Author(s):
Ashish Pandey, Nimisha Srivastava, Ramesh Chandra Dubey, Suneela Dhaneshwar, Alok Kumar Shukla
Email(s):
nsrivastava3@lko.amity.edu
DOI:
10.52711/0974-360X.2024.00832 
Address:
Ashish Pandey1,2, Nimisha Srivastava1*, Ramesh Chandra Dubey3, Suneela Dhaneshwar4, Alok Kumar Shukla5
1Department of Pharmaceutics, Amity Institute of Pharmacy, Lucknow, Amity University Uttar Pradesh, Sector 125, Noida, Uttar Pradesh, India.
2Department of Pharmaceutical Sciences, Gurukula Kangri (Deemed to be University), Haridwar, Uttarakhand, India.
3Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar, Uttarakhand, India.
4Department of Pharmaceutical Chemistry, Amity Institute of Pharmacy, Amity University Maharashtra, Bhatan, Post: Somathne, Panvel, Mumbai, Maharashtra, India.
5Babu Sunder Singh College of Pharmacy, Nigohan, Lucknow, Uttar Pradesh, India.
*Corresponding Author
Published In:
Volume - 17,
Issue - 11,
Year - 2024
ABSTRACT:
Aloe- emodin is an anthraquinone derivative mainly obtained from Aloe vera plant. It is reported to have several pharmacological activities including antiviral, antiproliferative, analgesic, antidiarrheal etc. Several formulations are available in market in combination with other phytoconstituents. Hence, there is a need for an analytical method for regular analysis of aloe-emodin in marketed formulations and bulk drugs. The goal of the current study was to use a UV 1800 Shimadzu double beam spectrophotometer to design and validate a straightforward, quick, accurate, and selective analytical method for estimating aloe-emodin in bulk and in marketed formulations. Using methanol as the solvent, the spectrophotometer-based detection process was carried out at ?max of 428.5 nm. The linearity, range, specificity, accuracy/recovery, precision, and robustness of the suggested approach were all confirmed. With a correlation coefficient of more than 0.999, the aloe-emodin detector response was found to be linear over the chosen concentration range (0.1-0.7µg/mL). The accuracy was between 98.78 to 100.38%, variance of standard curve line was found to be 0.052. Among the five sample preparations, the precision (R.S.D.) ranged from 0.129 to 0.325%. The limits of quantification (LOQ) and detection (LOD) were reported to be 26.518µg/mL and 0.278µg/mL, respectively. The recovery of aloe-emodin was about 98.78 to 100.38%. The data collected showed that the commercial formulations' excipients did not interfere with the procedure. As a result, it is convenient to use these formulations for routine quality control analyses of aloe-emodin in bulk medication, marketed capsule, and other formulations.
Cite this article:
Ashish Pandey, Nimisha Srivastava, Ramesh Chandra Dubey, Suneela Dhaneshwar, Alok Kumar Shukla. UV Spectrophotometeric Analytical Method Development and Validation for Aloe-Emodin Phytochemical in Bulk and Formulations. Research Journal of Pharmacy and Technology. 2024; 17(11):5439-4. doi: 10.52711/0974-360X.2024.00832
Cite(Electronic):
Ashish Pandey, Nimisha Srivastava, Ramesh Chandra Dubey, Suneela Dhaneshwar, Alok Kumar Shukla. UV Spectrophotometeric Analytical Method Development and Validation for Aloe-Emodin Phytochemical in Bulk and Formulations. Research Journal of Pharmacy and Technology. 2024; 17(11):5439-4. doi: 10.52711/0974-360X.2024.00832 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-11-40
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