Author(s):
Yufri Aldi, Dita Permatasari, Sera Afdalanita, Aditya Alqamal Alianta
Email(s):
yufrialdi@gmail.com , yufrialdi@phar.unand.ac.id , ditapermatasari@phar.unand.ac.id , seraafda@gmail.com , adityaalqamal@ansci.unand.ac.id
DOI:
10.52711/0974-360X.2024.00845 
Address:
Yufri Aldi1, Dita Permatasari2, Sera Afdalanita3, Aditya Alqamal Alianta4
1Faculty of Pharmacy, Universitas Andalas, Pauh, Padang, West Sumatra, Indonesia.
2Faculty of Pharmacy, Universitas Andalas, Pauh, Padang, West Sumatra, Indonesia.
3Faculty of Pharmacy, Universitas Andalas, Pauh, Padang, West Sumatra, Indonesia.
4Faculty of Animal Science, Universitas Andalas, Pauh, Padang, West Sumatra, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 11,
Year - 2024
ABSTRACT:
Introduction: Moringa oleifera Lam. is widely used in medicine by the community to increase body endurance. Aim: The study aimed to determine the safety of Moringa leaf extract against kidney histology on repeated use. Material and Methods: A total of 36 male white rats aged 2-3 months were divided into four groups, namely one control group, which was given 1% Na CMC and three treatment groups, which were given Moringa leaf ethanol extract at doses of 7, 21, and 140mg/kg BW for 7, 14, and 21 days. Animals were sacrificed on days 8, 15, and 22; their kidneys were collected. The parameters namely descriptively presented renal histology and the ratio of kidney organs were analysed, using two-way ANOVA. Result: The results demonstrated that the group with the highest dose (140mg/kg BW) showed an average increase in damage scores, including 1.4, 1.6, and 2.4 (minimal-moderate damage). Accordingly, based on the duration of administration, the histology scores showed increasing numbers on days 7, 14 and 21 with successive averages of 0.55, 0.7 and 1.25. The results of the ratio of kidney parts showed a decrease which was influenced by the duration of administration (p<0.05) and was not affected by the dose (p>0.05). The results of Duncan's follow-up test showed no significant difference between each group for the duration of administration (p>0.05). Based on this, it can be concluded that the Moringa leaves did not significantly damage the kidney.
Cite this article:
Yufri Aldi, Dita Permatasari, Sera Afdalanita, Aditya Alqamal Alianta. Safety Evaluation of Moringa Leaves (Moringa oleifera Lam.) on Kidney Organs in Male White Rats. Research Journal of Pharmacy and Technology. 2024; 17(11):5531-9. doi: 10.52711/0974-360X.2024.00845
Cite(Electronic):
Yufri Aldi, Dita Permatasari, Sera Afdalanita, Aditya Alqamal Alianta. Safety Evaluation of Moringa Leaves (Moringa oleifera Lam.) on Kidney Organs in Male White Rats. Research Journal of Pharmacy and Technology. 2024; 17(11):5531-9. doi: 10.52711/0974-360X.2024.00845 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-11-53
REFERENCES:
1. Elfahmi. Woerdenbag HJ. Kayser O. Jamu: Indonesian traditional herbal medicine towards rational phytopharmacological use. Journal of Herbal Medicine. 2014; 4(2): 51–73. https://doi.org/10.1016/j.hermed.2014.01.002
2. Surya, R., Romulo, A., Nurkolis, F., Kumalawati, D.A.. Compositions and Health Benefits of Different Types of Jamu, Traditional Medicinal Drinks Popular in Indonesia. In: Mérillon, JM., Riviere, C., Lefèvre, G. (eds) Natural Products in Beverages. Reference Series in Phytochemistry. Springer, Cham. 2024. https://doi.org/10.1007/978-3-031-04195-2_123-1
3. World Health Organization (WHO). General guidelines for methodologies on research and evaluation of traditional medicine. No. WHO/EDM/TRM/2000.1. World Health Organization, 2000.
4. Rivai, ATO. Identifikasi Senyawa yang Terkandung pada Ekstrak Daun Kelor (Moringa oleifera). Indonesian Journal Fundamental Sciences. 2020; 6(2): 63–70. https://dx.doi.org/10.26858/ijfs.v6i2.16870
5. Lam CS. Koon HK. Chung VC-H. Cheung YT. Id C. A public survey of traditional, complementary and integrative medicine use during the COVID-19 outbreak in Hong Kong. PloS ONE 16(7): e0253890. 2021; 1–15. http://dx.doi.org/10.1371/journal.pone.0253890
6. Jayanti RD. Wittiarika ID. Amalia RB. Winardi B. Winarsih S. Wiyasa IWA. The Effect of Moringa Leaves Aqueous Extract to Ovarian Sodium Dismutase and Apoptotic Index in Rats Treated With Depomedroxyprogesterone Acetate. Research Journal Pharmacy Technology. 2023; 16(5): 2103–2106. https://doi.org/10.52711/0974-360X.2023.00345
7. Dillasamola D. Aldi Y. Fakhri M. Diliarosta S. Biomechy OP. Noverial. Immunomodulatory effect test from moringa leaf extract (Moringa oleifera l.) with carbon clearance method in male white mice. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(9): 241–245. https://doi.org/10.22159/ajpcr.2018.v11i9.26703
8. Fathir A, Rifa’i M. Widodo. Aktivitas Ekstrak Daun Kelor Terhadap Sel-T Helper dan Sel-T Sitotoksik pada Mencit yang Diinfeksi Salmonella THYPI. Jurnal Veteriner. 2014; 15(1): 114–122. https://doaj.org/article/a3c10afb472b4f359c1e85aa1e763dee
9. Nararya SA. Jularso E. Budhy TI Uji Toksisitas Daun Kelor (Moringa Oleifera) Terhadap Sel Fibroblas Gingiva Menggunakan Uji MTT Assay. Jurnal Biosains Pascasarjana. 2015; 17(1): 52-59. https://doi.org/10.20473/jbp.v17i1.2015.52-58
10. Tamimi AAP, de Queljoe E, Siampa JP. Uji Efek Analgesik Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus). Pharmacon. 2020; 9(3): 325-333. https://doi.org/10.35799/pha.9.2020.30015
11. Putra IWDPP. Dharmayudha AAGO. Sudimartini LM. Identifikasi Senyawa Kimia Ekstrak Etanol Daun Kelor (Moringa oleifera L) di Bali (Identification of Chemical Compounds Ethanol Extract Leaf Moringa (Moringa oleifera L) In Bali). Indonesia Medicus Veterinus Oktober. 2016; 5(5): 464–473. Retrieved https://arkn-fpd.org/data_content/hmp_directory/Indonesia_Komposisi_Kimia_Kelor.pdf
12. Widyaningsih TD. Sari RA. Rukmi WD. Dahniar SA. The Effect of Moringa Leaf (Moringa oleifera Lam.), Pandan Wangi (Pandanus amaryllifolius Roxb.) and Red Ginger (Zingiber officinale Rosc. Var) Extract Supplement Intervention on Uric Acid, Kidney, Liver Function and Perceptions of Hyperuricemia patients on health benefit. Research Journal Pharmacy Technology. 2022; 15(10): 4477–4484. http://dx.doi.org/10.52711/0974-360X.2022.00751
13. Gbankoto A. Sindete M. Adjagba M. Sangare MM. Attakpa ES, Awede B. Antihypertensive effects of Moringa oleifera leaf extract Lam. (Moringaceae) in NG-nitro-L-arginine-methyl ester-induced hypertensive rats. National Journal of Physiology Pharmacy Pharmacology. 2019; 9(12): 1257-1266. http://dx.doi.org/10.5455/njppp.2019.9.1034231102019
14. Mondal C, Chandra AK. Goitrogenic antithyroidal potential of moringa leaves (Moringa oleifera) and spinach (spinacia oleracea) of indian origin on thyroid status in male albino rats. Brazilian Journal of Pharmaceutical Sciences. 2019; 55: 1–11. https://doi.org/10.1590/s2175-97902019000218005
15. Syamra A, Indrawati A, Warsyidah AA. Pemberian Rebusan Daun Kelor Terhadap Penurunan Kadar Glukosa Darah Pada Pasien Penderita Diabetes Mellitus (DM). Jurnal Media Laboran. 2018; 8(2): 50-55. Retrieved from https://jurnal.uit.ac.id/MedLAb/article/view/464
16. Bhowmik D. Chiranjib. Dubey P. Chandira M. Kumar KPS. Herbal drug toxicity and safety evaluation of traditional medicines. Scholars Research Library. 2011; 2(4): 373–383. http://scholarsresearchlibrary.com/AASR-second-issue/5.AASR1_2_32-56.pdf
17. Berglund. Anaphylaxis to Moringa oleifera: First description. Australasian Medical Journal. 2018; 11(3): 176–177. https://doi.org/10.21767/AMJ.2018.3344
18. Rim L. Ahn S. Ha SY. Koo C. Kang JT. Acute Pancreatitis Induced by Moringa oleifera in a 48 years Old Korean Women : A Case Report. Journal of The Korean Society of Emergency Medicine. 2015; 26(6): 612–6125.
19. Olanrewaju FO. Onayemi O. Olasode OA. Oripelaye MM. Fixed drug eruption induced by moringa oleifera leaf extracts – A case report. Our Dermatology Online. 2017; 8(4): 413–416. http://dx.doi.org/10.7241/ourd.20174.117
20. Suman. Herbs: An Alternative Approach in Nephroprotection. Research Journal Pharmacognosy and Phytochemistry. 2013; 5(1): 15–21. Available on: https://rjpponline.org/AbstractView.aspx?PID=2013-5-1-3
21. Homady MH. Majeed AA Histochemical and Pheromonal Components of Mouse Preputial Gland in Relation to F. hormonis Treatment. Research Journal of Pharmacy and Technology. 2017; 10(12): 4137–4141. http://dx.doi.org/10.5958/0974-360X.2017.00753.3
22. RI K. Keputusan Menteri Kesehatan Republik Indonesia Nomor 381/MENKES/SK/III/2007 tentang Kebijakan Obat Tradisional Nasional. Jakarta: Menteri Kesehatan Republik Indonesia; 2007. Available on https://bikinpabrik.id/wp-content/uploads/2019/03/Buku-Kebijakan-Obat-Tradisional-Nasional-Tahun-2007.pdf
23. BPOM RI. Peraturan Badan Pengawas Obat Dan Makanan Tentang Pedoman Uji Toksisitas Praklinik Secara in Vivo. 2022. Available on https://jdih.pom.go.id/download/product/1379/10/2022
24. Permatasari D. Oktavia I. Nazar A. Ahmadin A. The Sub Acute Toxicity Study of Purified Gambir (Uncaria gambir Roxb.) to Liver Histology and its Reversibility on Rats. Bioscience. 2021; 5(1): 12-20. https://doi.org/10.24036/0202151111642-0-00
25. Deepika D. SL Harikumar. Kaur GA. Role of Herbal Drugs in Nephrotoxicity and Neurological Disorder: Connecting a Bridge between Kidney and Brain. Research Journal Pharm and Tech. 2013; Oct; 6(10): 1165–11168. Available on https://rjptonline.org/HTML_Papers/Research%20Journal%20of%20Pharmacy%20and%20Technology__PID__2013-6-10-14.html
26. Pavani P. Naveena E. Subhashini P. Srikant S. Thirumala NK. Kishore BM. A Review on Non Steroidal Anti-Inflammatory Drugs Induced Kidney Diseases. Research Journal of Pharmacology and Pharmacodynamics. 2022; 14(4): 268–272. http://dx.doi.org/10.52711/2321-5836.2022.00046
27. Muthukumaran P. Hazeena BV. Effect of Poorna Chandrodayam Chendooram (PCM - Metallic Drug) on Lipid Profile, Liver Function and Kidney Function Parameters of Rats. Asian Journal Pharmaceutical Analysis. 2020; 10(1): 27–31. http://dx.doi.org/10.5958/2231-5675.2020.00006.X
28. Osman H. Shayoub ME. Babiker EM, Faiza AO, Munzir MEA. Osman B. Elhassan M, Taha KK. Assessment of acute toxicity and LD50 of Moringa oleifera ethanolic leave extract in albino rats and rabbits. Journal of Medical and Biological Science Research. 2015; 1(4): 38–43.
29. Departemen Kesehatan. Farmakope Herbal Indonesia (Edisi II). In Jakarta: Departemen Kesehatan Republik Indonesia; 2012. p. 213–8.
30. Ahmed A. Al Tamimi DM. Isab AA. Alkhawajah AMM. Shawarby MA. Histological Changes in Kidney and Liver of Rats Due to Gold (III) Compound [Au(en)Cl2]Cl. PLoS One. 2012; 7(12): 1–11. https://doi.org/10.1371/journal.pone.0051889
31. Mukhtarini. Ekstraksi, Pemisahan Senyawa, dan Identifikasi Senyawa. Jurnal Pharmacy. 2014; 5: 361-367. https://dx.doi.org/10.24252/kesehatan.v7i2.55
32. Kemenkes RI. Farmakope Herbal Indonesia Edisi 2. Jakarta: Kementerian Kesehatan Republik Indonesia; 2017.
33. Arifin H. Alwi TI, Aisyahharma O. Juwita DA. Kajian Efek Analgetik dan Toksisitas Subakut dari Ekstrak Etanol Daun Kitolog (Isotoma longiflora L.) pada Mencit Putih Jantan. Jurnal Sains Farmasi Klinis. 2018; 5(2): 112–118. https://doi.org/10.25077/jsfk.5.2.112-118.2018
34. Aulya NA. Yuliawati KM. Aktivitas Antioksidan Secara Kualitatif Pada Infused Water Kulit Buah Naga Super Merah (Hylocereus costaricensis(F.A.C. Weber) Britton and Rose). Bandung Conference Series: Pharmacy. 2021; 1(1): 24–33. https://doi.org/10.29313/bcsp.v1i1.91
35. Voight, B M, Widianto. Buku Pelajaran Teknologi Farmasi Edisi V. Soendani, editor. Yogyakarta: Gadjah Mada University Press; 1994.
36. Pangestuti EK. Darmawan P. Analisis Kadar Abu dalam Tepung Terigu dengan Metode Gravimetri. Jurnal Kimia dan Rekayasa. 2021; 2(1): 16–21. https://doi.org/10.31001/jkireka.v2i1.22
37. Paneer SP. Kumari U. Raj TD. Mohamed A. Deborah GS. Subramani P. Elucidating the Effect of hesperidin on Behavioral and biochemical markers of liver and kidney function in Sprague Dawley Rats. Res J Pharm Technol. 2023; 16(8): 3749–4. http://dx.doi.org/10.52711/0974-360X.2023.00619
38. Swathi S. Phytochemical Screening and TLC Studies of Moringa oleifera Extract: Their antibacterial and anti-oxidant activities. International Journal of Current Pharmaceutical Review and Research. 2016; 8(1): 46–49.
39. Hanani E. Analisis Fitokimia. Jakara: Penerbit Buku Kedokteran EGC; 2014.
40. US Food and Drug Administration. Consideration for the Use of Histopathology and Its Associated Methodologies to Support Biomarker Qualification. United States: Department of Health and Human Services Food and Drug Administration; 2016.
41. Price SA. Wilson LM. Patofisiologi: Konsep Klinis Proses-Proses Penyakit Edisi 6, Volume 1. Jakara: EGC; 2006.
42. Bailey SA. Zidell RH. Perry RW. Relationships between organ weight and body/brain weight in the rat: what is the best analytical endpoint?. Toxicology Pathology. 2004; 32(4): 448–466. https://doi.org/10.1080/01926230490465874
43. Dewi H, Quzwain F, Wulansari N. Histology Slide Quality Comparative Study Impregnation and Embedding Using Beeswax and Paraffin. Jambi Medical and Health Sciences International Conference (JAMHEIC). 2021; 10(2): 291–298. Retrieved from https://online-journal.unja.ac.id/kedokteran/article/view/19242
44. Sugiharto. Mikroteknik. Bogor: Departemen Pendidikan dan Kebudayaan Direktorat Jendral Pendidikan Tinggi Pusat Antar Universitas Ilmu Hayati; 1989.
45. Leslie P. Gartner, Hiatt JL. Color Textbook of Histology. Third Edition. 2007.
46. Zuhair AZ., Haider HH. Amer A. Toxic effects of Cyclophosphamide on Hepatic and Kidney tissues in Albino Mice Model. Research Journal Pharmacy Technology. 2022; 15(10): 4655–4659. http://dx.doi.org/10.52711/0974-360X.2022.00781
47. Mescher Anthony. Junquiera's Basic Histology Text and Atlas twelfth edition. United States. United States: McGraw Hill; 2010.
48. Almunawati. Budiman H., Aliza D. Histopatologi Ginjal Tikus Putih (Rattus norvegicus) yang Diinjeksi Formalin. Jurna Ilmiah Mahasiswa Veteriner. 2017; 01(3): 424–431. https://doi.org/10.21157/jim%20vet..v1i3.3372
49. Suhita NLPR. Sudira IW. Winaya IBO. Histopatologi Ginjal Tikus Putih Akibat Pemberian Ekstrak Pegagan (Centella asiatica) Peroral. Buletin Veteriner Udayana. 2013; 5(1): 63–69.
50. Preethi PJ. Nephroprotective Potential of Herbal Medicines: A Review. Asian Journal Pharmacy and Technology. 2013; 3(3): 115–118.