endah Puspitasari4, Dewi Dianasari4, Mochammad Amrun Hidayat1
1Biomaterials and Bioproducts Research Group, Faculty of Pharmacy, University of Jember, Jember 68121, East Java, Indonesia.
2Stikes Borneo Cendekia Medika, West Kotawaringin 74111, Central Kalimantan, Indonesia.
3Undergraduate Student, Faculty of Pharmacy, University of Jember, Jember 68121, East Java, Indonesia.
4Bioactive Natural Products Development Research Group, Faculty of Pharmacy, University of Jember, Jember 68121, East Java, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 11,
Year - 2024
ABSTRACT:
Eleutherine americana (Aubl.) Merr. is used as a traditional remedy in Indonesia. Based on its chemical compounds, the leaves were expected to exhibit antibacterial and antioxidant activities. The aim of the study was to assess the antibacterial capacity, antioxidant capacity, total phenolic content, total flavonoid content, and phytochemical compounds in ethanolic extract of E. americana leaves collected from various cultivation sites, namely West Kotawaringin, Central Kalimantan (EW); Malang, East Java (EM); and Probolinggo, East Java (EP). The antibacterial activity assay was conducted utilizing the diffusion method, whereas the determination of antioxidant capacity was performed utilizing the DPPH method. The total phenolic content and total flavonoid content were determined using UV-Vis spectrophotometry. The phytochemical screening was conducted utilizing a tube test and thin layer chromatography (TLC) method. The growth of E. coli and S. aureus was found to be inhibited by EW, EM, and EP. Furthermore, the antibacterial activity of all samples against S. aureus was higher than that against E. coli. EP had the highest antioxidant activity (IC50 = 32.83±3.51 µg/mL), total phenolic content of 129.40±0.19 mg GAE/g extract, and total flavonoid content of 0.61±0.01 mg QE/g extract. Phytochemical screening revealed that the leaves of E. americana contained alkaloids, terpenoids, flavonoids, polyphenols, and tannins. According to the findings of this study, E. americana leaves could potentially be developed as a new natural antibacterial and antioxidant agent.
Cite this article:
Indah Yulia Ningsih, Ilham Robbynoor Sulistyono, Zidni Hafizha, Alwi Robiyanto, Endah Puspitasari, Dewi Dianasari, Mochammad Amrun Hidayat. Antibacterial and antioxidant activities of Eleutherine americana (Aubl.) Merr. leaves extract growing in different cultivation sites. Research Journal of Pharmacy and Technology. 2024; 17(11):5597-4. doi: 10.52711/0974-360X.2024.00854
Cite(Electronic):
Indah Yulia Ningsih, Ilham Robbynoor Sulistyono, Zidni Hafizha, Alwi Robiyanto, Endah Puspitasari, Dewi Dianasari, Mochammad Amrun Hidayat. Antibacterial and antioxidant activities of Eleutherine americana (Aubl.) Merr. leaves extract growing in different cultivation sites. Research Journal of Pharmacy and Technology. 2024; 17(11):5597-4. doi: 10.52711/0974-360X.2024.00854 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-11-62
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