Author(s):
Awik P. D. Nurhayati, Muhammad Fatoni, Yofinta I. Salsabila, Sholeh Salispriaji, Edwin Setiawan, Nurul Jadid, First A. Wati, Mardi Santoso, Shabrina S. Ghaissani
Email(s):
awiknurhayati@gmail.com
DOI:
10.52711/0974-360X.2024.00830 
Address:
Awik P. D. Nurhayati1*, Muhammad Fatoni1, Yofinta I. Salsabila1, Sholeh Salispriaji1, Edwin Setiawan1, Nurul Jadid1, First A. Wati2, Mardi Santoso3, Shabrina S. Ghaissani4
1Department of Biology, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, East Java, Indonesia.
2Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Surabaya, Ketintang, Surabaya 60231, East Java, Indonesia.
3Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Surabaya 60111, East Java, Indonesia.
4Stem Cell and Cancer Research (SCCR), Universitas Islam Sultan Agung, Semarang 50223, Central Java, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 11,
Year - 2024
ABSTRACT:
Unoptimum curing and controlling proliferation of cancer cells in breast is due to the presence of BCSCs (Breast cancer stem cells), which are associated with stemness, self-renewal, tumour initiation and metastasis. Similarly, overexpression of c-Myc (oncogenic transcription factor) in breast cancer has become potential as target of cancer therapy. Inhibition of c-Myc in cancer cells can increase the transcription factors FoxO family members including FoxO1, 3, 4 and their target genes involved in apoptosis, cell cycle arrest and autophagy. Trisindoline is an indole timer alkaloid natural compound, which is toxic to cancer cells. For this reason, we aim to decide the activity of one of derivate compound of trisindoline, namely as trisindoline 5 on BCSCs MDA-MB-231 through cytotoxicity, apoptosis, and gene expression of c-Myc and FoxO1, 3, 4. As a result, MTT assay showed trisindoline 5 can decrease the viability of BCSCs MDA-MB-231 with IC50 13.127µg/ml. Furthermore, flow cytometry analysis shown that trisindoline 5 can induce apoptosis 5.23% at concentration of 25µg/ml. Similarly, qPCR analysis showed the highest decrease in c-Myc was found in trisindoline 5 concentration of 25µg/ml with 0.0746-fold. Meanwhile, the highest increase FoxO1, 3, 4 expression was found in trisindoline 5 concentration of 25µg/ml, 20.6452-fold, 26.4709-fold, and 12.8341-fold respectively. Therefore, we conclude that trisindoline 5 concentration of 25µg/ml was able to decrease the expression of c-Myc and increase the expression of FoxO1, 3, 4 despites, it was not effective enough in reducing the population of BCSCs MDA-MB-231.
Cite this article:
Awik P. D. Nurhayati, Muhammad Fatoni, Yofinta I. Salsabila, Sholeh Salispriaji, Edwin Setiawan, Nurul Jadid, First A. Wati, Mardi Santoso, Shabrina S. Ghaissani. The Activity of Trisindoline 5 Compound againsts c-Myc and FoxO1, 3, 4 Gene Expression on MDA-MB-231 Breast Cancer Stem Cells. Research Journal of Pharmacy and Technology. 2024; 17(11):5427-4. doi: 10.52711/0974-360X.2024.00830
Cite(Electronic):
Awik P. D. Nurhayati, Muhammad Fatoni, Yofinta I. Salsabila, Sholeh Salispriaji, Edwin Setiawan, Nurul Jadid, First A. Wati, Mardi Santoso, Shabrina S. Ghaissani. The Activity of Trisindoline 5 Compound againsts c-Myc and FoxO1, 3, 4 Gene Expression on MDA-MB-231 Breast Cancer Stem Cells. Research Journal of Pharmacy and Technology. 2024; 17(11):5427-4. doi: 10.52711/0974-360X.2024.00830 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-11-38
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