ABSTRACT:
Hardwickia binata Roxbs. has historically been used by indigenous cultures for the therapeutic management of various medical conditions. The leaves of the plant were successively extracted with ethylacetate, chloroform, acetone, and ethanol by soxhlet extraction. We assessed the ethyl acetate, chloroform, acetone, and ethanolic extracts for their antioxidant activity utilizing in vitro models, namely the DPPH radical scavenging assay, nitric acid scavenging, reducing power assay method, and the antibacterial properties of Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae were examined using the agar disc diffusion technique. The ethanolic extract was found to possess excellent antioxidant and antimicrobial activity as compared to other solvent extract. A preliminary analysis of phytochemicals was conducted, leading to the identification of several phytoconstituents within the extract as flavonoids, phenolic compounds, tannins, glycosides, and proteins. The antioxidant activity may be attributed to flavonoids and phenolics present in the drug.
Cite this article:
Snehal Praful Shingade, Rajendra Baliram Kakde. Qualitative and Quantitative Phytochemical Evaluation, Antioxidant and Antimicrobial activity of Hardwickia binata Roxbs. leaf extract in various solvent. Research Journal of Pharmacy and Technology. 2024; 17(11):5267-3. doi: 10.52711/0974-360X.2024.00806
Cite(Electronic):
Snehal Praful Shingade, Rajendra Baliram Kakde. Qualitative and Quantitative Phytochemical Evaluation, Antioxidant and Antimicrobial activity of Hardwickia binata Roxbs. leaf extract in various solvent. Research Journal of Pharmacy and Technology. 2024; 17(11):5267-3. doi: 10.52711/0974-360X.2024.00806 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-11-14
REFERENCES:
1. Halliwell B, Gutteridge JM,Oxygen toxicity, oxygen radicals, transition metals and disease. Biochemical Journal. 1984; 219(1): 1–4. doi: 10.1042/bj2190001
2. Jin D, Hakamata H, Takahashi K, Kotani A, Kusu F, Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection, Biomedical Chromatography. 2004; 18(9): 662-666. DOI: 10.1002/bmc.370
3. Wongkham S, Laupattarakasaem P, Pienthaweechai K, Areejitranusorn P, Wongkham C, Techanitiswad T, Antimicrobial activity of Streblusasper leaf extract. Phytotherapy Research. 2001; 15(2): 119–121. Doi: 10.1002/ptr.705.
4. Duh PD, Antioxidant activity of burdock (ArctiumlappaLinne): its scavenging effect on free radical and active oxygen. Journal of the American Oil Chemist’s Society. 1998; 75(4): 455– 465.
5. Duh PD, Tu YY, Yen GC, Antioxidant activity of water extract of HarngJyur (Chrysanthemum morifolium Ramat). Lebnesmittel-Wissenschaft Food Science and Technologies. 1999; 32(5): 269–277.
6. Ito N, Fukushima S, HagiwaraA, Shibata M, Ogiso T,Carcinogenicity of buthylatedhydroxyanisole in F344 rats. Journal of the National Cancer Institute. 1983; 70(2): 343–352.
7. Zheng W, Wang SY, Antioxidant activity and phenolic compounds in selected herbs. Journal of Agricultural and Food Chemistry. 2001; 49(11): 5165–5170.DOI: 10.1021/jf010697n
8. Kinsella JE, Frankel E, German B, Kanner J, Possible mechanism for the protective role of the antioxidant in wine and plant foods. Food Technology. 1993; 47: 85–89.
9. Rajendran R, Hemachander R, Ezhilarasan T, Keerthana C, Saroja DL, Saichand KV and Mohamed Gasim Abdullah, Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica LinLeaves. Research J. Pharm. and Tech. 2010; 3(2) 551-555.
10. Rice-Evans C, Miller N, Paganga G, Antioxidant properties of phenolic compounds. Trends. Plant Sci. 1997; 2(4): 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
11. Velioglu YS, Mazza G, Gao L, Oomah, BD, Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. Journal of Agricultural and Food Chemistry. 1998; 46: 4113–4117.http://dx.doi.org/10.1021/jf9801973
12. Decker E A, Phenolics: prooxidants or antioxidants? Nutrition Reviews. 1997; 55: 396–407. DOI: 10.1111/j.1753-4887.1997.tb01580.x
13. Cook NC, Samman S, Flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources. Journal of Nutritional Biochemistry. 1996; 7: 66–76. https://doi.org/10.1016/0955-2863(95)00168-9
14. Pietta PG, Flavonoids as antioxidants. Journal of Natural Product. 2000; 63(7): 1035-42. doi: 10.1021/np9904509.
15. Sahu S, Green S, Food antioxidants: Their dual role in carcinogenesis. 1997. DOI: 10.1201/9780203744673-19
16. Sanchez-Moreno C, Larrauri JA, Saura-Calixto FA, Procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture. 1998; 76: 270–276. doi:10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9
17. Campos FM, Couto JA, Hogg TA, Influence of phenolic acids on growth and inactivation of Oenococcusoeni and Lactobacillus hilgardii. Journal of Applied Microbiology. 2003; 94(2): 167–174. doi: 10.1046/j.1365-2672.2003.01801.x.
18. Tiwari P, Patel RK, Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods. Asian J. Pharm. Ana. 2013; 3(4): 147-152
19. Seetharam YN, Kotresaha K,Rao S,Medicinal Plants in and around Gulbarga University, Gulbarga. The Swamy Botany Cl. 1993; 10(1 and 2): 31-35.
20. Seetharaam YN, Haleshi CV, Medicinal plants of north-eastern Karnataka and their status. My Forest. 1998; 34(4): 767-72.
21. AlexiadesMN, LacazeD, Medicinal Resources of the Tropical Forest. Columbia University Press, New York,1994; 341–365
22. Khare CP, Indian Medicinal Plants: An Illustrated Dictionary. Springer Science and Business Media. 2008; 302.
23. Salai SMS, Vaidyanathan D, Sisubalan N, Medicinal plants using traditional healers and Malayali tribes in Jawadhu hills of Eastern Ghats, Tamil Nadu, India. Advances in Applied Science Research. 2014; 5(2): 292-304.
24. Vaidyanathan D, Sisubalan N, Ghouse BM, Survey of ethanomedicinal plants and folklore studies on Malayali Tribate of Vellakadai village a part of Shrervaroy R. International Journal of Recent Scientific Research. 2014; 5(7): 1368-80.
25. Pankaj O, Medicinal herbs of Chhattisgarh. India Having Less Traditional us. 2003.
26. Gunaselvi G,Kulasekaren V, GopalV, Anti-bacterial and anti-fungal activity of various leaves extracts of Hardwickia binata roxbs, International Journal of Pharm Tech Research. 2010; 2(4): 2183-2187.
27. Sharanabasappa G, Mallikharjuna PB, Rajanna LN, Seetharam YN, Antibacterial and Analgesic activities of BauhiaAcemosa Lam and Hardwickia binata Roxbs leaf extracts. Pharmacology Online. 22010; 557-560.
28. KaramanY, Sahin F, Gulluce M, Ogutcu H, Sengul M, Adıguzel, A, Antimicrobial activity of aqueous and methanol extracts of Juniperusoxycedrus L. Journal of Ethnopharmacology. 2003; 85(2-3): 231–235. DOI: 10.1016/s0378-8741(03)00006-0.
29. Harborne JB, Phytochemical methods, a guide to modern techniques of plant analysis. Chapman and Hall, London 1973; 267–270.
30. Hagerman AE, Riedl KM, Jones GA, Sovik KN, Ritchard NT, Hartzfeld PW, High molecular Weight plants polyphenolics (tannins) as biological antioxidants, Journal of Agriculture and Food Chemistry. 1998; 46(5): 1887-1892. DOI: 10.1021/jf970975b
31. Tambe VD, Bhamber RS, Estimation of phenol, tannins alkaloid and flavonoid in Hibiscustiliaceuslinn. Wood Extract. 2014; 2(4): 41-47.
32. Rodrigo S, Helena TG, Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method. Food Chemistry. 2009; 112(3): 654-658.
33. Garrat DC, The quantitative analysis of drug1964;456-458.
34. Gunasekaran B, Muralidharan P, Pandiselvi A, Amutha P, Preliminary Phytochemical screening and Anti-oxidant activities of Ethanolic extract of Caesalpiniasappan Linn,. Research J. Pharm. and Tech. 2008;1(3): 179-181
35. Oyaizu M, Studies on Products of Browning Reactions: Antioxidative Activities of Product of Browning Reaction Prepared from Glucosamine. Japan Journal of Nutrition. 1986; 44: 307–315.http://dx.doi.org/10.5264/eiyogakuzashi.44.307
36. Indian Pharmacopeia, Government of India, Ministry of Health and Family Welfare, Published by the Controller of Publications Delhi, 1996 II; 218.
37. Felhi S, Daoud A, Hajlaoui H, Mnafgui K, Gharsallah N, Kadri A, Solvent extraction effects on phytochemical constituents profiles, antioxidant and antimicrobial activities and functional group analysis of Ecballium elaterium seeds and peels fruits. Food Sci Techno. 2017; 37 (3): 483–492. https://doi.org/10.1590/1678-457X.23516.
38. Saebi A, Minaei S, Mahdavian AR, Ebadi MT, Precision harvesting of medicinal plants: elements and ash content of hyssop (Hyssopusofficinalis L.) as affected by harvest height. Biol Trace Elem Res. 2021; 199(2): 753–762. DOI: 10.1007/s12011-020-02171-2
39. Chanda S, Importance of pharmacognostic study of medicinal plants: an overview. Journal of Pharmacognosy and Phytochemistry. 2014; 2 (5): 69–73.
40. Namrta C, Siddiqui MB, Sayyada K, Pharmacognostic evaluation of Tinosporacordifolia (Wild.) Miers and identification of biomarkers. Indian J Tradit Knowl. 2014; 13(3): 543–550.
41. Diniz TC, Silva JC, de Lima-Saraiva SR, Ribeiro FP, Pacheco AG, de Freitas RM, Quintans-Júnior LJ, Quintans J, Mendes RL, Almeida JR, The role of flavonoids on oxidative stress in epilepsy. Oxid Med Cell Longev. 2015;https:// doi. org/ 10. 1155/ 2015/ 171756
42. Soares JR, Dinis TCP, Cunha AP, Almeida LM, Antioxidant activities of some extracts of Thymus zygis. Free Radic Res 1997; 26(5): 469–478. DOI: 10.3109/10715769709084484
43. Gould IM, Bal AM, New antibiotic agents in the pipeline and how they can help overcome microbial resistance. Virulence. 2013; 4(2): 185–191. https:// doi. org/ 10. 4161/ VIRU. 22507
44. Michael CA, Dominey-Howes D, Labbate M The antimicrobial resistance crisis: causes, consequences, and management. Front Public Health. 2014; 2:145. https:// doi. org/ 10. 3389/ FPUBH. 2014. 00145.
45. Sonia R, savithri S, Sangeetha R, Shridevi G, In-vitro Antimicrobial activity of Mtad Photoactivated Disinfection along with Chlorohexidine on Enterococcus feacalis, Staphylococcus aureus and candida albicans, Research Journal of Pharmacy and Techanology. 2024; 17(8): 3752-6. DOI: 10.52711/0974-360X.2024.00583