Author(s): Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari

Email(s): imaskhairani@gmail.com

DOI: 10.52711/0974-360X.2024.00061   

Address: Ardina Purnama Tirta1, Imas Solihat2*, Achmad Nandang Roziafanto2, Nurhasanah3, Ratnawati L Djanis4, Inda Mapiliandari1
1Department of Chemical Analysis, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
2Department of Food Nanotechnology, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
3Department of Quality Assurance of Food Industry, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
4Department of Industrial Waste Treatment, Politeknik AKA Bogor, Jalan Pangeran Sogiri, Tanah Baru, Bogor 16154, Indonesia.
*Corresponding Author

Published In:   Volume - 17,      Issue - 1,     Year - 2024


ABSTRACT:
A study has been conducted on the phytochemical profile and pharmacological activity of Gedi (Abelmoschus manihot (L.) Medik) using a multistage maceration method. The results of the phytochemical screening test showed that the crude extract of Gedi leaves gave a positive reaction to the presence of alkaloids, tannins, phenols, flavonoids, unsaturated steroids and saponins. The total phenolic analysis showed that the total phenolic concentrations of Gedi leaf extracts in methanol, ethyl acetate, and hexane extracts were 1.70, 1.44, and 0.85mg GAE/g extract, respectively. The results of the antioxidant activity assays obtained an IC50 value for methanol, ethyl acetate, and hexane extracts of Gedi leaves, which were 40.77, 155.50, and 25.84mg/L, respectively. The antimicrobial activity results showed that all three Gedi leaf extracts had activity in inhibiting microbial growth. The inhibitory values for methanol, ethyl acetate, and hexane extracts of Gedi leaves were 7.0, 13.5, and 9.5mm, respectively, for Escherichia coli, and 21.5, 15.0, and 13.0mm, respectively, for Bacillus. The toxicity test results showed that the LC50 values of Gedi leaf extracts for methanol, ethyl acetate, and hexane extracts were 500 ppm, 736ppm, and 2249ppm, respectively.


Cite this article:
Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari. Multi-Step Maceration Extraction and Pharmacological Evaluation of Abelmoschus manihot (L.) Medik. Research Journal of Pharmacy and Technology. 2024; 17(1):389-5. doi: 10.52711/0974-360X.2024.00061

Cite(Electronic):
Ardina Purnama Tirta, Imas Solihat, Achmad Nandang Roziafanto, Nurhasanah, Ratnawati L Djanis, Inda Mapiliandari. Multi-Step Maceration Extraction and Pharmacological Evaluation of Abelmoschus manihot (L.) Medik. Research Journal of Pharmacy and Technology. 2024; 17(1):389-5. doi: 10.52711/0974-360X.2024.00061   Available on: https://rjptonline.org/AbstractView.aspx?PID=2024-17-1-61


REFERENCE:
1.    Poongothai Annadurai. Extraction and Isolation of Bioactive Compounds from Lantana camara Leaves by Column Chromatographic Techniques. Research J. Pharm. and Tech. 2021; 14(3): 1607-1611. doi: 10.5958/0974-360X.2021.00285.7 Available on: https://rjptonline.org/AbstractView.aspx?PID=2021-14-3-74
2.    Gani N, Momuat LI, Pitoi M. Profil lipida plasma tikus wistar yang hiperkolesterolemia pada pemberian Gedi merah (Abelmoschus manihot  L.). Jurnal MIPA Unsrat online. 2013.
3.    Mamahit LP, Soekamto NH. Satu senyawa asam organic yang diisolasi dari daun Gedi (Abelmoschus manihot  L. Medik) asal Sulawesi Utara. Chem.Prog. 2010;  3(1): 42.
4.    Hodgson JM and Kevin DC. Review dietary flavonoids:effect on endothelial function and blood pressure. J Sci Food Agric. 2006; 86: 2492-2498
5.    Zhao CQ, Zhou Y, Ping J, Xu LM. Traditional chinese medicine for treatmentof liver disease:progress, challenges and opportunities. J Integr Med. 2014; 12(5):401-408
6.    Tu Y, Sun W, Wan YG, Che XY, Pu Hp, Yin XJ, Chen HL, Meng Xj, Huang YR, Shi XM. Huangkui capsule, an extract from Abelmoschus manihot  (L) medic, ameliorates Adriamycin-induced renal inflammation and glomerular injuryvia inhibiting p38MAPK signaling pathway activity in rats. J Entnopharmacol. 2013; 147(2):311-320.
7.    Pine ATD, Alam G and Attamim F. Standarisasi mutu ekstrak daun Gedi (Abelmoschus manihot  L.Medik) dan uji efek antioksidan dengan metode DPPH. 2011; 113: 125
8.    Jain, P and Bari, S. Antimicrobial properties of Abelmoschus manihot Linn. Internationale Pharmaceutica Sciencia. 2011; 1(2):32-35.
9.    Jain, P.S., A.A. Todarwal., S.B. Bari., and S.J. Sanjay. Analgesic activity of Abelmoschus manihot extracts. International J. of Pharmacology. 2011; 7: 716-720.
10.    Guo, J. C., Xue, J.A., Duan, D., Qian, Y., Tangand You, Y. Anticonvulsant, antidepressant-like activity of Abelmoschus manihot ethanol extract and its potential active components in vivo. Phytomedicine: International J. of Phytotherapy & Phytopharmacology.  2011; 3:132 – 136
11.    Guo, J.M., P. Ling., Y.W. Lu., J.A. Duan., E.X. Shang., D.W. Qian., and Y.P. Tang. Investigation of in vivo metabolic profile of Abelmoschus manihot based on pattern recognition analysis. J. of Ethnopharmacology. 2013; 04: 29
12.    Ai G, Liu Q, Hua W, Huang Z, Wang D. Hepatoprotective evaluation of the total flavonoids extracted from flowers of Abelmoschus manihot  (L) Medik:in vitro and vivo studies. J Enthopharmacol. 2013;146 (3):794-802
13.    Manish Devgun, Arun Nanda, SH Ansari, SK Swamy. Recent Techniques for Extraction of Natural Products. Research J. Pharm. and Tech. 2010; 3(3): 644-649.   Available on: https://rjptonline.org/AbstractView.aspx?PID=2010-3-3-23
14.    Elena-Emilia Oprescu, Cristina-Emanuela Enascuta, Elena Radu, Mihaela Ciltea-Udrescu, Vasile Lavric, Does the ultrasonic field improve the extraction productivity compared to classical methods – Maceration and reflux distillation?, Chemical Engineering and Processing - Process Intensification. 2022; 179: 109082  https://doi.org/10.1016/j.cep.2022.109082.
15.    V. Vyshali, N. Lalitha Jyotsna, Vasudha Bakshi, Syed Ismail, Dibyalochan Mohanty, T. Kavitha, M. Manoj. Optimization of Extraction of Curcuminoids from Turmeric Powder (Curcuma longa). Research J. Pharm. and Tech. 2021; 14(9): 4615-0. doi: 10.52711/0974-360X.2021.00802
16.    Brecht Dewulf, Vincent Cool, Zheng Li, Koen Binnemans,Effect of polar molecular organic solvents on non-aqueous solvent extraction of rare-earth elements,Separation and Purification Technology. 2022; 294:121197 https://doi.org/10.1016/j.seppur.2022.121197.
17.    Shmygareva Anna Anatolyevna, Kurkin Vladimir Alexandrovich, Sankov Anatoliy Nikolayevich, Rybalko Maya Victorovna, Semeniuta Ksenia Nikolaevna. Method of obtaining of extract by the method of Modified Maceration. Research J. Pharm. and Tech. 2019; 12(12): 5956-5958. doi: 10.5958/0974-360X.2019.01033.3
18.    Spigno G, Tramelli L and de Faveri DM. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics. Journal of Food Engineering. 2010; 81:200-208
19.    Hossain MA, Shah MD, Gnanaraj C and Iqbal M. In vitro total phenolics, flavonoid content and antioxidant activity of essensial oil, various organis extracts from the leaves of tropical plant tetrastigma from sabah. Asian Pacific Journal of Tropical Medicine. 2011; 4(9):717-721
20.    Sivanandham V. 2015. Phytochemical techniques-a review. World journal of science and research. 1(2):80-91.
21.    Hung CY and Yen GC. Antioxidant of phenolic compounds isolated from Mesona procumbens hemsil. Journal of Agricultural and Food Chemistry. 2002; 50: 2993-2997
22.    Dhinakaran, Isaac D and Geetha P. Antioxidant activities of marine algae valoniosis pachynema and sargassum swartzii from the south east coast of india. Int. J. Fish. Aqua. Stud. 2015; 3(2):426-30
23.    Putri, IE, Suyatma NE, Kusumaningrum HD. Film edible antimikrob berbasis isolate protein kedelai dengan ekstrak kunyit dan nanopartikel seng oksida. J. Teknol Dan Industri Pangan. 2018; 29(1): 85-92. Doi:10.6066/jitp.2018.29.1.85
24.    Meyer, B.N., Ferrigni, N.R.,  Putnam, J.E.,  Jacobsen, L.B.,  Nichols, David , McLaughlin, J. Brine Shrimp: A Convenient General Bioassay for Active Plant Constituents. Planta Medica. 1982; 45: 31-4. 10.1055/s-2007-971236.
25.    A.S. Apu, M.A. Muhit, S.M. Tareq, A.H. Pathan, A.T.M. Jamaluddin, M. Ahmed,Antimicrobial Activity and Brine Shrimp Lethality Bioassay of the Leaves Extract of Dillenia indica Linn. Journal of Young Pharmacists. 2010; 2(1): 50-53 https://doi.org/10.4103/0975-1483.62213.
26.    T. Thilagavathi, G. Kathiravan. Phytochemical Analysis and Antimicrobial Activity of Ethonolic Leaf Extract of Ficus racemosa Linn. Research J. Pharm. and Tech. 2017; 10(2): 537-540. doi: 10.5958/0974-360X.2017.00107.X
27.    Fellah, K., Amrouche A., Benmehdi H., Memmou F.. Phenolic profile, antioxidants and kinetic properties of flavonoids and Tannins Fractions isolated from Prunus persica L. leaves growing in Southwest Algeria. Research J. Pharm. and Tech 2019; 12(9): 4365-4372. doi: 10.5958/0974-360X.2019.00751.0
28.    Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). [14] Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology, 152–178. doi:10.1016/s0076-6879(99)99017-1
29.    Yamin, Ruslin, Mistriyani, La Ode Muhammad Fitrawan, Sabarudin, Muhammad Arba. Aktivitas penangkapan radikal bebas ekstrak dan fraksi biji Okra ditentukan dengan metode DPPH (2,2-diphenyl-1-picrylhydrazy). Jurnal Penelitian Farmasi dan Teknologi. 2021; 14(4): 2045-8. doi: 10.52711/0974-360X.2021.00363
30.    Hyunkyung Kim, YunSik Woo, HyungSeok Choi, Mi-Ja Kim, JaeHwan Lee, Dioleylphosphatidylcholine increases the antioxidant properties of ascorbyl palmitate in bulk oils compared to other hydrophilic and lipophilic antioxidants. Food Chemistry. 2021; 349: 129082 https://doi.org/10.1016/j.foodchem.2021.129082
31.    Rekha Rajendran, R Hemachander, T Ezhilarasan, C Keerthana, DL Saroja, KV Saichand, Mohamed Gasim Abdullah. Phytochemical Analysis and In-Vitro Antioxidant Activity of Mimosa pudica Lin., Leaves. Research J. Pharm. and Tech. 2010; 3(2): 551-555.
32.    S. Dhanalakshmi, Abinaya, . Karthiga Devi, Lakshmi. Kajian In Vitro Anti Oksidan Campuran Ekstrak Herbal dengan Metode Nitric Oxide dan DPPH. Research J. Pharm. and Tech. 2017; 10(1): 277-280. doi: 10.5958/0974-360X.2017.00057.9
33.    Sandeep D. S, Prashant Nayak, Jobin Jose, Rishal Relita M, Sumana D. R.. Formulation and Evaluation of Antibacterial Herbal gels of Murraya koenigii Leaves Extract. Research J. Pharm. and Tech. 2017; 10(6): 1798-1801. doi: 10.5958/0974-360X.2017.00317.1
34.    S.B. Prabha, Mohini Rao, M. R. Ramesh Kumar. Evaluation of in vitro Antioxidant, Antibacterial and Anticancer activities of leaf extracts of Cleome rutidosperma. Research J. Pharm. and Tech. 2017; 10(8): 2492-2496. doi: 10.5958/0974-360X.2017.00440.1
35.    Yuan-Feng Zou, Giang Thanh Thi Ho, Karl Egil Malterud, Nhat Hao Tran Le, Kari Tvete Inngjerdingen, Hilde Barsett, Drissa Diallo, Terje Einar Michaelsen, Berit Smestad Paulsen, Enzyme inhibition, antioxidant and immunomodulatory activities, and brine shrimp toxicity of extracts from the root bark, stem bark and leaves of Terminalia macroptera. Journal of Ethnopharmacology. 2014; 155(2): 1219-1226  https://doi.org/10.1016/j.jep.2014.07.004.
36.    Nadar Sowmya, Chouhan Raghavendra Singh, Kosha Patel, Harshil Patel, Tanvi Dodiya, Muthu Kumaradoss Mohan Maruga Raja. Studi in-vitro dan in vivo dari jus Zingiber officinale yang disiapkan secara tradisional untuk aktivitas Antimitotik dan Lethality. Jurnal Penelitian Farmasi dan Teknologi. 2021; 14(9): 4652-6. doi: 10.52711/0974-360X.2021.00808
37.    Sivasankar Palaniappan, Manivasagan Panchanathan, Vijayanand Packiyaraj, Sivakumar Kannan, Sugesh Shanmugam, Poongodi Subramaniam, Maharani Viswanathan, Vijayalakshmi Shanmugam, Thangavel Balasubramanian,Antibacterial and brine shrimp lethality effect of marine actinobacterium Streptomyces sp. CAS72 against human pathogenic bacteria,Asian Pacific Journal of Tropical Disease. 2013; 3(4): 286-293,  https://doi.org/10.1016/S2222-1808(13)60071-7
38.    Samaneh Rahamouz-Haghighi, Khadijeh Bagheri, Ali Sharafi, Mahdi Tavakolizadeh, Neda Mohsen-Pour,Phytochemical screening and Cytotoxicity assessment of Plantago lanceolata L. root extracts on Colorectal cancer cell lines and Brine shrimp larvae and determination of the median lethal dose in mice. South African Journal of Botany. 2022; 149: 740-747  https://doi.org/10.1016/j.sajb.2022.06.058.
39.    Ashpak. M. Tamboli, Kiran. A. Wadkar. Anticancer activity of leaves extracts of Convolvulus pluricaulis and Mimosa rubicaulis (Lam.) by Brine Shrimp Lethality Bioassay. Research J. Pharm. and Tech. 2021; 14(10): 5427-2. doi: 10.52711/0974-360X.2021.00946
40.    M. Obayed Ullah, Mahmuda Haque, Kaniz Fatima Urmi, Abu Hasanat Md. Zulfiker, Elichea Synthi Anita, Momtaj Begum, Kaiser Hamid,Anti–bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh,Asian Pacific Journal of Tropical Biomedicine,. 2013; 3(1): 1-7  https://doi.org/10.1016/S2221-1691(13)60015-5.
41.    Patcharawan Tanamatayarat, Antityrosinase, antioxidative activities, and brine shrimp lethality of ethanolic extracts from Protium serratum (Wall. ex Colebr.) Engl.,Asian Pacific Journal of Tropical Biomedicine. 2016; 6(12): 1050-1055  https://doi.org/10.1016/j.apjtb.2016.10.001.
42.    Mamatha A. Brine Shrimp Lethality Test of Andrographis paniculata. Research J. Pharm. and Tech. 2014; 7(7): 743-745.

Recomonded Articles:

Research Journal of Pharmacy and Technology (RJPT) is an international, peer-reviewed, multidisciplinary journal.... Read more >>>

RNI: CHHENG00387/33/1/2008-TC                     
DOI: 10.5958/0974-360X 

1.3
2021CiteScore
 
56th percentile
Powered by  Scopus


SCImago Journal & Country Rank

Journal Policies & Information


Recent Articles




Tags


Not Available