Author(s):
Hanafi, Sri Redjeki Setyawati, Nurdiani, Adya Rizky Pradipta, Alvina Nur Aini, Septilina Melati Sirait
Email(s):
septilinamelati.aka@gmail.com
DOI:
10.52711/0974-360X.2024.00736
Address:
Hanafi1, Sri Redjeki Setyawati2, Nurdiani3, Adya Rizky Pradipta4, Alvina Nur Aini5, Septilina Melati Sirait6*
1,2,5,6Department of Food Industry Quality Insurance, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
3Department of Industrial Waste Treatment, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
4Department of Chemistry Analysis, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
*Corresponding Author
Published In:
Volume - 17,
Issue - 10,
Year - 2024
ABSTRACT:
The food and beverage industry often uses synthetic dyes to restore the color of food products that fade during the production process. One natural dye that can replace synthetic dyes is derived from carotenoid compounds. Apart from giving food a yellow, orange or red color, carotenoids also have an antioxidant function that can protect the body from free radicals. The many functions of carotenoids for health mean that carotenoids can also be applied to products in the form of carotenoid powder. Carotenoid pigments can be taken from the source, namely by extracting them. There are several extraction methods ranging from conventional to modern methods. Using the enzymatic carotenoid extraction method has advantages compared to other methods, namely that it does not use a lot of solvent, obtains a high extraction yield, and is environmentally friendly due to low energy consumption. Carrotenoid extraction tool can be operated to extract carotenoids from carrots with a capacity of 2.5 liters of carrot pulp. Enzyme activity test showed that the hydrolysis time was 135 minutes, pH 5, temperature 50oC and the addition of enzyme pectinase 2% and cellulase 2% was the optimum treatment with the highest reducing sugar content of 37.60mg/mL. The highest levels of carotenoids were obtained from maceration extraction with a methyl acetate solvent of 10.49%, extraction using a device with a methyl acetate solvent of 25.73%, and extraction with a tool and the enzyme methyl acetate solvent was obtained 62.00%.
Cite this article:
Hanafi, Sri Redjeki Setyawati, Nurdiani, Adya Rizky Pradipta, Alvina Nur Aini, Septilina Melati Sirait. Design and Development of A Semy Continuous Carotenoid Tool with Enzym and Environmental Friendly Solution. Research Journal of Pharmacy and Technology. 2024; 17(10):4780-4. doi: 10.52711/0974-360X.2024.00736
Cite(Electronic):
Hanafi, Sri Redjeki Setyawati, Nurdiani, Adya Rizky Pradipta, Alvina Nur Aini, Septilina Melati Sirait. Design and Development of A Semy Continuous Carotenoid Tool with Enzym and Environmental Friendly Solution. Research Journal of Pharmacy and Technology. 2024; 17(10):4780-4. doi: 10.52711/0974-360X.2024.00736 Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-10-21
REFERENCES:
1. Ibtihal Q A. Hala I I. Hayfa M J. Adil H D. Synthesis and Identification of new compounds have Antioxidant activity Beta-carotene, from Natural Auxin Phenyl Acetic Acid. Research J. Pharm. and Tech. 2020; 13(1): 40-46. https://doi.org/10.5958/0974-360X.2020.00007.4
2. Amaya D B R. Natural Food Pigments and Colorants. Current Opinion in Food Science. 2016; 7: 20–26. https://doi.org/10.1016/j.cofs.2015.08.004
3. Amita G. Ajita D. Ajay S. Kamlesh M. Extraction of Beta Carotene from Selected Dried and Fresh Samples of Vegetables. Asian J. Research Chem. 2013; 6(2): 169-171
4. Kim S M. Jung Y J. Kwon O N. Cha K H. Um B H. Chung D. Pan C H. Potential Commercial Source of Fucoxanthin Extracted from The Microalga Phaeodactylum tricornutum. Applied Biochemistry and Biotechnology. 2012; 166(7): 1843 – 1855. https://doi.org/10.1007/s12010-012-9602-2
5. Hala J. Fida A. Effect of Boiling on Carotenoids content of Pumpkin available in Damascus, Syria. Research Journal of Pharmacy and Technology. 2021; 14(11): 5642-6. https://doi.org/10.52711/0974-360X.2021.00981
6. Sachin A. Amol G. Prasad M. Prashant S. Pramod G. Pratik K. Shubham K. Yogesh G. Different methods of extraction for Red Dye from Capsicum annuum. Research J. Science and Tech. 2019; 11(4): 281-286. https://doi.org/10.5958/2349-2988.2019.00040.8
7. Amit T. Dinesh S. Rajendra K. The Chemistry and Biology of Bioflavonoids. Research J. Pharm. and Tech. 2008; 1(3): 132-143.
8. Seema R. Nikam. Amol S. Jagdale. Sahebrao S. Boraste. Shrikant B. Patil. Bioanalysis - Method Development, Validation, Sample Preparation, its Detection Techniques and its Application. Asian Journal of Pharmaceutical Analysis. 2021; 11(4): 297-5. https://doi.org/10.52711/2231-5675.2021.00051
9. Miller G L.Use of Dinitrosalicile Acid Reagent for Determination of Reducing Sugar. J Anal Chem. 1959; 31(3): 426-428. https://doi.org/10.1021/ac60147a030
10. Cinar I. Storage Stability of Enzyme Extracted Carotenoids Pigments from Carrots. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2004; 3(1): 609-616
11. Vijay K P. Chirag K. Harsha U P. Patel C N. Vitamins, Minerals and Carotenoids as a Antioxidants. Asian J. Research Chem. 2010; 3(2): 255-260.
12. Archana R. P. Dattaprasad N. Vikhe R.S. Jadhav. Recent Advances in Extraction Techniques of Herbals – A Review. Asian J. Res. Pharm. Sci. 2020; 10(4): 287-292. https://doi.org/10.5958/2231-5659.2020.00050.8
13. Arif J K. Abdi D. Armaini. Syafrizayanti. Zulkarnain C. Carotenoid Profile of Freshwater Microalgae Mychonastes racemosus AUP1 and its Antioxidant properties. Research Journal of Pharmacy and Technology. 2023; 16(1): 404-410. https://doi.org/10.52711/0974-360X.2023.00069
14. Perumal A. Tamilselvy S, Indra P. Green Synthesis of Silver Nanoparticles from Carrot. Research J. Pharm. and Tech 2018; 11(7): 2757-2760. https://doi.org/10.5958/0974-360X.2018.00509.7