Author(s): Bunga Nur Annisa, Vriezka Mierza, Sofa Fajriah, Rizna Triana Dewi, Marissa Angelina, Gian Primahana, Sukirno

Email(s): nurannisabunga@gmail.com , sofafajriah@gmail.com

DOI: 10.52711/0974-360X.2024.00009   

Address: Bunga Nur Annisa1*, Vriezka Mierza1, Sofa Fajriah2*, Rizna Triana Dewi2, Marissa Angelina2, Gian Primahana2, Sukirno2
1Department of Pharmacy, Faculty of Health Sciences, Singaperbangsa University Karawang, Jl. HS. Ronggo Waluyo, West Java, Indonesia.
2National Research and Innovation Agency, KST Habibie Serpong, Banten, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
Centella asiatica (L.) Urban is known to have a pharmacological effect as an antioxidant. Indonesian research on gotu kola, especially the ethyl acetate fraction it produces, is rarely carried out. Previous research stated that kaempferol and quercetin which isolated from the ethyl acetate fraction of Centella asiatica herb had antioxidant activity with respective IC50 values of 16.50 and 21.61 µg/mL, so this study aimed to get other compounds from the ethyl acetate fraction which has antioxidant activity. The purposes of this study were to isolate, identify, and examine the free radical scavenging activity of DPPH from the ethyl acetate fraction of gotu kola (C.asiatica (L.) Urban) herb. Isolation of the ethyl acetate fraction was carried out from 70% ethanol extract of Centella asiatica plant using vacuum liquid chromatography and gravity column chromatography afforded a new asiatic acid derivative. Elucidation of a new compound based on UV-Vis, FT-IR, LC-MS/MS, 1D-NMR and 2D-NMR to get 11-hydroxy-9-(hydroxymethyl)-1,2,6a,6b,9,12a-hexamethyl-10-((3,4,5-trihydroxytetrahydrofuran-2-yl)oxy )-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid and it had moderate antioxidant activity with IC50 of 106.99 ± 1.76 µg/mL. The pure isolate obtained is suspected to be a new compound derived from asiatic acid.


Cite this article:
Bunga Nur Annisa, Vriezka Mierza, Sofa Fajriah, Rizna Triana Dewi, Marissa Angelina, Gian Primahana, Sukirno. Research Journal of Pharmacy and Technology. 2024; 17(1):51-8. doi: 10.52711/0974-360X.2024.00009

Cite(Electronic):
Bunga Nur Annisa, Vriezka Mierza, Sofa Fajriah, Rizna Triana Dewi, Marissa Angelina, Gian Primahana, Sukirno. Research Journal of Pharmacy and Technology. 2024; 17(1):51-8. doi: 10.52711/0974-360X.2024.00009   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-9


REFERENCES:
1.    Annisa BN, Tama AP, Sa’adah CN, Sary NV. Flavonoid Isolation Methods in Plants in Indonesia. Pharmacine. 2021; 2(1): 22–35. ISSN 2746-4199
2.    Yahya MA, Nurrosyidah IH. Antioxidant Activity of Gotu Kola (Centella asiatica (L.) Urban) Herbal Extract Using DPPH (2,2-Diphenyl-1-Pikrylhidrazil) Method. J Halal Prod Res. 2020; 3(2): 106–12. https://dx.doi.org/ 10.20473/jhpr.vol.3-issue.2.106-112
3.    Sadik F, Anwar ARA. Standardization of Specific Parameters of Ethanol Extract of Centella asiatica L. Leaves as Antidiabetic. J Syifa Sci Clin Res. 2022; 4(1): 1–9. https://doi.org/10.37311/jsscr.v4i1.13310
4.    Widyani M, Ulfa M, Wirasisya DG. Free Radical Inhibition Effect of Infusion and Ethanol Extract of Centella Asiatica (L.) Urb with DPPH Method. J Pijar MIPA. 2019; 14(2132): 100–6. doi.org/10.29303/jpm.v14.i1.1006
5.    Widuri SA, Mediawati I. Phytochemical Screening and Antioxidant Activity Tests of Several Medicinal Plants in Paser District, East Kalimantan. Pros Semin Nas Lingkung Lahan Basah. 2018; 3(1): 116–20.
6.    Manikandan R, Anand AV. A Review on Antioxidant activity of Psidium guajava. Res J Pharm Tech. 2015; 8(3): 339–42. doi.org/10.5958/0974-360X.2015.00056.6
7.    Arnanda QP, Nuwarda RF. The Use of Technicium-99M Radiopharmaceuticals From Glutathione Compounds and Flavonoid Compounds For Early Detection Of Cancer-Triggering Free Radicals. Farmaka Suplemen. 2019; 14(1): 1–15. ISBN 8995899250143
8.    Salmiwanti, Ilyas A, Saleh A. Isolation of Secondary Metabolite Compounds N-Hexanes Fraction and Antibacterial Tests against Mycobacterium Tuberculosis. Al-Kimia. 2012; 4(2): 54–63. https://doi.org/10.24252/al-kimia.v4i2.1679
9.    Dewi R, Maryani F. Antioxidant and α-Glucosidase Inhibitory Compounds of Centella Asiatica. Procedia Chem [Internet]. 2015; 17: 147–52. Available from: http://dx.doi.org/10.1016/j.proche.2015.12.130
10.    Sutardi S. The content of the active ingredients of Centella asiatica and its properties to enhance the body's immune system. J Penelit dan Pengemb Pertan. 2017; 35(3): 121. doi.org/10.21082/jp3.v35n3.2016.p121-130
11.    Mudjihartini N, Paramita R, Prijanti AR, Sarsanti PAN, Fadilah F, Purwaningsih EH. The effects of Centella asiatica and Acalypha indica l. Extracts on aging process. Res J Pharm Technol. 2020; 13(2): 787–90. https://doi.org/10.32889/actabioina.79
12.    Kangralkar V., Kulkarni A. Evaluation of effect of Piper betel, Centella asiatica and Aristolochia indica extracts on bacterial enzymes in 1, 2-dimethyl hydrazine induced colon cancer in wistar rats. Res J Pharm Tech. 2014; 7(2): 151–4.
13.    Swastini DA, Udayana INK, Arisanti CIS. Cold cream combination of Garcinia mangostana L. Anredera cordifolia (Ten.) and Centella asiatica extracts on Burn Healing Activity Test. Res J Pharm Tech. 2021; 14(5): 2483–6. doi.org/10.52711/0974-360X.2021.00437
14.    Raju DC, Varghese RJ, Biji N. In vitro production of neurologically active phytoconstituents from an elite chemovariant of centella asiatica (L.), identified from south Indian populations. Res J Pharm Technol. 2017; 10(7): 2095–100. doi.org/10.52711/0974-360X.2023.00068
15.    Yadav AV, Upasani CD. Protective effect of the combination of Hydroalcoholic extracts of Asparagus Racemosus, Centella Asiatica, Plumeria rubra with Glibenclamide in Experimentally Induced Diabetic Nephropathy in rats. Res J Pharm Tech. 2022; 15(4): 1641–0. doi.org/ 10.52711/0974-360X.2022.00270
16.    Rachmatiah T, Putri F., Dewi R. Activity of Ethanol and Methanol Extracts of Centella Asiatica (L.) Urban. var. Manoko Leaves as Antioxidant and Antidiabetic In Vitro. Sainstech Farma. 2015; 8(2): 14–7. https://doi.org/10.37277/sfj.v8i2.382
17.    Mareta CA. The Effectiveness of Gotu Kola (Centella Asiatica) as an Antioxidant. J Med Hutama [Internet]. 2020; 2(1): 390–4. Available from: http://jurnalmedikahutama.com.
18.    Raju DC, Victoria TD, Biji N, Nikitha G. Evaluation of Antioxidant Potential of Ethanolic Extract of Centella asiatica L. Res J Pharm Technol. 2015; 8(9): 1289–93. doi.org/ 10.5958/0974-360X.2015.00234.6
19.    Salamah N, Farahana L. Antioxidant Activity Test of Centella asiatica (L.) Urb Herba Centella asiatica Extract with Phosphomolybdate Method. Pharmaciana. 2014; 4(1): 23–9. doi.org/10.12928/pharmaciana.v4i1.394
20.    Howan DHO. Isolation and Identification of Secondary Metabolites from Butanol Extract of Gotu Kola (Centella asiatica (L) urban). Fuller J Chem. 2017; 2(2): 92. doi.org/10.37033/fjc.v2i2.16
21.    Nema NK, Maity N, Sarkar BK, Mukherjee PK. Matrix Metalloproteinase, Hyaluronidase and Elastase Inhibitory Potential of Standardized Extract of Centella asiatica. Pharm Biol. 2013; 51(9): 1182–7. https://doi.org/10.3109/13880209.2013.782505
22.    Fajriah S, Megawati M. Phytochemical Screening and Toxicity Test of Myristica fatua Houtt Leaves. Chim Nat Acta. 2015; 3(3): 116–9. doi.org/10.24198/cna.v3.n3.9219
23.    Fadhli H, Lukman A, Adawiyah R. Isolation and Antioxidant Activity Test of Secondary Metabolites from Ethyl Acetate Extract of Kangkang Valve Bark (Bauhinia semibifida Roxb). Al-Kimia. 2020; 8(1): 36–45. doi.org/10.24252/al-kimia.v8i1.10152
24.    Amaliah N, Salempa P, Muharram M. Isolation and Identification of Secondary Metabolite Compounds from the Methanol Fraction of the Milk Grassroots (Scindapsus pictus Hassk.). Chem J Ilm Kim dan Pendidik Kim. 2020; 21(1): 78–85. doi.org/10.35580/chemica.v21i1.14841
25.    Krishnan V, Singh S, Kaur C, Dahuja A, Praveen S. Anthocyanin A premium functional superfood supplement. New Delhi: ICAR-INDIAN AGRICULTURAL RESEARCH INSITITUTE; 2017.
26.    Tunnisa F, Nur Faridah D, Afriyanti A, Rosalina D, Ana Syabana M, Darmawan N, et al. Antioxidant and Antidiabetic Compounds Identification in Several Indonesian Underutilized Zingiberaceae Spices Using SPME-GC/MS-Based Bolatilomics and In Silico Methods. Food Chem X [Internet]. 2022; 14(November 2021):1–10. Available from: https://doi.org/10.1016/j.fochx.2022.100285
27.    Cahyono B, Prihatini CS, Suzery M, Bima DN. Determination of Antioxidant Activity of Quercetin Compounds and Galangal Extract Using HPLC and UV-Vis. Alchemy. 2021; 8(2): 24–32. doi.org/10.18860/al.v8i2.10594
28.    Maesaroh K, Kurnia D, Al Anshori J. Comparison of DPPH, FRAP and FIC Antioxidant Activity Test Methods Against Ascorbic Acid, Gallic Acid and Quercetin. Chim Nat Acta. 2018;6(2):93–100. https://doi.org/10.24198/cna.v6.n2.19049
29.    Musfiroh I, Nursyamsiah T, Sutrisna E, Muhtadi A, Kartasasmita RE, Ibrahim S. Isolation and Characterization of Asiatic Acid from Ethanol Extract of Gotu Kola (Centella Asiatica. (L.) Urban). J Farm Indones. 2015; 7(4): 203–9.
30.    Annadurai P. Extraction and Isolation of Bioactive Compounds from Lantana camara Leaves by Column Chromatographic Techniques. Res J Pharm Tech. 2021; 14(3): 1607–11. doi.org/10.5958/0974-360X.2021.00285.7
31.    Ambarwati N, Rakhmawati R, Sari D, Wahyuni C. Toxicity Test of Ambre Leaf Fraction (Geranium radula) Against Artemia salina and Chemical Content Profile of the Most Active Fraction. Biofarmasi. 2015;13(1):15–24. doi.org/10.13057/biofar/f130103
32.    Jasiem TM. Isolation of Terpene and Pharmacognostical Study of Iraqi Caper Shrubs (Capparis spinosa ) L. Res J Pharm Tech. 2018; 11(6): 2388–92. doi.org/10.5958/0974-360X.2018.00441.9
33.    Dachriyanus. Structural Analysis of Organic Compounds by Spectroscopy. Padang: Lembaga Pengembangan Teknologi Informasi dan Komunikasi (LPTIK); 2017.
34.    He WN, Dai JG, Ye M, Wu LJ, Guo DA. Microbial Transformation of Asiatic Acid by Alternaria Longipes. J Asian Nat Prod Res. 2014; 12(9): 760–4. doi.org/10.1080/10286020.2010.501505
35.    Sastrohamidjojo H. Spectroscopy Fundamentals. Yogyakarta: Gadjah Mada University Press; 2013.

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